Assay of the Multiple Energy-Producing Pathways of Mammalian Cells

Abstract

BackgroundTo elucidate metabolic changes that occur in diabetes, obesity, and cancer, it is important to understand cellular energy metabolism pathways and their alterations in various cells.Methodology and Principal FindingsHere we describe a technology for simultaneous assessment of cellular energy metabolism pathways. The technology employs a redox dye chemistry specifically coupled to catabolic energy-producing pathways. Using this colorimetric assay, we show that human cancer cell lines from different organ tissues produce distinct profiles of metabolic activity. Further, we show that murine white and brown adipocyte cell lines produce profiles that are distinct from each other as well as from precursor cells undergoing differentiation.ConclusionsThis technology can be employed as a fundamental tool in genotype-phenotype studies to determine changes in cells from shared lineages due to differentiation or mutation.

Brucella abortus Induces a Warburg Shift in Host Metabolism That Is Linked to Enhanced Intracellular Survival of the Pathogen

“…1 and 2). The host metabolic effects we observed upon B. abortus infection resemble the effect of mitochondrial inhibitors (19) and are consistent with the previously reported downregulation of mitochondrion-associated gene expression in macrophages infected with B. melitensis (20). Many bacteria are known to affect host mitochondria (21), but only a few species have been reported to disrupt mitochondrial structure or localization (9,10,22).…”

Efficient Mitochondrial Glutamine Targeting Prevails Over Glioblastoma Metabolic Plasticity

“…Analysis of various substrates utilization was performed using PM-M1 and PM-M2 Biolog microplates, coated with different oxidizable carbon sources in various wells, following the manufacturer's instructions (18). Briefly, cells (30 Â 10 3 /well) were plated on Biolog microplate for 24 hours in IFM1 media at 37 C and under 5% CO 2 allowing the cells to use up residual carbonenergy sources.…”

Metabolic activity of tree saps of different origin towards cultured human cells in the light of grade correspondence analysis and multiple regression modeling

“…Our in vitro MTS assay demonstrated that tree saps rich in these monosaccharaides facilitated the metabolism of HepG2 cells, whereas sucrose-contacting saps were deprived of this activity. Interestingly, glucose alone, but not sucrose, is able to induce growth of HepG2 cells [28]. Since glucose levels were highly correlated with fructose content across all studied saps, one may expect that fructose also positively affects the growth of HepG2 cells.…”

“…Since glucose levels were highly correlated with fructose content across all studied saps, one may expect that fructose also positively affects the growth of HepG2 cells. In fact, it has been previously demonstrated that HepG2 cells are able to utilize fructose as energy source [28].…”