Artificial Biosensors: How Can Molecular Imprinting Mimic Biorecognition?

Cieplak, Maciej; Kutner, Włodzimierz

doi:10.1016/j.tibtech.2016.05.011

Cited by 197 publications

(120 citation statements)

References 89 publications

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“…Now it's high time to exploit this sequential evolution of protein imprinting to solve 'real' life problems of society. Although MIPs are deeply researched to replace proteins in sensing applications as proteins are highly delicate and labile to slight changes in the surrounding media, but still a huge gap between general lab-scale use and industrial scale applications lies [5]. MIPs have continuously shown capability to replace ELISA kits with imprinted kits [38][39][40].…”

Section: Future Prospectsmentioning

confidence: 99%

“…The main sensing feature of MIPs comprises selective recognition of target analyte because of the dedicated architecture of cavities embedded in the polymer matrix. Cieplak and Kutner state 'MIPs can recognize target analytes not only by their shape and size, because introducing a dedicated set of recognizing sites into the imprinted cavity increases both the affinity of the cavity for the analyte and its selectivity with respect to interferences' [5].…”

Section: Introductionmentioning

confidence: 99%

“…In spite of these advantages and burgeoning research in the field of MIPs, imprinting fraternity [5] still seeks answer of "When will inexpensive, user-friendly, sensitive, and selective (with respect to chosen analytes) devices capable of routine use in clinical analysis, such as for early disease diagnosis, be produced and appear on the market? And is it feasible to design MIPs with selectivity and affinity to an analyte as high as that of natural receptors, including enzymes, antibodies and histones?…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Epitope Imprinting Approach to Monitor Diseases

Singh¹,

Gupta²,

Raghuwanshi³

2017

J Mol Genet Med

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Section: Future Prospectsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Epitope Imprinting Approach to Monitor Diseases

Singh¹,

Gupta²,

Raghuwanshi³

2017

J Mol Genet Med

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“…Within this work, we present first proof-of-concept results for the development of a new method for assessing the blood coagulation status of thrombocytes, through sensors based on molecularly imprinted polymers (MIP) coated onto the electrodes of quartz crystal microbalances (QCM) [4]. The main difficulty in this case lies in the fact that platelet activation during imprinting has to be avoided, which makes this task more challenging than e.g., erythrocyte MIP [5].…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel Platelets Functional Assay Using QCM

Strallhofer

Jung

Jungbauer

et al. 2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

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Thrombocytes and their metabolites are involved in numerous physiological and pathophysiological processes, such as blood coagulation at the site of ruptured endothelium. Normal platelet aggregation can be suppressed via platelet antagonists or non-steroidal anti-inflammatory drugs (NSAIDs). That's why differentiating between resting ("normal") and inhibited platelets in a quick and precise fashion could be of a great clinical significance. Herein, we present the first step on the way to a novel platelet functionality assay based on Molecularly Imprinted Polymers (MIP) coated onto Quartz Crystal Microbalance (QCM). First results reveal sensor responses that depend on platelet concentration and on incubation of platelets with aspirin: acetyl salicylic acid (AAS).

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“…

Molecularly imprinted polymers (MIPs) are excellent example of bio-mimicking recognition materials [1]. They have found numerous applications in selective chemosensing [2].

…”

mentioning

confidence: 99%

Self-Reporting Molecularly Imprinted Polymer for Label-Free Selective Electrochemical Sensing of p-synephrine

Lach

Cieplak

Sharma

et al. 2017

Proceedings of the 5th International Symposium on Sensor Science (I3S 2017)

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Molecularly imprinted polymers (MIPs) are excellent example of bio-mimicking recognition materials [1]. They have found numerous applications in selective chemosensing [2]. For electrochemical determination of electro inactive analytes, usually some external redox probe is added to the sample solutions. It is assumed that binding of target analyte molecules by MIP molecular cavities causes MIP film swelling or shrinking. This behavior leads to changes in MIP film permittivity for the redox probe and thus changes in faradaic currents corresponding to reduction or oxidation of the redox probe (so called "gating effect") in CV and DPV determinations [3,4]. However, this mechanism seems to be inadequate for electrochemical sensors with conductive MIP film recognition units. For example, electrochemical impedance spectroscopy spectra recorded in our previous research [5,6] clearly indicate that redox probe diffusion to the electrode surface was not affected by analite binding into MIP film. Moreover, well pronounced changes in charge transfer resistance were observed. These changes strongly suggest that drop of the redox probe oxidation peak in DPV determination originates from changes in electrochemical properties of the MIP film. Therefore, we can speculate that diffusion of a redox probe is a not crucial issue in terms of selective determination with the MIP film coated electrode. Therefore, a new specially designed monomer, vis., p-bis (2,2'-bithien-5-yl)methyl-ferrocene benzene was used for deposition of a self-reporting MIP film. This monomer acted as both a crosslinking monomer and an internal redox probe. It was electropolymerized together with 2,2'-bitiofen-5-carboxylic acid in the presence of the p-synephrine template-a diet supplement that is suspected of causing serious cardiovascular diseases. These selfreporting MIP film modified electrodes were used for electrochemical determination of p-synephrine in the absence of the external redox probe. For that, appropriate counterions were immobilized within the MIP either by lipophilic chromopropoic acid entrapment inside the MIP matrix, or by copolymerization of thiophene-2-methylsulfonic acid. In both cases, DPV measurements using PBS (pH = 7.4) showed oxidation of ferrocene at ~450 mV vs. Ag/AgCl and a relative change of the DPV peak current was proportional to the concentration of p-synephrine in the range of 10-100 nM with LOD equal to 5 nM.

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Artificial Biosensors: How Can Molecular Imprinting Mimic Biorecognition?

Cited by 197 publications

References 89 publications

Epitope Imprinting Approach to Monitor Diseases

Epitope Imprinting Approach to Monitor Diseases

Development of a Novel Platelets Functional Assay Using QCM

Self-Reporting Molecularly Imprinted Polymer for Label-Free Selective Electrochemical Sensing of p-synephrine

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