Assessment of Bone Regeneration Using Adipose-Derived Stem Cells in Critical-Size Alveolar Ridge Defects: An Experimental Study in a Dog Model

Alvira-González, Joaquín; Ma, Sánchez-Garcés; Cairó,; Mr, Del Pozo; Cm, Sánchez; Gay‐Escoda, Cosme

doi:10.11607/jomi.4190

Cited by 11 publications

(12 citation statements)

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“…In a previous experimental study using a dog model study, in which the effect of β‐TCP, β‐TCP‐Fn and β‐TCP‐Fn‐ADSCs on alveolar bone dehiscences was compared, only the defects treated with β‐TCP‐Fn‐ADSCs showed a significant increase in the bone regeneration area when the animals were sacrificed at 3 months versus at 1 month ( p = 0.006) (Alvira‐González et al, ).…”

Section: Discussionmentioning

confidence: 98%

“…Twenty‐four hours before to surgery, 500 µl of Fn solution (10 µg in Dulbecco's modified Eagle medium [DMEM] 1 g/L) was added per gram of bone graft and incubated for 24 hr at 37ºC. The coating solution was then eliminated, and the grafts were washed with Dulbecco's phosphate‐buffered saline (DPBS) and stored in multi‐dose blisters, in sterile conditions, ready to be refrigerated, transported and used as previously reported (Alvira‐González et al, ; Sánchez‐Garcés et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…In previous studies of our group, the bone regeneration potential of β‐TCP‐Fn with autologous adipose‐derived stem cells (β‐TCP‐Fn‐ADSCs) was examined in critical‐sized defects (CSDs) of alveolar ridges in a dog model (Alvira‐González et al, ) and in dehiscence‐type defects associated with dental implants (Sánchez‐Garcés et al, ). The use of ADSCs does not seem to improve the area of bone regeneration and bone‐implant contact (BIC) and did not entail an advantage as compared with other biomaterials.…”

Section: Introductionmentioning

confidence: 99%

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Guided bone regeneration using beta‐tricalcium phosphate with and without fibronectin—An experimental study in rats

Escoda-Francolí

Sánchez-Garcés

Gimeno-Sandig

et al. 2018

Clinical Oral Implants Res

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Objective: This histomorphometric study compared bone regeneration potential of beta-tricalcium phosphate with fibronectin (β-TCP-Fn) in critical-sized calvarial defects (CSDs) in rats to assess whether fibronectin (Fn) improved new bone formation. Material and methods: Critical-sized calvarial defects were created in 30 adult male Sprague Dawley rats, which were divided into four groups according to the time of euthanasia (6 or 8 weeks of healing) and type of filling (β-TCP-Fn/6 weeks, β-TCP/6 weeks, β-TCP-Fn/8 weeks and β-TCP/8 weeks). The primary variables related to new bone formation were augmented area (AA) and gained tissue (GT; sum of mineralized bone matrix [MBM] and bone substitute [BS]). Secondary variables were the diameter of the defect, MBM, non-mineralized tissue (NMT) and BS. Results: A total of 29 rats and 58 histological samples were evaluated, 28 (48.3%) samples obtained at 6 weeks and 30 (51.7%) at 8 weeks, homogeneously distributed between right and left sides. Thirteen (22.4%) were treated with β-TCP-Fn, 16 (27.6%)with β-TCP and 29 (50%) were controls. At 8 weeks, histomorphometric analysis showed significant differences in AA using β-TCP and β-TCP-Fn versus controls (p = 0.001 and p = 0.005, respectively). Bone turnover expressed as % within the target area was slightly higher but not statistically significant in the β-TCP-Fn than in β-TCP (MBM) at 6 weeks versus 8 weeks (p = 0.067 and p = 0.335, respectively).Finally, the total GT area in mm 2 was higher using β-TCP-Fn as compared to β-TCP (p = 0.044). | 1039ESCODA-FRANCOLÍ Et AL.

