Assay of topical corticosteroids. Efficacy of suppression of experimental Rhus dermatitis in humans

Abstract

Rhus dermatitis, experimentally induced in humans, was used as a model for determining the efficacy of various proprietary topical steroids. One-centimeter squares of vesicular dermatitis were induced by patch application of Rhus oleoresin. The steroids were then applied without occlusion once daily for four days, with readings of the therapeutic effect taken on the fifth day. Only potent steroids provided unequivocal suppression in this severe test. The rank order of efficacy corresponded to clinical experience. Cream and ointment formulations of the same steroid at the same strength did not differ. High strength preparations were more effective than regular strengths. Relief of pruritus was an important early effect of efficaceous steroids.

B‐Scanning assessment of the anti‐inflammatory activity of methylprednisolone aceponate on experimentally induced allergic reactions

“…This experimental procedure demonstrated that ^-a-fluor-hydrocortisone aeetate has a greater inhibitory effect on eczematous reaetions than hydrocortisone acetate. Kaidbey and Kligman performed pateh testing with Rhus oleoresin on volunteers with a common poison-ivy dermatitis [7]. After 48 h pateh testing, repeated applications of cortieosteroids were performed for 4 consecutive days.…”

“…of the antl-inflammatory effect of these molecules, was first employed by Haxthausen, who induced eczematous reactions in sensitized volunteers [6]. Subsequently, the same test was used for assessing patients who were contact sensitized to various allergens [7][8][9][10][11]. Crijns et al demonstrated a correlation between the blanching seores on normal skin and the anti-inflammatory action on induced contact dermatitis [11].…”

Assessment of Topical Corticosteroid Activity on Experimentally Induced Contact Dermatitis: Echographic Evaluation with Binary Transformation and Image Analysis

“…The first problem was dealt with by Burrows and Stoughton [16], who evaluated the sup pression of the induction of allergy to DNCB by means of preventive application of cortico steroids. Other authors studied the effect of steroids on contact dermatitis induced in sub jects who were already contact-sensitized to nickel, chromium or poison ivy [17][18][19] or on histamine wheals [27], These tests, however, which reproduce the therapeutic action of corticosteroids better than others, were rarely used because of numerous technical problems in performing them, above all because of the difficulty in quantifying slight clinical varia tions induced by different molecules on a der matitis that has already been elicited. These problems have been partially resolved by carrying out both clinical evaluation and in strumental assessment [28],…”

Objective Assessment of Topical Anti-Inflammatory Drug Activity on Experimentally Induced Nickel Contact Dermatitis: Comparison between Visual Scoring, Colorimetry, Laser Doppler Velocimetry and Transepidermal Water Loss

“…A similar method was previously used by Kaidbey and Kligman [7] in 1976 to assess topical corticosteroids: a Rhus dermatitis was induced in hypersensitive human volun teers and the subsequent lesions treated once daily for 4 days. They reported clear-cut results (in terms of visual gradings) only for strong corticosteroids, weak and interme diate-strength steroids not influencing the rate of spontaneous regression after daily applications over 4 days.…”