Assessment of Deoxyribonucleic Acid Fragmentation Index, Testicular Volume, Semen Parameters, and Hormone Profile in Gonadotropin-treated Men With Hypogonadotropic Hypogonadism

Hosseinifar, Hani; Sabbaghian, Marjan; Chehrazi, Mohammad; Modarresi, Tahereh; Alipour, Firouz Jannat; Gilani, Mohammad Ali Sadighi

doi:10.1016/j.urology.2013.06.041

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…It has been shown a relationship between human sperm DNA damage and some semen parameters including concentration, morphology, and motility (Irvine et al, 2000;Lachaud et al, 2004;Henkel et al, 2010;Hosseinifar et al, 2013aHosseinifar et al, , 2014, whereas other studies did not demonstrate such association (Hughes et al, 1996;Henkel et al, 2003;Hosseinifar et al, 2013b). In this study, we also do not detect any significant correlation between DFI and sperm parameters in globozoospermic patients.…”

Section: Discussioncontrasting

confidence: 73%

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

et al. 2015

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SUMMARYThe absence of the acrosome causes the situation which is called globozoospermia. There are a few studies, mostly as case reports, about correlation between levels of sperm DNA damage in patients with total round-headed spermatozoa. We investigated this correlation as well as CMA3 positive spermatozoa in 20 globozoospermic men (with more than 90% round-headed spermatozoa) attending to Royan Institute. Semen samples divided into three parts to semen analysis, to measure DNA fragmentation index (DFI) using sperm chromatin structure assay (SCSA) and to detect CMA3 + sperm cells by chromomycin A3 staining and fluorescent microscopy. Our results showed that there were significant differences in sperm concentration, total sperm motility, and normal morphology between patients and controls group (p < 0.001). Moreover, the average of DFI and CMA3 positive spermatozoa in patients group significantly increases compared with control group (p < 0.001). A significant correlation between DFI and CMA3 + in total population was also detected in patients group (r = 0.45, p = 0.046). To our knowledge, this is the largest study about correlation between DNA damage levels and CMA3 positive spermatozoa with round head sperm cells in total globozoospermic men. It seems that the increase in DNA damage may be because of defective sperm DNA compaction, as we detected CMA3 positive sperm cells in these patients.

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Section: Discussioncontrasting

confidence: 73%

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

et al. 2015

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“…[ 9 ] Among these methods, SCSA is an accurate and fast test that has been shown to be a good predictor of fertility. [ 10 11 12 13 14 ] Statistical thresholds have been established for fertility prognosis when using SCSA procedures in humans. [ 15 ] The different results obtained from SCSA procedures have also been shown to be independent of conventional semen quality measures, and the assay, therefore, makes a contribution to the semen analysis profile.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the impact induced by different incubation time, storage time, storage medium and thawing methods on sperm DNA fragmentation assay: A before–after study

et al. 2022

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“…HMG is a source of FSH to improve spermatogenesis. Numerous patients suffering hypogonadotropic hypogonadism have benefit from the administration of hMG in terms of testicular volume, seminal parameters and pregnancy rate (Finkel, Phillips, & Snyder, ; Hosseseinifar et al, ; Mao et al, ; Yang, Zhang, Dong, Xiong, & Li, ). However, there were relatively few studies concerning the effects of hCG/hMG on the male with normogonadotrophic oligozoospermia.…”

Section: Introductionmentioning

confidence: 99%

Treatment of idiopathic oligozoospermia with combined human chorionic gonadotropin/human menopausal gonadotrophin: A randomised, double‐blinded, placebo‐controlled clinical study

Zhao

et al. 2019

Andrologia

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To evaluate whether hCG/hMG therapy has beneficial effects on idiopathic oligozoospermia in Chinese infertility population. The patients were randomly divided into the treatment group receiving hCG/hMG for 3 months and the placebo group receiving placebo for 3 months. Semen and biochemical analysis was performed, and DNA fragmentation as well as spermatid concentration was evaluated. Administration of hCG/hMG for 3 months could significantly improve sperm concentration, rate of forward motile spermatozoa, total motile sperm count, the percentage of sperm with normal morphology and the rate of spontaneous pregnancy in medium‐ and higher‐level inhibin B group respectively. Moreover, in medium‐ and higher‐level inhibin B group, sperm DNA fragmentation index and spermatid concentration were significantly declined respectively at the end of treatment. However, there were no significant differences in lower‐level inhibin B group before and after treatment in term of seminal parameters, DNA fragmentation and spermatid concentration. HCG/hMG therapy for 3 months has a beneficial effect on a part of male with idiopathic oligozoospermia, and the efficacy of hCG/hMG therapy is associated with the inhibin B level.

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Assessment of Deoxyribonucleic Acid Fragmentation Index, Testicular Volume, Semen Parameters, and Hormone Profile in Gonadotropin-treated Men With Hypogonadotropic Hypogonadism

Cited by 3 publications

References 19 publications

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

Assessment of the impact induced by different incubation time, storage time, storage medium and thawing methods on sperm DNA fragmentation assay: A before–after study

Treatment of idiopathic oligozoospermia with combined human chorionic gonadotropin/human menopausal gonadotrophin: A randomised, double‐blinded, placebo‐controlled clinical study

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