As bases experimentais da lesão por isquemia e reperfusão do fígado: revisão

Miranda, Luíz Bruner de; Viaro, Fernanda; Ceneviva, Reginaldo; Évora, Paulo Roberto Barbosa

doi:10.1590/s0102-86502004000100001

Cited by 30 publications

(42 citation statements)

References 35 publications

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“…Elevated levels of AST and ALT is observed experimentally in hepatic ischemia and reperfusion 2,14 , which is also observed in group I/R of this research, whereas in the Sham group there was no elevation of these enzymes. In group IPo, levels of AST and ALT were increased with lower intensity, demonstrating the protective effect of this procedure in hepatic ischemia and reperfusion.…”

Section: Discussionsupporting

confidence: 78%

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Postconditioning effect in the hepatic ischemia and reperfusion in rats

Santos¹,

Pontes²,

Miiji

et al. 2010

Acta Cir. Bras.

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Purpose:To evaluate the effect of postconditioning protection in liver tissue of rats submitted to ischemia and reperfusion. Methods: 25 Wistar male rats were randomized in three groups: Group A (ischemia and reperfusion -I/R), with 10 rats, which was made ischemia by vascular clamp application in hepatic hilum for 30 minutes and reperfusion for 60 minutes by removal of the clamp; Group B (Postconditioning -IPo), with 10 rats, with same procedure plus postconditioning (3 cycles of reperfusion inserted by 3 cycles of ischemia, 30 seconds for each phase, between ischemia and reperfusion phases); and Group C (Sham), with 5 rats, which took place only laparotomy and manipulation of the hepatic hilum. Specimens were examined (histological evaluation) and dosage of serum AST and ALT was made. The statistical analysis was made with t Student test, with significant difference when p<0.05. Results: In the Sham group there was no histological or enzymatic changes; In group A the mean tissue lesion was 1.6 and in group B 1.1 (p=0.014). In group A the mean ALT level was 355U/l and in group B was 175.9U/l (p=0.016). The mean of AST was 828.8U/l in group A and 295.5U/l in group B (p=0.001). Conclusion: The postconditioning was able to minimize the severity of liver injury in rats submitted to ischemia and reperfusion. Key words: Ischemia. Reperfusion. Liver. Rats. RESUMO Objetivo:Avaliar o efeito do pós-condicionamento isquêmico na proteção tecidual hepática de ratos submetidos à isquemia e reperfusão. Métodos: Foram utilizados 25 ratos da linhagem Wistar provenientes do Biotério da Universidade Federal de Mato Grosso do Sul, distribuídos em três grupos: A) grupo isquemia/reperfusão (I/R) (10 ratos), em que foi realizada isquemia e reperfusão hepática pela aplicação e retirada de clampe do hilo hepático durante 30 e 60 minutos, respectivamente; B) grupo pós-condicionamento isquêmico (PoCI) (10 ratos), em que foi realizado procedimento semelhante ao anterior, exceto pela realização do pós-condicionamento isquêmico entre as fases de isquemia e reperfusão, consistindo na retirada e colocação do clampe do hilo hepático por 30 segundos cada fase, durante 3 ciclos; grupo C (Sham) (5 ratos), em que realizou-se apenas laparotomia e manipulação do hilo hepático. Foram analisados os espécimes (avaliação histológica), dosagem sorológica de AST e ALT. Resultados: No grupo Sham não se observou alterações histológicas nem enzimáticas; no grupo A a média de lesão tecidual foi 1,6 e no grupo B 1,1 (p=0,014). No grupo A a média de ALT foi 355U/l e no grupo B 175,9U/l (p=0,016). A média de AST foi de 582,8U/l no grupo A e 295,5U/l no grupo B (p=0,001). Conclusão: O pós-condicionamento isquêmico foi capaz de minimizar a intensidade das lesões hepáticas de ratos submetidos à isquemia e reperfusão. Descritores: Isquemia. Reperfusão. Fígado. Ratos.

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Section: Discussionsupporting

confidence: 78%

“…However, this occlusion leads to ischemia followed by reperfusion, which can cause damage to hepatocytes and distance, often worsening the clinical situation of patients 1,2 .…”

Section: Introductionmentioning

confidence: 99%

Postconditioning effect in the hepatic ischemia and reperfusion in rats

Santos¹,

Pontes²,

Miiji

et al. 2010

Acta Cir. Bras.

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“…However, the main side effect of the clamping techniques consists in hepatic ischemia-reperfusion injury, which induces hepatocellular suffering and involves complex metabolic pathways 2 . The ischemia-reperfusion injury consists of changes observed during the reperfusion period, which are mainly related to ischemic time and it includes hepatic microcirculatory alterations, hypotension, elevated levels of serum aminotransferase and LDH , mitochondrial dysfunction and lipid peroxidation 3 . These changes are the result of a complex association between ATP depletion, and leukocyte activation, Kupffer cells in the liver sinusoids, release of proteases and phospholipases, complement activation and formation of reactive oxygen species 3,4 .…”

Section: Introductionmentioning

confidence: 99%

Effect of hyperbaric oxygen therapy on liver function during intermittent ischemia

Baldim

Nejo

Souza³

et al. 2013

Acta Cir. Bras.

