Arp2/3 Complex and Actin Depolymerizing Factor/Cofilin in Dendritic Organization and Treadmilling of Actin Filament Array in Lamellipodia

Svitkina, Tatyana; Borisy, Gary G.

doi:10.1083/jcb.145.5.1009

Cited by 1,043 publications

(1,007 citation statements)

References 70 publications

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“…This includes the leading edge of Lamellipodia [25] and the cleavage furrow in dividing cells [26], [27]. These previous findings are consistent with our results.…”

Section: Discussionsupporting

confidence: 93%

The distribution of cofilin and DNase I in vivo

2002

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Actin is the principal component of the cytoskeleton, a structure that can be disassembled and reassembled in a matter of seconds in vivo. The state of assembly of actin in vivo is primarily regulated by one or more actin binding proteins (ABPs). Typically, the actions of ABPs have been studied one by one, however, we propose that multiple ABPs, acting cooperatively, may be involved in the control of actin filament length. Cofilin and DNase I are two ABPs that have previously been demonstrated to form a ternary complex with actin in vitro. This is the first report to demonstrate their co-localisation in vivo, and differences in their distributions. Our observations strongly suggest a physiological role for higher order complexes of actin in regulation of cytoskeletal assembly during processes such as cell division.

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“…This includes the leading edge of Lamellipodia [25] and the cleavage furrow in dividing cells [26], [27]. These previous findings are consistent with our results.…”

Section: Discussionsupporting

confidence: 93%

The distribution of cofilin and DNase I in vivo

2002

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“…2002), and the ARP2/3 complex is known to promote the formation of actin networks by initiating the polymerization of new actin filaments around already existing actin (Mullins et al. 1998; Svitkina and Borisy 1999). …”

Section: Discussionmentioning

confidence: 99%

Adaptional evolution of trichome in Caragana korshinskii to natural drought stress on the Loess Plateau, China

Ning

Wang

Zhou

et al. 2016

Ecology and Evolution

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Caragana korshinskii is commonly employed to improve drought ecosystems on the Loess Plateau, although the molecular mechanism at work is poorly understood, particularly in terms of the plant's ability to tolerate drought stress. Water is the most severe limiting factor for plant growth on the Loess Plateau. The trichome is known to play an efficient role in reducing water loss through decreasing the rate of transpiration, so in this study, we focused on the trichome‐related gene expression of ecological adaptation in C. korshinskii under low precipitation conditions. In order to explore the responses of trichomes to drought, we selected two experimental sites from wet to dry along the Loess Plateau latitude gradient for observation. Micro‐phenomena through which trichomes grew denser and larger under reduced precipitation were observed using a scanning electron microscope; de novo transcriptomes and quantitative PCR were then used to explore and verify gene expression patterns of C. korshinskii trichomes. Results showed that GIS2,TTG1, and GL2 were upregulated (as key positive‐regulated genes on trichome development), while CPC was downregulated (negative‐regulated gene). Taken together, our data indicate that downstream genes of gibberellin and cytokinin signaling pathways, alongside several cytoskeleton‐related genes, contribute to modulating trichome development to enhance transpiration resistance ability and increase the resistance to drought stress in C. korshinskii.

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“…Protrusion-Protrusion of the cell leading edge is the actual start of the cell migration cycle. The mechanical force for protrusion is provided by polymerization of actin filaments at the leading edge, either as long parallel bundles to form filopodia, or as a branched dendritic network during lamellipodia formation (Pollard and Borisy, 2003;Ponti et al, 2004;Ridley et al, 2003;Svitkina and Borisy, 1999;Vicente-Manzanares et al, 2005). In both cases, addition of actin subunits onto the fast-growing 'barbed' ends of the actin filaments physically pushes the membrane forward in the direction of migration.…”

Section: Basic Principles Of Cell Migration Overall Structure Of the mentioning

confidence: 99%

Cell biology of embryonic migration

Kurosaka¹,

Kashina²

2008

Birth Defects Research Pt C

113

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Cell migration is an evolutionarily conserved mechanism that underlies the development and functioning of uni-and multicellular organisms and takes place in normal and pathogenic processes, including various events of embryogenesis, wound healing, immune response, cancer metastases, and angiogenesis. Despite the differences in the cell types that take part in different migratory events, it is believed that all of these migrations occur by similar molecular mechanisms, whose major components have been functionally conserved in evolution and whose perturbation leads to severe developmental defects. These mechanisms involve intricate cytoskeleton-based molecular machines that can sense the environment, respond to signals, and modulate the entire cell behavior. A big question that has concerned the researchers for decades relates to the coordination of cell migration in situ and its relation to the intracellular aspects of the cell migratory mechanisms. Traditionally, this question has been addressed by researchers that considered the intra-and extracellular mechanisms driving migration in separate sets of studies. As more data accumulate researchers are now able to integrate all of the available information and consider the intracellular mechanisms of cell migration in the context of the developing organisms that contain additional levels of complexity provided by extracellular regulation. This review provides a broad summary of the existing and emerging data in the cell and developmental biology fields regarding cell migration during development.

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Arp2/3 Complex and Actin Depolymerizing Factor/Cofilin in Dendritic Organization and Treadmilling of Actin Filament Array in Lamellipodia

Cited by 1,043 publications

References 70 publications

The distribution of cofilin and DNase I in vivo

The distribution of cofilin and DNase I in vivo

Adaptional evolution of trichome in Caragana korshinskii to natural drought stress on the Loess Plateau, China

Cell biology of embryonic migration

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