“…Samples were placed on water baths (Magni Whirl Blue M. Electric Company, USA) at 20.0, 25.0, 30.0, 35.0, 40.0, and 45.0 °C (± 0.1 °C) at least for 48 hours with sporadic stirring in order to achieve the partitioning equilibrium, as it was made studying other compounds (Martínez, Gómez, 2002). After this time-period, the aqueous phase was removed followed by determining the drug concentration by means of UV absorbance measurement and interpolation on a previously constructed calibration curve for KTP in a buffer pH 7.4 (Unicam UV2-100 spectrophotometer, USA for CHL-saturated buffer (Dallos, Liszi, 1995;Baena et al, 2005;Mora et al, 2005).…”