Arp2/3 complex subunit ARPC2 binds to microtubules

Havelková, Lenka; Nanda, Gitanjali; Martinek, Jan; Bellinvia, Erica; Sikorová, Lenka; Šlajcherová, Kateřina; Seifertová, Daniela; Fischer, Lukáš; Fišerová, Jindřiška; Petrášek, Jan; Schwarzerová, Kateřina

doi:10.1016/j.plantsci.2015.10.001

Cited by 29 publications

(27 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As reported previously, Arp2/3 complex is essential for cell motility [11, 20]. As one of the subunits of Arp2/3, ARPC2 also promoted tumor development and progression.…”

Section: Discussionsupporting

confidence: 57%

“…The other subunits are ARPC1, ARPC3, ARPC4, and ARPC5 and two actin-related proteins Arp2 and Arp3 [6–9]. In the Arp2/3 complex, ARPC2 subunit holds a central structural position and helps relay both signals and conformational changes [7, 10, 11]. Ghouleh et al demonstrated that ARPC2 participated in promoting smooth muscle cell migration [6].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Role of ARPC2 in Human Gastric Cancer

Zhang

Liu

et al. 2017

Mediators of Inflammation

View full text Add to dashboard Cite

Gastric cancer continues to be the second most frequent cause of cancer deaths worldwide. However, the exact molecular mechanisms are still unclear. Further research to find potential targets for therapy is critical and urgent. In this study, we found that ARPC2 promoted cell proliferation and invasion in the human cancer cell line MKN-28 using a cell total number assay, MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay, cell colony formation assay, migration assay, invasion assay, and wound healing assay. For downstream pathways, CTNND1, EZH2, BCL2L2, CDH2, VIM, and EGFR were upregulated by ARPC2, whereas PTEN, BAK, and CDH1 were downregulated by ARPC2. In a clinical study, we examined the expression of ARPC2 in 110 cases of normal human gastric tissues and 110 cases of human gastric cancer tissues. ARPC2 showed higher expression in gastric cancer tissues than in normal gastric tissues. In the association analysis of 110 gastric cancer tissues, ARPC2 showed significant associations with large tumor size, lymph node invasion, and high tumor stage. In addition, ARPC2-positive patients exhibited lower RFS and OS rates compared with ARPC2-negative patients. We thus identify that ARPC2 plays an aneretic role in human gastric cancer and provided a new target for gastric cancer therapy.

show abstract

“…As reported previously, Arp2/3 complex is essential for cell motility [11, 20]. As one of the subunits of Arp2/3, ARPC2 also promoted tumor development and progression.…”

Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Role of ARPC2 in Human Gastric Cancer

Zhang

Liu

et al. 2017

Mediators of Inflammation

View full text Add to dashboard Cite

show abstract

“…Actin organization and dynamics is also known to be intimately interlinked with that of microtubules (e.g. [30, 62]); it thus remains to be clarified what is the role of the microtubule cytoskeleton in controlling the anisotropic behavior of microfilaments.…”

Section: Discussionmentioning

confidence: 99%

A new kymogram-based method reveals unexpected effects of marker protein expression and spatial anisotropy of cytoskeletal dynamics in plant cell cortex

