Assessing Nitric Oxide (NO) in Higher Plants: An Outline

Corpas, Francisco J.; Palma, José M.

doi:10.3390/nitrogen1010003

Cited by 46 publications

(22 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This might be because of the possible role of AtWAKL10 in RNS scavenging which may be due to its many fold induction (>124) in response to CysNO. Therefore a perturbation in the function may result more nitrosative stress which ultimately results in compromised plant growth as suggested by many reports (Corpas & Palma, 2018; Lee et al, 2008; Lindermayr, 2017). The difference in response of atwakl10 plants to different NO donors might be because of the differential accumulation of intracellular NO due to different NO release rates of NO donors (He & Frost, 2016).…”

Section: Discussionmentioning

confidence: 98%

Differential expression of AtWAKL10 in response to nitric oxide suggests a putative role in biotic and abiotic stress responses

Bot

Mun

Imran

et al. 2019

PeerJ

View full text Add to dashboard Cite

Plant defense against pathogens and abiotic stresses is regulated differentially by communicating signal transduction pathways in which nitric oxide (NO) plays a key role. Here, we show the biological role of Arabidopsis thaliana wall-associated kinase (AtWAK) Like10 (AtWAKL10) that exhibits greater than a 100-fold change in transcript accumulation in response to the NO donor S-nitroso-L-cysteine (CysNO), identified from high throughput RNA-seq based transcriptome analysis. Loss of AtWAKL10 function showed a similar phenotype to wild type (WT) with, however, less branching. The growth of atwakl10 on media supplemented with oxidative or nitrosative stress resulted in differential results with improved growth following treatment with CysNO but reduced growth in response to S-nitrosoglutatione (GSNO) and methyl-viologen. Further, atwakl10 plants exhibited increased susceptibility to virulent Pseudomonas syringae pv tomato (Pst) DC3000 with a significant increase in pathogen growth and decrease in PR1 transcript accumulation compared to WT overtime. Similar results were found in response to Pst DC3000 avrB, resulting in increased cell death as shown by increased electrolyte leakage in atwakl10. Furthermore, atwakl10 also showed increased reactive oxygen species accumulation following Pst DC3000 avrB inoculation. Promoter analysis of AtWAKL10 showed transcription factor (TF) binding sites for biotic and abiotic stress-related TFs. Further investigation into the role of AtWAKL10 in abiotic stresses showed that following two weeks water-withholding drought condition most of the atwakl10 plants got wilted; however, the majority (60%) of these plants recovered following re-watering. In contrast, in response to salinity stress, atwakl10 showed reduced germination under 150 mM salt stress compared to WT, suggesting that NO-induced AtWAKL10 differentially regulates different abiotic stresses. Taken together, this study further elucidates the importance of NO-induced changes in gene expression and their role in plant biotic and abiotic stress tolerance.

show abstract

Section: Discussionmentioning

confidence: 98%

Differential expression of AtWAKL10 in response to nitric oxide suggests a putative role in biotic and abiotic stress responses

Bot

Mun

Imran

et al. 2019

PeerJ

View full text Add to dashboard Cite

show abstract

“…In particular, nitric oxide (NO), a versatile signaling molecule has been found to play critical roles in plant defense reactions including induction of antioxidant potential under various plant abiotic stresses [ 26 , 27 ]. Adequate evidence substantiates a wide range of roles of NO in plant physiological processes including germination, photosynthesis, and defense mechanism both under normal and environmental stresses [ 28 , 29 ]. In plants, the cell-protective role of NO has been tested with DNA, proteins, lipids, and chlorophyll [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Nitric Oxide Pre-Treatment Advances Seed Germination and Alleviates Copper-Induced Photosynthetic Inhibition in Indian Mustard

