Assessment of genetic diversity and relationships among Triticum urartu and Triticum boeoticum populations from Iran using IRAP and REMAP markers

Taheri, M.; Alavikia, Siamak; Mohammadi, Seyed Abolghasem; Vahed, Mohammad Moghaddam

doi:10.1007/s10722-018-0660-3

Cited by 14 publications

(11 citation statements)

References 49 publications

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“…Indeed, this result can be explained by the size of the populations and the degree of distributions between different regions [41]. Similar results have been reported previously for genetic diversity in T. urartu germplasm using SSR markers [42], IRAP and REMAP markers [43] and ISSR [25], which can support our findings.…”

Section: Population Genetic Diversitysupporting

confidence: 92%

Assessment of genetic diversity inTriticum urartuThumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers

Gholamian

Etminan

Changizi

et al. 2019

Biotechnology & Biotechnological Equipment

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Section: Population Genetic Diversitysupporting

confidence: 92%

Assessment of genetic diversity inTriticum urartuThumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers

Gholamian

Etminan

Changizi

et al. 2019

Biotechnology & Biotechnological Equipment

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“…Retrotransposon-Microsatellite Amplified Polymorphism system (REMAP). As reported by Taheri et al (2018) in Triticum urartu and Triticum boeoticum, primer namely LTR7286 did not produce DNA band under IRAP system. On the opposite, when the same primer combined with microsatellite primer it produced highest number of polymorphic loci.…”

Section: Optimization Of Irap Markersupporting

confidence: 55%

“…IRAP marker was reported to be less powerful in terms of distinguishing genotypes based on their origin. Taheri et al (2018) reported that IRAP marker was not able to separate Triticum uratu and T. boeoticum genotypes properly based on their geographical distribution. Retrotransposon activities in plant are believed to be triggered by stress-induced by environmental conditions (Lanciano et al 2017, Nie et al 2019.…”

Section: Cluster Analysis and Principal Coordinate Analysis (Pcoa)mentioning

confidence: 99%

Genetic diversity analysis of yardlong bean genotypes (Vigna unguiculata subsp. sesquipedalis) based on IRAP marker

2020

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Abstract. Widyawan MH, Wulandary S, Taryono. 2020. Genetic diversity analysis of yardlong bean genotypes (Vigna unguiculata subsp. sesquipedalis) based on IRAP marker. Biodiversitas 21: 1101-1107. Inter-Retrotransposon Amplified Polymorphism (IRAP) marker is a PCR-based molecular marker that detects polymorphism between retrotransposon sites. This marker has been utilized and successfully assessed genetic diversity in many crop species. Yardlong bean (Vigna unguiculata subsp. sesquipedalis) was an important vegetable legume crop that grown mainly for its fresh pod that rich in nutritional benefits for humans, ultimately dietary fiber and protein. Trends of people awareness to nutritional content of food are increasing, therefore breeding for quality traits is important. Genetic diversity analysis is an important and elementary step in breeding programs in order to determine the breeding strategy. The aims of this research are to perform an optimization of IRAP marker and applied it for genetic diversity analysis in 16 yardlong bean genotypes. Seven primers were used as marker in a pair or single combination and resulted in 11 optimized markers that able to be used for genetic diversity analysis. Sixteen yardlong bean genotypes consisting of commercial cultivars and local genotypes from Indonesia were genotyped using eleven IRAP markers. Marker polymorphism and diversity parameters from each marker i.e. Percentage of Polymorphic Loci (PPL), Expected Heterozygosity (He), Polymorphic Information Content (PIC), Effective Multiplex Ratio (EMR), Marker Index (MI), Discriminating Power (D), and Resolving Power (RP) were calculated. Based on those values, several markers used in this study were considered as informative and efficient in terms of analyzing genetic diversity in yardlong bean. Jaccard's method was used to measure genetic similarity and it is revealed that there is high level of similarity between yardlong bean genotypes used in this study. Thus, it is implied that there is a narrow genetic diversity of yardlong bean genotypes used in this study. Cluster analysis was performed to construct dendrogram based on genetic similarity and classified 16 yardlong genotypes into 4 clusters. Interestingly, majority of the clusters formed were not able to classified genotypes based on their origin. The result of cluster analysis then confirmed by Principal Coordinate Analysis (PCoA) that able to explain 47.76 % of total variation. The results of this study provide a foundation for the genetic diversity analysis based on IRAP marker and genetic improvement in yardlong bean.

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“…The obtained values (PIC = 0.25 and Rp = 28.13) confirmed the usefulness of the combination of REMAP and ISSR primers for the analysis of genetic similarity of genotypes belonging to the genus Aegilops. Taheri et al (2018) analyzed genotypes of the genus Triticum and obtained a PIC of 0.40 using REMAP markers.…”

Section: Discussionmentioning

confidence: 99%

Molecular diversity analysis of genotypes from four Aegilops species based on retrotransposon–microsatellite amplified polymorphism (REMAP) markers

Leśniowska-Nowak

Okoń

Wieremczuk

2020

CEREAL RESEARCH COMMUNICATIONS

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Genetic diversity analysis is an important tool in crop improvement. Species with high genetic diversity are a valuable source of variation used in breeding programs. The aim of this study was to assess the genetic diversity of four species belonging to the genus Aegilops, which are often used to expand the genetic variability of wheat and triticale. Forty-five genotypes belonging to the genus Aegilops were investigated. Within- and among-species genetic diversity was calculated based on REMAP (retrotransposon–microsatellite amplified polymorphism) molecular markers. Obtained results showed that REMAP markers are a powerful method for genetic diversity analysis, which produces a high number of polymorphic bands (96.09% of total bands were polymorphic). Among tested genotypes, Ae. crassa and Ae. vavilovii showed the highest genetic diversity and should be chosen as a valuable source of genetic variation.

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Assessment of genetic diversity and relationships among Triticum urartu and Triticum boeoticum populations from Iran using IRAP and REMAP markers

Cited by 14 publications

References 49 publications

Assessment of genetic diversity inTriticum urartuThumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers

Assessment of genetic diversity inTriticum urartuThumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers

Genetic diversity analysis of yardlong bean genotypes (Vigna unguiculata subsp. sesquipedalis) based on IRAP marker

Molecular diversity analysis of genotypes from four Aegilops species based on retrotransposon–microsatellite amplified polymorphism (REMAP) markers

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