Assessing the polymorphism of DHFR gene from Plasmodium falciparum in the south of Cte dIvoire

OlÃ©fongo, Dagnogo; Berenger, Ako Aristide; Brice, Bla Kouakou; Noel, Dago Dougba; David, Coulibaly N’Golo; Coulibaly, Boubacar; AndrÃ©, TourÃ© Offianan; Joseph, Djaman Allico

doi:10.5897/ajmr2020.9309

Cited by 1 publication

(1 citation statement)

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“…Similar results were reported by Djaman et al [26]. Recent work by Dagnogo et al in Côte d'Ivoire reported a predominance of MNK genotype (wild type) with a frequency of 62% [27]. In contrast, single mutant, double mutant and triple mutant genotypes were observed with prevalence of 12%, 6% and 18% respectively.…”

Section: Discussionsupporting

confidence: 87%

Genetic Polymorphisms in Plasmodium falciparum Chloroquine Resistance Gene, pfcrt in Massakory (Chad)

Taïsso¹,

Berenger²,

Souleymane³

et al. 2022

AJBIO

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Background and Objective: In 2005, Chad, like several other WHO countries, withdrew chloroquine as a first-line treatment for Plasmodium falciparum malaria in response to WHO recommendations related to the reason for the increase in treatment failures and the global spread of chloroquine resistance. Artemisinin-based combination therapy (ACTs), Artemether-lumefantrine, has replaced chloroquine as the first-choice treatment for malaria. The present study assessed pfcrt polymorphism in Plasmodium falciparum isolates in Massakory. Methodology and Results: Blood samples for PCR analysis were collected on Whatman 3MM filter paper in Massakory during a therapeutic efficacy study (TES) conducted from December 14, 2019 to March 14, 2020. Genomic DNA was extracted from 113 dried blood spots with the QIAamp DNA Micro Kit (Qiagen, Valencia, CA) as per manufacturer's protocol and amplified by nested-PCR with pfcrt specific primer. The amplification products were revealed by electrophoresis on 2% agarose gel and then sequenced according to Sanger method. A total of 71 sequences were readable. The pfcrt analysis showed that of the 71 readable sequences, high mutation prevalence: 66 (92.96%) IET, 2 (4.22%) IDT and 3 (4.22%) MNK wild pfcrt isolates. Conclusion: These results challenge the highest health authorities in the country. The government, through the Ministry of Public Health and National Solidarity and the National Malaria Control Program, must raise awareness for the effective withdrawal of chloroquine. This action will promote on the one hand the re-emergence of parasites sensitive to chloroquine, and on the other hand make possible the reintroduction of chloroquine in the treatment of simple malaria after the suppression of drug pressure.

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Section: Discussionsupporting

confidence: 87%

Genetic Polymorphisms in Plasmodium falciparum Chloroquine Resistance Gene, pfcrt in Massakory (Chad)

Taïsso¹,

Berenger²,

Souleymane³

et al. 2022

AJBIO

View full text Add to dashboard Cite

show abstract

Assessing the polymorphism of DHFR gene from Plasmodium falciparum in the south of Cte dIvoire

Cited by 1 publication

References 10 publications

Genetic Polymorphisms in<i> Plasmodium falciparum </i>Chloroquine Resistance Gene<i>, pfcrt </i>in Massakory (Chad)

Genetic Polymorphisms in<i> Plasmodium falciparum </i>Chloroquine Resistance Gene<i>, pfcrt </i>in Massakory (Chad)

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Assessing the polymorphism of DHFR gene from Plasmodium falciparum in the south of Cte dIvoire

Cited by 1 publication

References 10 publications

Genetic Polymorphisms in&lt;i&gt; Plasmodium falciparum &lt;/i&gt;Chloroquine Resistance Gene&lt;i&gt;, pfcrt &lt;/i&gt;in Massakory (Chad)

Genetic Polymorphisms in&lt;i&gt; Plasmodium falciparum &lt;/i&gt;Chloroquine Resistance Gene&lt;i&gt;, pfcrt &lt;/i&gt;in Massakory (Chad)

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Genetic Polymorphisms in<i> Plasmodium falciparum </i>Chloroquine Resistance Gene<i>, pfcrt </i>in Massakory (Chad)

Genetic Polymorphisms in<i> Plasmodium falciparum </i>Chloroquine Resistance Gene<i>, pfcrt </i>in Massakory (Chad)