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Guided bone regeneration using beta‐tricalcium phosphate with and without fibronectin—An experimental study in rats

Escoda-Francolí

Sánchez-Garcés

Gimeno-Sandig

et al. 2018

Clinical Oral Implants Res

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“…Human adipose-derived mesenchymal stem cells (hADSCs) are a type of adult mesenchymal stem cells (MSCs) that have the plasticity to differentiate into osteoblasts, chondrocytes and adipocytes in response to the appropriate conditions ( 1 - 3 ). In recent studies, hADSCs have been successfully induced into an osteogenic lineage in vitro and have been used as seed cells to effectively repair bone defects ( 4 , 5 ). However, the molecular mechanisms governing the osteogenic differentiation of hADSCs are not fully elucidated, and thus investigating the potential mechanisms is of great importance.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway

Zhang

Zhou

et al. 2020

Int J Mol Med

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MicroRNAs (miRNAs) are known to have regulatory roles in the osteogenic differentiation of various mesenchymal stem cells (MScs), although their regulatory role on human adipose-derived mesenchymal stem cells (hAdScs) remains unclear. The aim of the present study was to investigate the biological function and underlying molecular mechanism of miRNAs in regulating the osteogenic differentiation of hAdScs using microarray assay. hAdScs differentiated into osteoblasts under culture with osteogenic medium, with an increase observed in calcium deposits and alkaline phosphatase activity. The mRNA levels of bone sialoprotein, osteopontin and osteocalcin increased, whereas Runt-related transcription factor-2 expression decreased during osteogenic differentiation. In addition, miR-143 was markedly downregulated during osteogenic differentiation, while miR-143 overexpression inhibited and miR-143 knockdown enhanced this process. miR-143 overexpression also blocked extracellular signal-regulated kinase 1/2 (ERK1/2) pathway activation, while miR-143 inhibition enhanced it. The promoting effects of miR-143 knockdown on the osteogenic differentiation of hAdScs were partly diminished by the mitogen-activated protein kinase (MEK) inhibitors U0126 and Pd98059. Bioinformatics analysis further revealed that miR-143 targets k-Ras and directly binds to the 3'-untranslated region of its mRNA. Inhibition of miR-143 enhanced the activation of the k-Ras/MEK/ERK pathway during osteogenic differentiation, whereas miR-143 overexpression had the opposite effect. collectively, these results demonstrated that miR-143 negatively regulates the osteogenic differentiation of hAdScs through the k-Ras/MEK/ERK pathway, providing further insight into the underlying molecular mechanisms.

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“…In the scientific literature, ASCs have successfully been combined with biomaterials, such as β-tricalcium phosphate (β-TCP) [ 11 ], bioactive glass (BAG) [ 12 ], and platelet-rich plasma (PRP) [ 13 ], to improve bone regeneration in animal models: Such studies on animals, together with several case reports on humans, provided interesting results in the field of bone regeneration [ 14 , 15 , 16 , 17 , 18 , 19 ]. These results describe a new approach to bone tissue regeneration, based on ASCs/scaffold constructs; however, a deep analysis of the studies reported in the literature would be useful to understand the real limits and perspectives of ASCs in bone replacement, from a clinical and molecular point of view.…”

Section: Introductionmentioning

confidence: 99%

Adipose Tissue as a Strategic Source of Mesenchymal Stem Cells in Bone Regeneration: A Topical Review on the Most Promising Craniomaxillofacial Applications

Paduano¹,

Marrelli

Amantea

et al. 2017

IJMS

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Bone regeneration in craniomaxillofacial surgery represents an issue that involves both surgical and aesthetic aspects. The most recent studies on bone tissue engineering involving adipose-derived stromal/stem cells (ASCs) have clearly demonstrated that such cells can play a crucial role in the treatment of craniomaxillofacial defects, given their strong commitment towards the osteogenic phenotype. A deeper knowledge of the molecular mechanisms underlying ASCs is crucial for a correct understanding of the potentialities of ASCs-based therapies in the most complex maxillofacial applications. In this topical review, we analyzed the molecular mechanisms of ASCs related to their support toward angiogenesis and osteogenesis, during bone regeneration. Moreover, we analyzed both case reports and clinical trials reporting the most promising clinical applications of ASCs in the treatment of craniomaxillofacial defects. Our study aimed to report the main molecular and clinical features shown by ASCs, used as a therapeutic support in bone engineering, as compared to the use of conventional autologous and allogeneic bone grafts.

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Assessment of Bone Regeneration Using Adipose-Derived Stem Cells in Critical-Size Alveolar Ridge Defects: An Experimental Study in a Dog Model

Cited by 11 publications

References 0 publications

Guided bone regeneration using beta‐tricalcium phosphate with and without fibronectin—An experimental study in rats

Guided bone regeneration using beta‐tricalcium phosphate with and without fibronectin—An experimental study in rats

Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway

Adipose Tissue as a Strategic Source of Mesenchymal Stem Cells in Bone Regeneration: A Topical Review on the Most Promising Craniomaxillofacial Applications

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