Rather

Mir

Masood

et al. 2020

Plants

View full text Add to dashboard Cite

This investigation tested the efficiency of nitric oxide (NO) in alleviation of Cu-induced adverse impacts on seed germination and photosynthesis in Indian mustard (Brassica juncea L.). Pre-treatment of B. juncea seeds with sodium nitroprusside (SNP; NO donor) significantly improved the seed germination rate and also alleviated Cu-accrued oxidative stress. However, in the absence of NO, Cu caused a higher reduction in seed germination rate. The presence of NO strengthened the antioxidant defense system (glutathione reductase, ascorbate peroxidase, and superoxide dismutase) and thereby sustained the lower lipid peroxidation, reduced H2O2 content, and thiobarbituric acid reactive substances in Cu-exposed seeds. NO pre-treated seeds also retained a higher amylase activity and exhibited an improved seed germination rate. This effect of NO under Cu stress was also seen in plants originated from the NO pre-treated seeds, where the role of NO pre-treatment was reflected in the improved photosynthetic potential of B. juncea. Overall, NO pre-treatment not only improved the germination rate in seeds but also carried its effects in the grown seedlings evidenced as improved photosynthesis and growth. Potential mechanisms involved in the action of NO pre-treatment included NO-mediated significant strengthening of the antioxidant defense system and decreases in Cu-caused oxidative stress parameters.

show abstract

“…As an example, it maintains reactive oxygen species (ROS) at low levels through changing the activities of key antioxidant enzymes (Niu and Guo, 2012;Groß et al, 2013;Ahmad et al, 2016). Various investigations have shown that NO is implicated in several hormonal and biological responses in plants (Hasanuzzaman et al, 2012;Corpas and Palma, 2018), but the likely contribution of NO to thiamine-induced antioxidant defense needs to be elucidated. Thus, this study aimed to assess if thiamineinduced synthesis of NO was implicated in alleviation of B stress in pepper plants.…”

Section: Introductionmentioning

confidence: 99%

Thiamine-induced nitric oxide improves tolerance to boron toxicity in pepper plants by enhancing antioxidants

Kaya¹,

Aslan²,

Uğurlar³

et al. 2020

Turk J Agric For

View full text Add to dashboard Cite

The role of thiamine (THI)-induced nitric oxide (NO) synthesis in the improvement of tolerance to boron (B) toxicity in pepper (Capsicum annuum L.) was studied. A solution of THI (50 or 100 mg L -1 ) was sprayed to the foliage of pepper plants exposed to high B regime (2.0 mM H 3 BO 3 ) once a week for 4 weeks. A scavenger of NO (0.1 mM), cPTIO, along with THI was sprayed once a week to ascertain whether or not endogenous NO played a role in the alleviation of B stress in pepper plants by THI. There were significant reductions in plant dry weight, PSII quantum efficiency (F v /F m ), total chlorophyll, leaf calcium (Ca 2+ ) and potassium (K + ) contents as well as leaf water potential by 47.57%, 28.78%, 34.42%, 45.45%, 55.53%, and 471.4%, respectively, but there was a significant increase in the leaf proline, ascorbate (AsA), glutathione (GSH), hydrogen peroxide, malondialdehyde, electron leakage, B levels and NO by 2.6-, 2.3-, 1.9, 3.7-, 5.6-, 2.2-, 3.6-, and 3.0-fold, respectively in B-stressed plants. Both treatments of THI mitigated B-induced oxidative damage and led to a further increase in NO synthesis. The positive effect of THI on plants grown under B stress was fully eliminated by the cPTIO application by lowering leaf NO content. These findings exhibit that NO may function as a downstream signal in THI-induced tolerance to B toxicity in pepper plants by reversing oxidative stress, enhancing the antioxidant defense mechanism and sustaining mineral nutrient homeostasis. Thus, NO and THI both contributed to improved B toxicity tolerance in pepper plants.

show abstract

Assessing Nitric Oxide (NO) in Higher Plants: An Outline

Cited by 46 publications

References 74 publications

Differential expression of AtWAKL10 in response to nitric oxide suggests a putative role in biotic and abiotic stress responses

Differential expression of AtWAKL10 in response to nitric oxide suggests a putative role in biotic and abiotic stress responses

Nitric Oxide Pre-Treatment Advances Seed Germination and Alleviates Copper-Induced Photosynthetic Inhibition in Indian Mustard

Thiamine-induced nitric oxide improves tolerance to boron toxicity in pepper plants by enhancing antioxidants

Contact Info

Product

Resources

About