2016
DOI: 10.1007/s10616-016-0005-z View full text |Buy / Rent full text
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Abstract: Hyperuricemia is characterized by the high uric acid (UA) level in serum (or plasma) and has been considered to be an important risk factor for gout. In the present study, we have attempted to construct an assay system for UA production in vitro employing cultured AML12 hepatocytes. UA levels in balanced salt solution (BSS) in the presence of UA precursor nucleosides, adenosine, inosine, guanosine and xanthine, at 12.5, 25, and 100 µM were significantly higher than BSS alone and their effects were dose-depende… Show more

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“…They were also housed in the same conditions of 23 ± 1°C, relative humidity of 55% and 12-h light/12-h dark cycle (lights on 7:00-19:00 h) and given standard mouse pellets and tap water ad libitum. Following a previous study (Adachi, Yoshizawa, & Yagasaki, 2017;Niu et al, 2017), uricase inhibitor and large amounts of purine were administered to develop a mouse model of hyperuricemia. All mice were divided into five groups of 12 mice each and gavaged with the same volume agents for 8 days.…”
Section: Experiments Performed In Oteracil Potassium (Oxo)-induced Hymentioning
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“…They were also housed in the same conditions of 23 ± 1°C, relative humidity of 55% and 12-h light/12-h dark cycle (lights on 7:00-19:00 h) and given standard mouse pellets and tap water ad libitum. Following a previous study (Adachi, Yoshizawa, & Yagasaki, 2017;Niu et al, 2017), uricase inhibitor and large amounts of purine were administered to develop a mouse model of hyperuricemia. All mice were divided into five groups of 12 mice each and gavaged with the same volume agents for 8 days.…”
Section: Experiments Performed In Oteracil Potassium (Oxo)-induced Hymentioning
“…The cells (1.0 9 10 5 cells/ well) were subcultured into 24-well plates and grown for 72 h in 10% FBS/DMEM/F-12, and then kept for 24 h in serum-free DMEM/F-12. Effect of taxifolin on uric acid production by AML12 cells UA production by AML12 hepatocytes was evaluated as described previously (Adachi et al 2016). In brief, after 24 h culture in serum-free DMEM/F-12, AML12 cells were washed once with phosphate buffered saline without calcium and magnesium [PBS (-)] and then incubated in 200 ll of balanced salt solution (BSS) containing 188 mM NaCl, 5 mM KCl, 1 mM MgCl 2 , 0.8 mM CaCl 2 , 25 mM NaHCO 3 , 1 mM NaH 2 PO 4 , 10 mM HEPES and 5 mM glucose (Petrie et al 2013).…”
Section: Culture Of Aml12 Cellsmentioning
“…On the termination of 2 h incubation, 200 ll of BSS was collected for determination of UA. UA detected in BSS was adopted as an index of its productivity (Adachi et al 2016). As to taxifolin action, the hepatocytes were incubated for 4 h as well as 2 h. After washed once with PBS (-), the hepatocytes were scraped into 300 ll of buffer containing 50 mM Tris (pH 7.5) and 1 mM sodium phosphate, sonicated, and centrifuged (12,0009g, 5 min, 4°C).…”
Section: Culture Of Aml12 Cellsmentioning
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