Assessment of Bisphenol A (BPA) neurotoxicity in vitro with mouse embryonic stem cells

Abstract

The adverse effects of environmental pollution on our well-being have been intensively studied with many in vitro and in vivo systems. In our group, we focus on stem cell toxicology due to the multitude of embryonic stem cell (ESC) properties which can be exerted in toxicity assays. In fact, ESCs can differentiate in culture to mimic embryonic development in vivo, or specifically to virtually any kind of somatic cells. Here, we used the toxicant Bisphenol A (BPA), a chemical known as a hazard to infants and children, and showed that our stem cell toxicology system was able to efficiently recapitulate most of the toxic effects of BPA previously detected by in vitro system or animal tests. More precisely, we demonstrated that BPA affected the proper specification of germ layers during our in vitro mimicking of the embryonic development, as well as the establishment of neural ectoderm and neural progenitor cells.

Oviduct: roles in fertilization and early embryo development

“…However, BPA is still being used as part of the plastic containers for embryo cultures (Hunt et al 2003, Berger et al 2010, Varayoud et al 2011). Studies indicated a broad range of effects on the embryos upon BPA exposure, such as altered developmental rate and cell death (Ferris et al 2016) and toxicity to the neural progenitor cells (Yin et al 2015). BPA-free replacement products from the industry still diffuse out estrogenic chemicals, such as BPAF (the fluorinated form of BPA) (Bittner et al 2014), which may pose a significant hazard to the embryos.…”

Estrogenic Endocrine Disrupting Chemicals Influencing NRF1 Regulated Gene Networks in the Development of Complex Human Brain Diseases

“…In utero exposure to BPA in mice was shown to reduce dendritic spine densities in the hippocampal CA1 region, regardless of its dose [82]. BPA was demonstrated to impair neural ectoderm specification and neural progenitor cells in mouse embryonic stem cells [83]. In a study using Caenorhabditis elegans to examine early embryogenesis exposure to BPA and BPS into adulthood, it was shown that changes in behavior and learning were followed into adulthood [84].…”

Sensitive determination of typical phenols in environmental water samples by magnetic solid‐phase extraction with polyaniline@SiO2@Fe as the adsorbents before HPLC

“…BPA would enter into the environment by the industrial emissions, the disposal of PVC materials, and the using process of the products containing BPA . BPA has toxic effect on the reproductive system and nervous system of biological organisms . Besides, BPA has certain affinity with estrogen receptor , which can lead to the occurrence of ovarian cancer, breast cancer, and other cancers.…”