Asparagopsis taxiformis decreases enteric methane production from sheep

Li, Xixi; Norman, Hayley C.; Kinley, Robert D.; Laurence, Michael; Wilmot, M. G.; Bender, Hannah; Nys, Rocky de; Tomkins, Nigel

doi:10.1071/an15883

Cited by 159 publications

(150 citation statements)

References 17 publications

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“…A significant reduction in CH 4 production was found when evaluating the effects of A. taxiformis on ruminal fermentation characteristics in-vitro at a 5% OM inclusion rate. Results from the overall experiment show an approximate decrease in TGP by ~50% and in CH 4 production by ~95%, which is similar to the reduction that was reported previously when the effect of A. taxiformis on CH 4 production from beef cattle was investigated [10,18,30] Carbon dioxide production remained similar between the control and A. taxiformis amended vessels. Comparison of total and individual VFA between vessels did not suggest any difference in VFA production at any specific time point with the 5% OM inclusion rate.…”

Section: Discussionsupporting

confidence: 85%

Effect of the macroalgaeAsparagopsis taxiformison methane production and the rumen microbiome assemblage

Roque

Brooke

Ladau

et al. 2018

Preprint

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Background: Recent studies using batch-fermentation suggest that the red macroalgae Asparagopsis taxiformis might reduce methane (CH 4 ) emission from beef cattle by up to ~99% when added to rhodes grass hay, a common feed in the Australian beef industry. These experiments have shown significant reductions in methane without compromising other fermentation parameters (i.e. volatile fatty acid production) with A. taxiformis organic matter (OM) inclusion rates of up to 5%. In the study presented here, A. taxiformis was evaluated for its ability to reduce methane production from dairy cattle fed a mixed ration widely utilized in California; the largest milk producer in the US.Results: Fermentation in a semi-continuous in-vitro rumen system suggests that A. taxiformis can reduce methane production from enteric fermentation in dairy cattle by 95% when added at a 5% OM inclusion rate without any obvious negative impacts on volatile fatty acid production. High-throughput 16S ribosomal RNA (rRNA) gene amplicon sequencing showed that seaweed amendment effects rumen microbiome communities consistent with the Anna Karenina hypothesis, with increased beta-diversity, over time scales of approximately three days. The relative abundance of methanogens in the fermentation vessels amended with A. taxiformis decreased significantly compared to control vessels, but this reduction in methanogen abundance was only significant when averaged over the course of the experiment. Alternatively, significant reductions of methane in the A. taxiformis amended vessels was measured in the early stages of the experiment. This suggests that A. taxiformis has an immediate effect on the metabolic functionality of rumen methanogens whereas its impact on microbiome assemblage, specifically methanogen abundance, is delayed. Conclusions:The methane reducing effect of A. taxiformis during rumen fermentation makes this macroalgae a promising candidate as a biotic methane mitigation strategy in the largest milk producing state in the US. But its effect in-vivo (i.e. in dairy cattle) remains to be investigated in animal trials. Furthermore, to obtain a holistic understanding of the biochemistry responsible for the significant reduction of methane, gene expression profiles of the rumen microbiome and the host animal are warranted.

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Section: Discussionsupporting

confidence: 85%

Effect of the macroalgaeAsparagopsis taxiformison methane production and the rumen microbiome assemblage

Roque

Brooke

Ladau

et al. 2018

Preprint

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“…In the present study, in vivo predicted CH 4 production was inhibited almost completely by AT already at a level of 0.5% on an OM basis. Li et al [45] added AT to diets fed to sheep and observed a reduction of CH 4 production at inclusion levels exceeding 1% of OM intake, but with altered rumen fermentation at all inclusion rates, i.e., at inclusions ≥0.5% of OM intake. On the other hand, in a short-term in vivo experiment by Stefenoni et al [46], inclusion of AT at 0.5% of DM intake decreased CH 4 emission in lactating dairy cows by 80%, with no negative effects on DM intake and milk yield (rumen fermentation parameters were not measured).…”

