Assessment of Endemic Cota fulvida (Asteraceae) for Phytochemical Composition and Inhibitory Activities against Oxidation, -Amylase, Lipoxygenase, Xanthine Oxidase and Tyrosinase Enzymes

Abstract

In the present work, chemical compositions of essential oil and methanol extract of endemic Cota fulvida (Grierson) Holub were investigated as well as their antioxidant, antidiabetic, antiinflammatory and antimelanogenesis potent. The phytochemical analyses have been performed with GC-MS/FID and LC-MS/MS techniques. The essential oil was characterized with hexadecanoic acid (25.6 %), camphor (6.1 %), caryophyllene oxide (5.3 %), 1,8-cineole (4.9 %) and humulene epoxide (3.9 %). In the extract, phenolic acids, phenylpropanoid dimer and flavonoids were detected. The major constituents of the extracts were found to be 5-feruloylquinic acid, caftaric acid, 3,5-Odicafeoylquinic acid and quercetin rutinoside. The antioxidant activities of the oil and extract were evaluated through scavenging of free radicals, inhibition of linoleic acid peroxidation and superoxide anion radical (O 2-) generated by xanthine -xanthine oxidase (XO) system. The extract showed free radical scavenging activity (IC50 0.131 mg/mL), Trolox equivalent antioxidant capacity (1.33 mM) and inhibited (Inh. 36.3 %) peroxidation of lipids. The oil and extract demonstrated significant hypoglycemic activity via inhibition of porcine pancreatic -amylase. The antiinflammatory effects of the oil and extract via inhibition of 5-LOX enzyme were found as 53.7 % and 23.9 %, respectively. The extract demonstrated moderate inhibitory effect (23.3 %) on oxidation of L-DOPA via inhibition of tyrosinase enzyme.

Lipoxygenase Inhibition by Plant Extracts

“…Content of total phenolic compounds did not differ significantly in the extracts. Similar research was conducted with the oil and extract of Cota fulvida and similar results were obtained [ 32 ]. The essential oil inhibited 5-LOX for 53.7% in comparison to poor inhibition of 23.9% of methanol extract.…”

Phytochemical characterisation of Phlomis linearis Boiss. & Bal and screening for anticholinesterase, antiamylase, antimicrobial, and cytotoxic properties

“…The antimicrobial activity of plant extracts and essential oils (EOs) has accepted for many years. The researchers have documented various publications with the antimicrobial activity of EOs and plant extracts [2,[9][10][11][12], their cytotoxic [7,13], and to management of diabetes as α-amylase inhibitors [14][15][16][17]. in Turkish traditional medicine and consumed as herbal tea in Turkey for carminative and stimulating effects [18].…”

Lipid and essential oil constituents of Cota hamzaoglui (Asteraceae)

“…Distribution of the main compounds groups detected in C. hamzaoglui Özbek & Vural oils is presented on Figure 2. (46), that was characterized with hexadecanoic acid (25.6%), camphor (6.1%), caryophyllene oxide (5.3%), 1,8cineole (4.9%) and humulene epoxide (3.9%). The fatty acids and especially hexadecanoic acid have earlier been observed to be the major constituents in previously studied essential oils obtained from aerial parts of A. dipsacea Bornm. )…”