Ascorbate Efflux as a New Strategy for Iron Reduction and Transport in Plants

Grillet, Louis; Ouerdane, Laurent; Flis, Paulina; Hoang, Minh Thi Thanh; Isaure, Marie‐Pierre; Łobiński, Ryszard; Curie, Catherine; Mari, Stéphane

doi:10.1074/jbc.m113.514828

Cited by 150 publications

(133 citation statements)

References 52 publications

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“…Whatever the origin of Fe 2+ , we hypothesize that LPR1-dependent Fe 3+ production in the apoplast initiates Fe redox cycling as a potential source of ROS (Kosman, 2010;Meguro et al, 2007). Effluxed ascorbate may reduce the LPR1 product to redox-active Fe 2+ (Grillet et al, 2014), thereby triggering callose deposition and root cell differentiation. Indeed, LPR1 overexpression causes ectopic Fe 3+ and ROS generation in -Pi ( Figure 5D), and genotype-dependent ROS formation in the apoplast is detectable in Pi-deprived root tips ( Figure 6).…”

Section: Lpr1-dependent Fe Accumulation Controls Callose Deposition Imentioning

confidence: 99%

Iron-Dependent Callose Deposition Adjusts Root Meristem Maintenance to Phosphate Availability

et al. 2015

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Plant root development is informed by numerous edaphic cues. Phosphate (Pi) availability impacts the root system architecture by adjusting meristem activity. However, the sensory mechanisms monitoring external Pi status are elusive. Two functionally interacting Arabidopsis genes, LPR1 (ferroxidase) and PDR2 (P5-type ATPase), are key players in root Pi sensing, which is modified by iron (Fe) availability. We show that the LPR1-PDR2 module facilitates, upon Pi limitation, cell-specific apoplastic Fe and callose deposition in the meristem and elongation zone of primary roots. Expression of cell-wall-targeted LPR1 determines the sites of Fe accumulation as well as callose production, which interferes with symplastic communication in the stem cell niche, as demonstrated by impaired SHORT-ROOT movement. Antagonistic interactions of Pi and Fe availability control primary root growth via meristem-specific callose formation, likely triggered by LPR1-dependent redox signaling. Our results link callose-regulated cell-to-cell signaling in root meristems to the perception of an abiotic cue.

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Section: Lpr1-dependent Fe Accumulation Controls Callose Deposition Imentioning

confidence: 99%

Iron-Dependent Callose Deposition Adjusts Root Meristem Maintenance to Phosphate Availability

et al. 2015

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“…In this case, Fe is present in the apoplast in form of complexes with citrate and malate (Fe(III) 3 Cit 2 Mal 2 , Fe(III) 3 Cit 3 Mal, Fe(III)Cit 2 ) (Roschzttardtz et al 2011;Grillet et al 2013). Interestingly, Grillet et al (2013) showed that before uptake to embryo, Fe(III) was reduced to Fe(II) not enzymatically by ferric reductase, but chemically by ascorbate. Presence of this ascorbate reduction system was shown in two dicotyledonous plants: Pisum sativum and Arabidopsis thaliana (Grillet et al 2013).…”

Section: Iron Traffickingmentioning

confidence: 99%

“…Interestingly, Grillet et al (2013) showed that before uptake to embryo, Fe(III) was reduced to Fe(II) not enzymatically by ferric reductase, but chemically by ascorbate. Presence of this ascorbate reduction system was shown in two dicotyledonous plants: Pisum sativum and Arabidopsis thaliana (Grillet et al 2013). …”

Section: Iron Traffickingmentioning

confidence: 99%

Low-molecular weight organic acids and peptides involved in the long-distance transport of trace metals

Kutrowska

Szeląg

2014

Acta Physiol Plant

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Higher plants evolved mechanisms of uptake, distribution and accumulation of trace metals essential for the proper functioning of the organism (e.g., copper, zinc). Non-essential metals (e.g., cadmium, arsenic, lead) can also enter plant cells using the routes dedicated to the essential ones, because of the shared similar chemical and physical properties. Generally, trace elements are very reactive, able to generate reactive oxygen species and to interact or bind various organic ligands composed of C, H, O, N, P or S. Thus, after entering to the cells, metals are transported and sequestered mainly in a complex form, bound with amino acids, organic acids, peptides or specific metal-binding ligands. Considering diverse properties (e.g., pH value, abundancy of ions, redox state) characterizing cells, tissues and phloem or xylem sap, plants use various ligands to form stable complexes in different conditions. This literature review aims to provide a comprehensive overview on the role of low-molecular weight acids and peptides in trace metals translocation.

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“…It was found that the eﬄux of ASC in pea embryos could increase ferric reduction (Grillet et al, 2014). Similarly, the mutant of ascorbate biosynthesis genes in Arabidopsis ( vtc2-4 , vtc5-1 , and vtc5-2 ), was significantly reduced the ferric reduction activity and Fe content in embryos (Grillet et al, 2014). In the mammalian brain, the acquisition of iron in astrocytes was dependent on the eﬄux of ASC (Lane et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Ascorbate Alleviates Fe Deficiency-Induced Stress in Cotton (Gossypium hirsutum) by Modulating ABA Levels

Guo

Wang

et al. 2017

Front. Plant Sci.

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Fe deficiency causes significant losses to crop productivity and quality. To understand better the mechanisms of plant responses to Fe deficiency, we used an in vitro cotton ovule culture system. We found that Fe deficiency suppressed the development of ovules and fibers, and led to tissue browning. RNA-seq analysis showed that the myo-inositol and galacturonic acid pathways were activated and cytosolic APX (ascorbate peroxidase) was suppressed in Fe-deficient treated fibers, which increased ASC (ascorbate) concentrations to prevent tissue browning. Suppression of cytosolic APX by RNAi in cotton increased ASC contents and delayed tissue browning by maintaining ferric reduction activity under Fe-deficient conditions. Meanwhile, APX RNAi line also exhibited the activation of expression of iron-regulated transporter (IRT1) and ferric reductase–oxidase2 (FRO2) to adapt to Fe deficiency. Abscisic acid (ABA) levels were significantly decreased in Fe-deficient treated ovules and fibers, while the upregulated expression of ABA biosynthesis genes and suppression of ABA degradation genes in Fe-deficient ovules slowed down the decreased of ABA in cytosolic APX suppressed lines to delay the tissue browning. Moreover, the application of ABA in Fe-deficient medium suppressed the development of tissue browning and completely restored the ferric reduction activity. In addition, ABA 8′-hydroxylase gene (GhABAH1) overexpressed cotton has a decreased level of ABA and shows more sensitivity to Fe deficiency. Based on the results, we speculate that ASC could improve the tolerance to Fe deficiency through activating Fe uptake and maintaining ABA levels in cotton ovules and fibers, which in turn reduces symptom formation.

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Ascorbate Efflux as a New Strategy for Iron Reduction and Transport in Plants

Cited by 150 publications

References 52 publications

Iron-Dependent Callose Deposition Adjusts Root Meristem Maintenance to Phosphate Availability

Iron-Dependent Callose Deposition Adjusts Root Meristem Maintenance to Phosphate Availability

Low-molecular weight organic acids and peptides involved in the long-distance transport of trace metals

Ascorbate Alleviates Fe Deficiency-Induced Stress in Cotton (Gossypium hirsutum) by Modulating ABA Levels

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