Assessing genetic diversity in 23 early Polish oat cultivars based on molecular and morphological studies

Boczkowska, Maja; Nowosielski, Jarosław; Nowosielska, D.; Podyma, Wiesław

doi:10.1007/s10722-014-0087-4

Cited by 25 publications

(26 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The difference could result from lower number of cultivars from an earlier period which have survived till today. Analogous results were obtained during the evaluation of morphological differentiation of these materials (Boczkowska et al 2014). The values of diversity coefficients clearly indicated that the old cultivars were undoubtedly formed by breeding programs, but criteria were not as stringent as those used in modern breeding.…”

Section: Discussionsupporting

confidence: 55%

“…1) and, what is more important, they represented completely different phenotypes. Plants belonging to Koscielecki cultivar had white lemma colour, equilateral type of panicle while Grzywacz Póxny Wolymski had yellow colour of lemma and unilateral panicle (Boczkowska et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…before the World War II. This was a direct continuation of the assessment of genetic and morphological diversity of common oat cultivars bred in Poland in the twentieth century (Nowosielska 2006;Boczkowska et al 2014). This is also a part of major project that will describe the genetic variation of the whole Avena collection in Polish national repository.…”

Section: Introductionmentioning

confidence: 94%

See 2 more Smart Citations

Studies on genetic variation within old Polish cultivars of common oat

Boczkowska¹,

Harasimiuk²,

Onysk³

2015

Cereal Research Communications

Self Cite

View full text Add to dashboard Cite

The gene pool of Polish oat cultivars is quite narrow. Obsolete cultivars bred at the beginning of the 20 th century could be one of the "new" sources of genetic diversity. Re-using them in breeding programs might be much easier than interspecific hybridization with wild relatives. The aim of this study was to assess genetic variation within cultivars bred in Poland before 1939. Molecular analysis was carried out using ISSR markers. The obtained results have shown the old cultivars are much more heterogenic than the advanced ones. Distinctiveness of the gene pool of these accessions in relation to the modern Polish cultivar and landrace was also observed. UPGMA analysis has shown, despite the considerable time lapse, the tested cultivars retain their genetic integrity. Based on archive pedigree data it was found that most of the analysed accessions had diverse origin. Low value of total genetic variation of the old cultivars collection may indicate the gene pool of the primeval oat forms which were brought and grown in Poland was quite narrow. Both breeding history and the results presented in this paper suggest the restoration of old cultivars to Polish breeding should be one of the main tasks of gene bank in the future. According to the monitoring of accession sharing conducted by the national gene bank, breeders' interest in old cultivars and landraces was and still is neglected.

show abstract

Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

See 1 more Smart Citation

Studies on genetic variation within old Polish cultivars of common oat

Boczkowska¹,

Harasimiuk²,

Onysk³

2015

Cereal Research Communications

Self Cite

View full text Add to dashboard Cite

show abstract

“…Analysis of genetic diversity can assist in reliable classification of collections required for crop improvement (Mohammadi and Prasanna 2003;Manohar and Murthy 2012). Morphological analysis has been used for characterizing large data sets and such description has become a valuable source of information for agronomic and breeding programs (Boczkowska et al 2014). In the present study, the morphological classification of 36 accessions revealed a great diversity in fruit morphological traits across India.…”

Section: Discussionmentioning

confidence: 68%

A comparative assessment of morphological and molecular diversity among Sechium edule (Jacq.) Sw. accessions in India

et al. 2017

View full text Add to dashboard Cite

Genetic variation of 36 Sechium edule accessions collected across 12 states in India was assessed using morphological traits and DAMD markers. Eighteen fruit morphological traits (both qualitative and quantitative) were evaluated to confirm the variations in the present collection. Quantitative traits showed major variations with respect to fruit weight (7.85-498.33 g/fruit), fruit length (5.8-15 cm/fruit), fruit diameter (6-28 cm/fruit) and length of the spine (0-5 cm). Qualitative traits were also diverse in fruit colour, shape, spine density, reticulation, flexibility of spine and furrow depth. The first six principle components showed 82.88% variation in the principal component analysis. The principal component analysis revealed that fruit weight, fruit width, fruit diameter, fruit shape, length of spine, spine density and furrow depth had a significant contribution to the total variation. The DNA analysis performed using DAMD primers were used for deducing the diversity at DNA level. The collection produced 102 bands out of which 97 were polymorphic and the percentage polymorphism ranged between 66.66 and 100 per primer. Discrete pattern of clustering was obtained using UPGMA method of complete linkage percent disagreement revealing high diversity among the collected accessions. Thus, the present study indicates that molecular and morphological marker map would improve our knowledge of S.edule and would facilitate efforts to breed improved S. edule cultivars.

show abstract

“…In general, the DC lines have lower variation than the STH pool. Interestingly, that similar analyses performed on 23 old cultivars using AFLP, ISSR and RAPD markers failed in any significant grouping of the analysed materials (Boczkowska et al, 2014). The data demonstrates that the analysed materials show enough variation useful for breeding purposes.…”

Section: Discussionmentioning

confidence: 51%

Genetic Similarity Assessment among Selected Naked Oat Cultivars and Breeding Lines Using ISSR Markers

Paczos-Grzęda

Bednarek

Koroluk

et al. 2014

Not Bot Hort Agrobot Cluj

View full text Add to dashboard Cite

Naked oat refers to a variety of Avena sativa with lemma and palea separating from the grains. Its spikelets are multiflorous and morphologically different from the husked oat. Problems with preharvest sprouting, threshability, rancidity, a wide range of kernel sizes, as well as its relatively low tolerance to limited soil water content, are its main drawbacks. Nevertheless it could be an alternative to a conventional oat. Unfortunately, its genetic variation is still poorly recognized. In the given study a set of 26 naked oat cultivars and lines were analyzed with 25 inter-simple sequence repeats (ISSR) primers that amplified as many as 429 DNA fragments among which 204 were polymorphic. The average number of markers amplified per primer pair and polymorphism information content (PIC) value equaled to eight and 0.23, respectively. Forty four unique PCR products were identified for different genotypes. While Unweighted Pair-Group Method with Arithmetic Mean failed to distinguish the materials into main clusters it demonstrated that cultivars 'Akt', 'Polar', 'Cacko', 'Siwek', 'Nagus' and most of the DC lines were within a single group. Moreover, the cultivars that were closely related based on their breeding pedigree (related to 'Akt') were close to each other. Principal Coordinate Analysis explained 54.1% of variance and was in good agreement with the UPGMA. ISSR markers could be used for the evaluation of genetic similarity of cultivars and lines as well as the differentiation of individual genotypes. This study demonstrated that the available A. sativa naked type genetic pool is relatively wide and have the potential for further breeding progress.

show abstract

Assessing genetic diversity in 23 early Polish oat cultivars based on molecular and morphological studies

Cited by 25 publications

References 43 publications

Studies on genetic variation within old Polish cultivars of common oat

Studies on genetic variation within old Polish cultivars of common oat

A comparative assessment of morphological and molecular diversity among Sechium edule (Jacq.) Sw. accessions in India

Genetic Similarity Assessment among Selected Naked Oat Cultivars and Breeding Lines Using ISSR Markers

Contact Info

Product

Resources

About