Assessing the genetic diversity of Spanish Allium cepa landraces for onion breeding using microsatellite markers

Giménez, Cristina Mallor; Arnedo-Andrés, M. S.; Garcés-Claver, Ana

doi:10.1016/j.scienta.2014.02.040

Cited by 42 publications

(54 citation statements)

References 31 publications

(46 reference statements)

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“…The bands of ACM315 (290 bp), ACM238 (153 bp) and ACM300 (184 bp, 207 bp, 216 bp) were excluded from further evaluation because of being monomorphic across the studied set of 96 accessions. Similarly, ACM300 produced monomorphic bands in Spanish onions (Mallor et al 2014). The observed allele sizes in this study for the EST-SSR markers were similar to expected values (McCallum et al 2008) but not exactly the same.…”

Section: Resultssupporting

confidence: 86%

“…Other authors reported different allele sizes compared to those described in the original publications. For example, ACM004 gave bands between 201 bp and 212 bp in Czech onion varieties (Mitrová et al 2015); 220 bp and 230 bp in Spanish onion accessions (Mallor et al 2014); and 201 bp and 210 bp in Indian onion accessions (Khar et al 2011). We observed 224 bp and 256 bp bands in Turkish onion accessions using the same marker.…”

Section: Resultsmentioning

confidence: 49%

“…The ACM238 produced the highest number of bands (14). The EST-SSRs markers showed a higher percentage of amplification than in previous reports (Jakše et al 2005;Khar et al 2011;Mallor et al 2014;Mitrová et al 2015). The bands of ACM315 (290 bp), ACM238 (153 bp) and ACM300 (184 bp, 207 bp, 216 bp) were excluded from further evaluation because of being monomorphic across the studied set of 96 accessions.…”

Section: Resultsmentioning

confidence: 72%

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Molecular characterization of Turkish onion germplasm using SSR markers

Hancı¹,

Gökçe²

2016

Czech J. Genet. Plant Breed.

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Hanci F., Gökçe A.F. (2016): Molecular characterization of Turkish onion germplasm using SSR markers. Czech J. Genet. Plant Breed., 52: 71-76.In this study, molecular markers were used for the first time to study the genetic diversity of Turkish onion (Allium cepa L.) accessions. We used 83 turkish landraces, 3 breeding lines and 10 commercial varieties, in total 96 accessions, to study the genetic diversity. Bulked samples of 10 seeds were used to generate a representative profile of the accessions. Variability was evaluated at 46 microsatellite loci. We identified 308 alleles with these markers, out of which 303 were polymorphic. A dendrogram based on the UPGMA analysis grouped the 96 accessions into five main clusters. Dice's similarity coefficient ranged from 0.407 to 0.767 with an average of 0.587. The results showed that 44 out of the 46 SSR markers were convenient and polymorphic enough to distinguish all the studied accessions.

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 49%

Section: Resultsmentioning

confidence: 72%

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Molecular characterization of Turkish onion germplasm using SSR markers

Hancı¹,

Gökçe²

2016

Czech J. Genet. Plant Breed.

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“…SSR (single sequence repeats) markers are the most popular, owing to their reliability, robustness, and cost effectiveness. They have been used for diversity estimates (Mallor et al 2014), cultivar identification (Anandhan et al 2014, and diversity studies (McCallum et al 2008).…”

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confidence: 99%

The selection and validation of a marker set for the differentiation of onion cultivars from the Czech Republic

Mitrová¹,

Svoboda²,

Ovesná³

2015

Czech J. Genet. Plant Breed.

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Mitrová K., Svoboda P., Ovesná J. (2015): The selection and validation of a marker set for the differentiation of onion cultivars from the Czech Republic. Czech J. Genet. Plant Breed., 51: 62-67.The onion (Allium cepa L.) constitutes an important part of the human diet and is of economic importance on the vegetable market. Methods that allow unambiguous differentiation of cultivars and breeding lines are required for market control and the protection of plant breeders' rights. DNA analyses, such as single sequence repeat polymorphism (SSRs), represent such a tool. Based on the evaluation of polymorphism in 21 SSR loci, we established a panel of 15 easy to score SSR markers that differentiated 16 commercial onion cultivars in the Czech Republic. The polymorphism ranged from 2-3 alleles per locus, indicating a low level of diversity among the cultivars. The panel can also be used to evaluate genetic resources.

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“…Keragaman yang dihasilkan pada penelitian ini sedikit lebih tinggi dibandingkan hasil penelitian Mallor et al (2014) Kuning, dan Sembrani). Kelompok II terdiri atas 4 genotipe yaitu varietas Batu Ijo, Ilokos, Manjung (varietas yang sudah dilepas oleh Kementan RI) dan koleksi bawang merah dari Cirebon (Jawa Barat).…”

Section: Keragaman Bawang Merah Berdasarkan Marka Issrunclassified