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PURPOSE:To analyze the effects of hyperbaric oxygen therapy on liver function in rats previously subjected to ischemia and reperfusion. METHODS: A randomly distribution of 23 Wistar rats was conducted into three groups: SHAM, animals subjected to surgical stress without restricting blood flow by clamping the hepatic pedicle, IR, rats underwent hepatic vascular occlusion intermittently for two complete cycles of 15 minutes of ischemia followed by 5 min of reperfusion, IR / HBO, rats underwent hepatic pedicle clamping and thereafter exposed to hyperbaric oxygen pressure of 2 absolute atmospheres for 60 minutes. We evaluated liver function through mitochondrial function, determined by the stages 3 and 4 of respiration, respiratory control ratio (RCR) and mitochondrial permeability transition (Swelling). Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were also quantified . We analyzed the results using the Mann-Whitney test and were considered significant all results with p <0.05. RESULTS: There were significant differences between the results of stage 3 in SHAM vs IR group ; of the stage 4 in the groups IR vs SHAM and SHAM vs IR /HBO; of the Respiratory Control Ratio (RCR) in the group IR vs IR / HBO ; of alanine aminotransferase in the groups IR vs SHAM , SHAM vs IR/HBO and IR vs IR / HBO; aspartate aminotransferase in the groups SHAM vs IR and SHAM vs IR / HBO. CONCLUSION: The whole analysis of the mitochondiral function indicators permits us to conclude that the hyperbaric oxygen therapy acted as a protective agent of the mitochondrial function, minimizing the ischemia-reperfusion injury of the hepatic parenchyma. Key words: Hyperbaric Oxygen Therapy. Mitochondria. Liver Ischemia. Reperfusion. RESUMO OBJETIVO:Analisar os efeitos da oxigenoterapia hiperbárica sobre a função hepática de ratos previamente submetidos à isquemia e reperfusão. MÉTODOS: Realizou-se a distribuição aleatória de 23 ratos Wistar em três grupos: SHAM, animais submetidos ao estresse cirúrgico sem restrição de fluxo sanguíneo por clampeamento do pedículo hepático; IR ratos submetidos a oclusão vascular hepática de maneira intermitente em dois ciclos completos de 15 minutos de isquemia, seguidos por 5 min de reperfusão; IR/HBO, ratos submetidos ao clampeamento do pedículo hepático e ,ulteriormente , expostos a oxigenoterapia hiperbárica a pressão de 2 atmosferas absolutas durante 60 minutos. Avaliou-se a função hepática através da função mitocondrial, determinada pelos estados 3 e 4 da respiração mitocondrial, razão de controle respiratório(RCR) e transição de permeabilidade mitocondrial (Swelling) .Quantificou-se também alanina aminotransferase e aspartato aminotransferase séricas. Analisou-se os resultados pelo teste de Mann-Whitney e foram considerados significativos os valores de p < 0,05. RESULTADOS:Houve diferença significativa nos valores do estado 3 no grupo SHAM vs IR; estado 4 nos grupos SHAM vs IR, SHAM vsIR/HBO; Razão de Controle Respiratório(RCR) no grupo IR vs IR/HBO; alanina aminotransferase n...

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“…The damage caused by ischemia in the liver is due to microvascular alterations such as increased adhesion and activation of leukocytes, Kupffer cells and platelets, depletion of adenosine triphosphate, complement activation, liberation of calcium and reactive oxygen species formation 6 . The oxidative phosphorylation process is also severely affected by reperfusion, because of its location in the inner mitochondrial membrane, which is highly damaged by the oxygen reactive species.…”

Section: Introductionmentioning

confidence: 99%

Hyperoxic preconditioning in partial liver ischemia

et al. 2014

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PURPOSE:To evaluate the effect of the hyperbaric oxygen (HBO) treatment as a pre-conditioning for I/R effects in the liver ischemia. METHODS:Fifty-seven male Wistar rats (260-300g) were submitted to the following procedures: SHAM; I/R, rats submitted to I/R, consisting of partial ischemia of 70% of the liver for 90 minutes followed by 15 minutes of reperfusion; HBO I/R 1 ATA, 30 minutes of HBO treatment at the pressure of 1 absolute atmosphere (ATA) during the ischemia time. HBO I/R 2 ATA, 30 minutes of HBO (2 ATA) during the ischemia time. Pre HBO I/R 30', rats submitted to 30 minutes of HBO (2 ATA) immediately before the I/R time. Pre HBO I/R 90', rats submitted to 90 minutes of HBO (2 ATA) immediately before the I/R time. RESULTS:There was a significant worsening of all the parameters of mitochondrial energy production (state 3,4, RCR and Swelling) in the I/R group, when compared to the Sham group (I/R

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As bases experimentais da lesão por isquemia e reperfusão do fígado: revisão

Cited by 30 publications

References 35 publications

Postconditioning effect in the hepatic ischemia and reperfusion in rats

Postconditioning effect in the hepatic ischemia and reperfusion in rats

Effect of hyperbaric oxygen therapy on liver function during intermittent ischemia

Hyperoxic preconditioning in partial liver ischemia

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