Assessment of genetic diversity of Burkina Faso sweet grain sorghum using microsatellite markers

Abstract

Sweet grain sorghum [Sorghum bicolor (L.) Moench] is an under-harvested crop produced mainly for its sweet grains in the pasty stage. Little is known of its genetic diversity remains. This study aims to determine the level and structure of the genetic diversity of sweet grain sorghum from Burkina Faso. Thus, 93 accessions were evaluated using 15 polymorphic microsatellite markers. The analysis revealed 49 alleles in total, 6 rare alleles, an average of 3 alleles per locus, a moderate Nei diversity of 0.474, a low level of heterozygosity (0.031) in the collection and very high Wright's fixation index (Fis) of 0.934. The accessions were organized into three genetic groups: A, B and C. Groups A and B were the farthest, with an Fst and a genetic distance of 0.37 and 0.22, respectively, whereas Groups B and C were the closest, with an Fst (genetic differentiation) of 0.279 and a genetic distance of 0.142. This diversity could be exploited in Burkina Faso sweet grain sorghum breeding programs.

Phenotypic variability and correlation estimates for traits of Burkina Faso’ sweet grain sorghum genotypes

“…Most previous studies on sweet grain sorghum focused on its genetic diversity using agromorphological markers (Nebié et al, 2012;Sawadogo et al, 2014aSawadogo et al, , 2014b and microsatellite markers (Sawadogo et al, 2018). Other research efforts have identified mainly sugars responsible for the sweet taste (Sawadogo et al, 2017) and genotypes with high grain yield and of high forage potential (Tiendrebéogo et al, 2018); as well as determine the response of dual-use genotypes to mineral fertilisation (Tiendrebéogo et al, 2020).…”

Genetic Diversity of Jatropha curcas in Burkina Faso Revealed by Microsatellite Markers

“…The DNA extraction was carried out from young leaves of plants installed in the experimental station of the Institute of Rural Development of Gampela based on the methodology used by Sawadogo et al (2018) and Ouedraogo et al (2018). For each accession, the young leaves of a plant were harvested, crushed with a pestle and parafilm paper, and immaculated on an FTA map.…”

Genetic diversity of sorghum (<i>Sorghum bicolor </i>(L) Moench) accessions from thirteen regions of Burkina Faso

“…The diversity among the farmers sorghum landraces was assessed by Ouedraogo (2015) for drought tolerant varieties. More recently, genetic diversity was found by Nerbewende et al (2018) among sweet sorghum accessions collected in Burkina Faso. In fact, the germplasm collected was composed only of farmers' varieties.…”