Aspartate aminotransferase Rv3722c governs aspartate-dependent nitrogen metabolism in Mycobacterium tuberculosis

Jansen, Robert S.; Mandyoli, Lungelo; Hughes, Ryan; Wakabayashi, Shoko; Pinkham, Jessica T.; Selbach, Bruna Pelegrim; Guinn, Kristine M.; Rubín, Eric J.; Sacchettini, James C.; Rhee, Kyu Y.

doi:10.1038/s41467-020-15876-8

Cited by 57 publications

(51 citation statements)

References 72 publications

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“…It has been shown that aspartate aminotransferase R3722c mediates an essential role in metabolism and is required for virulence of M.tb ( Jansen et al., 2020 ). Previously, we constructed a filtered PPI network with 441 PPIs between M.tb membrane and secreted proteins and host immunity-related proteins ( Yang et al., 2018 ).…”

Section: Resultsmentioning

confidence: 99%

“…As Rv3722c is essential for M.tb growth in vitro and interacts with host proteins such as TRAF3 (Yang et al, 2018;Jansen et al, 2020), we hypothesized this protein might play an important role Verification of the protein-protein interaction by Y2H assay. Rv3722c in the pmBD destination vector was used as bait.…”

Section: Rv3722c Promotes Mtb Survival In Murine Macrophagesmentioning

confidence: 99%

“…MPT53, an M.tb secreted disulfide-bond-forming-like protein, interacts with TAK1 in a TLR2- or MyD88-independent manner, and induces the host inflammatory responses ( Wang et al., 2019 ). Rv3722c, an M.tb secreted antigen, is annotated as an aspartate aminotransferase, which is a transcription factor that belongs to the GntR family ( Grishin et al., 1995 ; Bramucci et al., 2011 ; Ortega et al., 2016 ; Penn et al., 2018 ; El-Gebali et al., 2019 ; Yang et al., 2019 ; Jansen et al., 2020 ). Proteomics analysis revealed that Rv3722c belongs to the top 10–25% of the most abundant proteins in M.tb ( Wang M, et al., 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…Proteomics analysis revealed that Rv3722c belongs to the top 10–25% of the most abundant proteins in M.tb ( Wang M, et al., 2015 ). Rv3722c was identified to be essential for bacillus growth in vitro and infection ( Griffin et al., 2011 ; Jansen et al., 2020 ). Previously, we identified that Rv3722c can interact with host immune-related proteins in different pathways ( Yang et al., 2018 ), suggesting that Rv3722c may play an important role in disturbing host immune clearance.…”

Section: Introductionmentioning

confidence: 99%

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Rv3722c Promotes Mycobacterium tuberculosis Survival in Macrophages by Interacting With TRAF3

Lei

Cao

et al. 2021

Front. Cell. Infect. Microbiol.

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Mycobacterium tuberculosis (M.tb) secretes numerous proteins to interfere with host immune response for its long-term survival. As one of the top abundant M.tb secreted proteins, Rv3722c was found to be essential for bacilli growth. However, it remains elusive how this protein interferes with the host immune response and regulates M.tb survival. Here, we confirmed that Rv3722c interacted with host TRAF3 to promote M.tb replication in macrophages. Knock-down of TRAF3 attenuated the effect of Rv3722c on the intracellular M.tb survival. The interaction between Rv3722c and TRAF3 hampered MAPK and NF-κB pathways, resulting in a significant increase of IFN-β expression and decrease of IL-1β, IL-6, IL-12p40, and TNF-α expression. Our study revealed that Rv3722c interacted with TRAF3 and interrupted its downstream pathways to promote M.tb survival in macrophages. These findings facilitate further understanding of the mechanism of M.tb secreted proteins in regulating the host cell immune response and promoting its intracellular survival.

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Section: Resultsmentioning

confidence: 99%

Section: Rv3722c Promotes Mtb Survival In Murine Macrophagesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rv3722c Promotes Mycobacterium tuberculosis Survival in Macrophages by Interacting With TRAF3

Lei

Cao

et al. 2021

Front. Cell. Infect. Microbiol.

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“…ABPP can help address this challenge, as ABP-labeling directly signifies protein function (Adam et al, 2004 ; Kamat et al, 2015 ; Xu et al, 2015 ; Martell et al, 2016 ; Ortega et al, 2018 ; Elahi et al, 2019 ). This is especially true when accompanying metabolomics data can provide added confidence (Jansen et al, 2020 ). ABPP can also identify viable whole cells in complex samples based on a certain function without needing any information beforehand, which may aid in the study of un-culturable microbes with poorly characterized genomes.…”

Section: Functional Characterization Of the Gut Microbiome Using Actimentioning

confidence: 99%

Who Is Metabolizing What? Discovering Novel Biomolecules in the Microbiome and the Organisms Who Make Them

Couvillion

Agrawal

Colby

et al. 2020

Front. Cell. Infect. Microbiol.

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Even as the field of microbiome research has made huge strides in mapping microbial community composition in a variety of environments and organisms, explaining the phenotypic influences on the host by microbial taxa—both known and unknown—and their specific functions still remain major challenges. A pressing need is the ability to assign specific functions in terms of enzymes and small molecules to specific taxa or groups of taxa in the community. This knowledge will be crucial for advancing personalized therapies based on the targeted modulation of microbes or metabolites that have predictable outcomes to benefit the human host. This perspective article advocates for the combined use of standards-free metabolomics and activity-based protein profiling strategies to address this gap in functional knowledge in microbiome research via the identification of novel biomolecules and the attribution of their production to specific microbial taxa.

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Synthesis and Performance of Bio‐Based Amphoteric Surfactants

Jansen,

den Bakker,

Venbrux

et al. 2024

Chemistry A European J

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As the global surfactant market continues to expand, there is an increasing need to develop bio‐based alternatives in the shift towards a circular economy. This study focuses on the synthesis of polar, amphoteric, amine‐oxide surfactants starting from biomass‐derived monosaccharides and demonstrating their potential in various applications. The synthesis involved a reductive amination of the sugars with an alkylamine and formaldehyde followed by oxidation to produce N‐oxide surfactants. These bio‐based surfactants exhibited promising properties, including high solubility, foamability, surface tension reduction, and critical micelle concentration. In particular, N‐GalA1.10 and N‐GalA1.12 showed comparable performance to commercial surfactants. Furthermore, these bio‐based surfactants demonstrated significantly lower skin irritation potential when compared to petrochemical‐derived counterparts like sodium laureth sulfate (SLES), making them potentially suitable for personal care products. The biodegradability assessment revealed that N‐GalA1.12 exhibited good biodegradation, indicating its potential environmental compatibility. In conclusion, this study highlights the potential of bio‐based N‐oxide surfactants derived from monosaccharides as sustainable and skin‐friendly alternatives to traditional amphoteric surfactants, like cocamidopropyl betaine (CAPB)

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Aspartate aminotransferase Rv3722c governs aspartate-dependent nitrogen metabolism in Mycobacterium tuberculosis

Cited by 57 publications

References 72 publications

Rv3722c Promotes Mycobacterium tuberculosis Survival in Macrophages by Interacting With TRAF3

Rv3722c Promotes Mycobacterium tuberculosis Survival in Macrophages by Interacting With TRAF3

Who Is Metabolizing What? Discovering Novel Biomolecules in the Microbiome and the Organisms Who Make Them

Synthesis and Performance of Bio‐Based Amphoteric Surfactants

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