Section: Dietary Strategies To Decrease Ch 4 Production From Ruminantsmentioning

confidence: 99%

In Vitro Evaluation of Different Dietary Methane Mitigation Strategies

Chagas

Ramin

Krizsan

2019

Animals

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Simple Summary: Dietary methane mitigation strategies do not necessarily make food production from ruminants more energy-efficient, but reducing methane (CH 4 ) in the atmosphere immediately slows down global warming, helping to keep it within 2 • C above the pre-industrial baseline. There is no single most efficient strategy for mitigating enteric CH 4 production from domestic ruminants on forage-based diets. This study assessed a wide variety of dietary CH 4 mitigation strategies in the laboratory, to provide background for future studies with live animals on the efficiency and feasibility of dietary manipulation strategies to reduce CH 4 production. Among different chemical and plant-derived inhibitors and potential CH 4 -reducing diets assessed, inclusion of the natural antimethanogenic macroalga Asparagopsis taxiformis showed the strongest, and dose-dependent, CH 4 mitigating effect, with the least impact on rumen fermentation parameters. Thus, applying Asparagopsis taxiformis at a low daily dose was the best potential dietary mitigation strategy tested, with promising long-term effects, and should be further studied in diets for lactating dairy cows. Abstract:We assessed and ranked different dietary strategies for mitigating methane (CH 4 ) emissions and other fermentation parameters, using an automated gas system in two in vitro experiments. In experiment 1, a wide range of dietary CH 4 mitigation strategies was tested. In experiment 2, the two most promising CH 4 inhibitory compounds from experiment 1 were tested in a dose-response study. In experiment 1, the chemical compounds 2-nitroethanol, nitrate, propynoic acid, p-coumaric acid, bromoform, and Asparagopsis taxiformis (AT) decreased predicted in vivo CH 4 production (1.30, 21.3, 13.9, 24.2, 2.00, and 0.20 mL/g DM, respectively) compared with the control diet (38.7 mL/g DM). The 2-nitroethanol and AT treatments had lower molar proportions of acetate and higher molar proportions of propionate and butyrate compared with the control diet. In experiment 2, predicted in vivo CH 4 production decreased curvilinearly, molar proportions of acetate decreased, and propionate and butyrate proportions increased curvilinearly with increased levels of AT and 2-nitroethanol. Thus 2-nitroethanol and AT were the most efficient strategies to reduce CH 4 emissions in vitro, and AT inclusion additionally showed a strong dose-dependent CH 4 mitigating effect, with the least impact on rumen fermentation parameters.

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“…Methane measurements were taken using seven open circuit respiration chambers as described by Li et al (2016a). CH 4 recovery was made for individual chambers prior to chamber measurement following the procedure of Klein and Wright (2006).…”

Section: Enteric Methane Measurementmentioning

confidence: 99%

The impact of supplementation with Rhagodia preissii and Atriplex nummularia on wool production, mineral balance and enteric methane emissions of Merino sheep

Norman

Hendry

et al. 2017

Grass and Forage Science

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The Australian perennial shrubs, oldman saltbush (Atriplex nummularia) and rhagodia (Rhagodia preissii), can complement the diets of sheep grazing moderate‐quality cereal residues. We compared liveweight change, organic matter digestibility, nitrogen and mineral balance, wool growth and methane emissions of Merino wethers offered oaten hay (OMD 622 g kg−1 DM; N 9.74 g kg−1 DM) with and without shrub biomass (substituted at 25% of OM). Diets were fed at restricted levels for 1 month. Substituting hay with shrub biomass significantly decreased liveweight loss, increased clean wool growth and increased apparent absorption of phosphorus compared to the hay alone. Substitution of hay with both saltbush and rhagodia led to over 23% greater clean wool growth. Nitrogen balance and apparent digestion of organic matter, calcium and magnesium did not vary significantly between animals on the diets. Wethers on the rhagodia and hay diets had similar enteric methane emissions, while animals offered the saltbush diet had significantly higher emissions (L/OM intake). When methane is expressed in terms of wool growth, animals on the rhagodia diet produced 26% less methane for every gram of wool.

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Asparagopsis taxiformis decreases enteric methane production from sheep

Cited by 159 publications

References 17 publications

Effect of the macroalgaeAsparagopsis taxiformison methane production and the rumen microbiome assemblage

Effect of the macroalgaeAsparagopsis taxiformison methane production and the rumen microbiome assemblage

In Vitro Evaluation of Different Dietary Methane Mitigation Strategies

The impact of supplementation with Rhagodia preissii and Atriplex nummularia on wool production, mineral balance and enteric methane emissions of Merino sheep

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