Cvrčková

Oulehlová

2017

Plant Methods

View full text Add to dashboard Cite

BackgroundCytoskeleton can be observed in live plant cells in situ with high spatial and temporal resolution using a combination of specific fluorescent protein tag expression and advanced microscopy methods such as spinning disc confocal microscopy (SDCM) or variable angle epifluorescence microscopy (VAEM). Existing methods for quantifying cytoskeletal dynamics are often either based on laborious manual structure tracking, or depend on costly commercial software. Current automated methods also do not readily allow separate measurements of structure lifetime, lateral mobility, and spatial anisotropy of these parameters.ResultsWe developed a new freeware-based, operational system-independent semi-manual technique for analyzing VAEM or SDCM data, QuACK (Quantitative Analysis of Cytoskeletal Kymograms), and validated it on data from Arabidopsis thaliana fh1 formin mutants, previously shown by conventional methods to exhibit altered actin and microtubule dynamics compared to the wild type. Besides of confirming the published mutant phenotype, QuACK was used to characterize surprising differential effects of various fluorescent protein tags fused to the Lifeact actin probe on actin dynamics in A. thaliana cotyledon epidermis. In particular, Lifeact-YFP slowed down actin dynamics compared to Lifeact-GFP at marker expression levels causing no macroscopically noticeable phenotypic alterations, although the two fluorophores are nearly identical. We could also demonstrate the expected, but previously undocumented, anisotropy of cytoskeletal dynamics in elongated epidermal cells of A. thaliana petioles and hypocotyls.ConclusionsOur new method for evaluating plant cytoskeletal dynamics has several advantages over existing techniques. It is intuitive, rapid compared to fully manual approaches, based on the free ImageJ software (including macros we provide here for download), and allows measurement of multiple parameters. Our approach was already used to document unexpected differences in actin mobility in transgenic A. thaliana expressing Lifeact fusion proteins with different fluorophores, highlighting the need for cautious interpretation of experimental results, as well as to reveal hitherto uncharacterized anisotropy of cytoskeletal mobility in elongated plant cells.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-017-0171-9) contains supplementary material, which is available to authorized users.

show abstract

“…While in most of higher plants, ARP2/3 coats the surface of biochemically and morphologically distinct organelles, in Arabidopsis , AtARP3 subunit is present in nuclei, the mitochondria, and chloroplasts (Zhang et al, 2013). Within these subcellular sites, AtARPC4 was found to be associated with microsomes and the nucleus (Kotchoni et al, 2009; Zhang et al, 2013), while AtARPC2 was found bound directly to micro-tubulins, and interacted with both actin filaments and microtubules (Havelková et al, 2015). To explain these differences, we hypothesize that one function of the Arp2/3 complex may be to regulate organelle positioning and the patterns of organelle transport as a distinct function of each of the different subunits.…”

Section: Discussionmentioning

confidence: 99%

TaARPC3, Contributes to Wheat Resistance against the Stripe Rust Fungus

Wang

Sun

et al. 2017

Front. Plant. Sci.

View full text Add to dashboard Cite

The actin cytoskeleton participates in numerous cellular processes, including less-characterized processes, such as nuclear organization, chromatin remodeling, transcription, and signal transduction. As a key regulator of actin cytoskeletal dynamics, the actin related protein 2/3 complex (Arp2/3 complex) controls multiple developmental processes in a variety of tissues and cell types. To date, the role of the Arp2/3 complex in plant disease resistance signaling is largely unknown. Herein, we identified and characterized wheat ARPC3, TaARPC3, which encodes the C3 subunit of the Arp2/3 complex. Expression of TaARPC3 in the arc18 mutant of Saccharomyces cerevisiae Δarc18 resulted in complementation of stress-induced phenotypes in S. cerevisiae, as well as restore wild-type cell shape malformations. TaARPC3 was found predominantly to be localized in the nucleus and cytoplasm when expressed transiently in wheat protoplast. TaARPC3 was significantly induced in response to avirulent race of Puccinia striiformis f. sp. tritici (Pst). Knock-down of TaARPC3 by virus-induced gene silencing resulted in a reduction of resistance against Pst through a specific reduction in actin cytoskeletal organization. Interestingly, this reduction was found to coincide with a block in reactive oxygen species (ROS) accumulation, the hypersensitive response (HR), an increase in TaCAT1 mRNA accumulation, and the growth of Pst. Taken together, these findings suggest that TaARPC3 is a key subunit of the Arp2/3 complex which is required for wheat resistance against Pst, a process that is associated with the regulation of the actin cytoskeleton.

show abstract

Arp2/3 complex subunit ARPC2 binds to microtubules

Cited by 29 publications

References 65 publications

Role of ARPC2 in Human Gastric Cancer

Role of ARPC2 in Human Gastric Cancer

A new kymogram-based method reveals unexpected effects of marker protein expression and spatial anisotropy of cytoskeletal dynamics in plant cell cortex

TaARPC3, Contributes to Wheat Resistance against the Stripe Rust Fungus

Contact Info

Product

Resources

About