Aromatic compounds from the endophytic fungus Asordaria conoidea and their allelochemical property using OSMAC strategy

Abstract

Endophytic fungi are biodiverse and alternative source of bioactive compounds, due their different abilities of genetic expression and alteration of biosynthetic pathway when submitted to different culture conditions. The metabolic profile of three different crude extracts (A, B and C), obtained from the endophytic fungus Asordaria conoidea, were evaluated by HPLC and 1 H NMR. Antioxidant and allelochemical activity were also evaluated. OSMAC diversified the metabolic production, mainly in the solid culture, where the tyrosol, 4-hydroxybenzaldehyde, 2-phenylacetamide and vanillic acid were isolated. The structures of the compounds were elucidated mainly by NMR. Extracts had antioxidant potential, however, only Extract C showed allelochemical activity, as inhibition of 65.5% in growth. This study confirms the efficiency of the OSMAC platform in producing extracts of different properties and compounds. Herein the A. conoidea was isolated for the first time as an endophytic microorganism.

Phenolic compounds and bioactive extract produced by endophytic fungus Coriolopsis rigida

“…The endophytic fungus isolated from C. regium was named Cr-1 until a taxonomic name could be assigned. The fungus was acquired from the fungi collection of the Federal University of Tocantins (Gurupi Campus), and it was identified using the CTAB (cationic hexadecyl trimethyl ammonium bromide) extraction protocol adapted from Venkateswarulu et al (Venkateswarulu et al 2018;Arruda et al 2021), which utilized molecular analyses of the genomic DNA extracted from the fungal biomass, obtained after fifteen days of cultivation in Potato Dextrose Broth (PDB). The DNA was amplified using the Internal Transcribed Spacer Region (ITS)-1 and ITS-4 primers in pre-programmed thermocycler (Techne TC-5000), and the amplified product was purified using the Purilink PCR Purification Kit (Invitrogen).…”

Caracterização Química De Extratos Brutos Obtidos De Dois Fungos Endofíticos Isolados De Cochlospermum Regium

“…Compostos aromáticos apresentam características antioxidantes (Andrade, 2018;Sousa, 2007), vários fungos endofíticos já foram relatados contendo atividade antioxidantes (Alves et al, 2020) devido a sua produção de compostos aromáticos (Arruda et al, 2021, Chapla et al, 2013. Esta propriedade pode estar associada a proteção do hospedeiro de fatores abióticos, como o composto 3 que tem propriedade de filtrar raios ultravioletas (UV-B, 280-320nm) e já foi detectado em plantas nas em regiões superficiais da estrutura vegetal (Azevedo, 1998;Gobbo-Neto, 2007;Ferreira, 2008).…”

Extracellular enzymes produced by Coriolopsis rigida an endophytic fungus associated with Cochlospermum regium

“…The endophytic fungus C. regium was acquired from the fungi collection of the Federal University of Tocantins (Gurupi Campus), and it was identified using the CTAB (cationic hexadecyl trimethyl ammonium bromide) extraction protocol adapted from Venkateswarulu et al (Venkateswarulu et al, 2018;Arruda et al, 2021). The molecular analyses was performed using the genomic DNA extracted from the fungal biomass, obtained after fifteen days of cultivation in Potato Dextrose Broth (PDB).…”

“…Cochlospermum regium (Bixaceae) is a medicinal plant, native from Brazil, the roots are used in the preparation of decoctions, infusions and bottles for the treatment of uterine, intestinal and ovarian inflammations (Camilo et al, 2016). However, few studies from the microbiota of this plant are described (Arruda et al, 2021, Montel et al, 2021.…”