AroC, a chorismate synthase, is required for the formation of Edwardsiella tarda biofilms

Li, Rui; Gao, Daqing; Fang, Zhengzou; Zhao, Lei; Xu, Zeyan; Qin, Chuyu; Zhang, Ruolan; Xu, Jinfeng; Lu, Chenyang

doi:10.1016/j.micinf.2022.104955

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…These include aroC and aroE , which are involved in the synthesis of aromatic amino acids via the shikimate pathway. AroC has previously been demonstrated to affect biofilm formation in Escherichia coli , Streptococcus mutans and Edwardsiella tarda , and is essential for the production of intermediates, such as indole, that affect flagellar synthesis and biofilm formation [ 83–85 ]. AroE has also previously been found to be associated with biofilm adaptation in L. monocytogenes [ 86 ].…”

Section: Resultsmentioning

confidence: 99%

Contrasting genes conferring short- and long-term biofilm adaptation in Listeria

Monteith,

Pascoe,

Mourkas

et al. 2023

Microbial Genomics

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Listeria monocytogenes is an opportunistic food-borne bacterium that is capable of infecting humans with high rates of hospitalization and mortality. Natural populations are genotypically and phenotypically variable, with some lineages being responsible for most human infections. The success of L. monocytogenes is linked to its capacity to persist on food and in the environment. Biofilms are an important feature that allow these bacteria to persist and infect humans, so understanding the genetic basis of biofilm formation is key to understanding transmission. We sought to investigate the biofilm-forming ability of L. monocytogenes by identifying genetic variation that underlies biofilm formation in natural populations using genome-wide association studies (GWAS). Changes in gene expression of specific strains during biofilm formation were then investigated using RNA sequencing (RNA-seq). Genetic variation associated with enhanced biofilm formation was identified in 273 genes by GWAS and differential expression in 220 genes by RNA-seq. Statistical analyses show that the number of overlapping genes flagged by either type of experiment is less than expected by random sampling. This novel finding is consistent with an evolutionary scenario where rapid adaptation is driven by variation in gene expression of pioneer genes, and this is followed by slower adaptation driven by nucleotide changes within the core genome.

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Section: Resultsmentioning

confidence: 99%

Contrasting genes conferring short- and long-term biofilm adaptation in Listeria

Monteith,

Pascoe,

Mourkas

et al. 2023

Microbial Genomics

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show abstract

“…These include aroC and aroE, which are involved in the synthesis of aromatic amino acids via the shikimate pathway. AroC has previously been demonstrated to affect biofilm formation in Escherichia coli, Streptococcus mutans and Edwardsiella tarda, and is essential for the production of intermediates, such as indole, that affect flagellar synthesis and biofilm formation (Liu et al, 2022;Puttamreddy, Cornick and Minion, 2010;Shemesh, Tam and Steinberg, 2007). AroE has also previously been found to be associated with biofilm adaptation in L. monocytogenes (Santos et al, 2019).…”

Section: Genome-wide Association Study Identifies Genetic Elements As...mentioning

confidence: 98%

Contrasting genes conferring short and long-term biofilm adaptation inListeria

Monteith

Pascoe

Mourkas

et al. 2023

Preprint

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Listeria monocytogenes is an opportunistic food-borne bacterium that is capable of infecting humans with high rates of hospitalisation and mortality. Natural populations are genotypically and phenotypically variable, with some lineages being responsible for most human infections. The success of L. monocytogenes is linked to its capacity to persist on food and in the environment. Biofilms are an important feature that allow these bacteria to persist and infect humans, therefore, understanding the genetic basis of biofilm formation is key to understanding transmission. We sought to investigate the biofilm forming ability of L. monocytogenes by identifying genetic variation that underlies biofilm formation in natural populations using genome-wide association studies. Changes in gene expression of specific strains during biofilm formation were then investigated using RNAseq. Genetic variation associated with enhanced biofilm formation was identified in 273 genes by GWAS and differential expression in 220 genes by RNAseq. Statistical analyses show that number of overlapping genes flagged by either type of experiment is less than expected by random sampling. This is consistent with an evolutionary scenario where rapid adaptation is driven by variation in gene expression of pioneer genes, and this is followed by slower adaptation driven by nucleotide changes within the core genome.

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“…The main mechanism of action known for glyphosate is the inhibition of the shikimate pathway. This pathway is crucial for the biosynthesis of aromatic metabolites, some of which are known to be important for biofilm formation in certain microbes (43)(44)(45). However, whether shikimate pathway inhibition by glyphosate is associated with biofilm disruption is unknown.…”

Section: Introductionmentioning

confidence: 99%

Glyphosate Effects on Growth and Biofilm Formation in Bee Gut Symbionts and Diverse Associated Bacteria

Motta,

de Jong,

Gage

et al. 2024

Preprint

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Biofilm formation is a common adaptation enabling bacteria to thrive in various environments and to withstand external pressures. In the context of host-microbe interactions, biofilms play vital roles in establishing microbiomes associated with animals and plants and are used by opportunistic microbes to facilitate proliferation within hosts. Investigating biofilm dynamics, composition, and responses to environmental stressors is crucial for understanding microbial community assembly and biofilm regulation in health and disease. In this study, we explore the independent gut colonization and in vitro biofilm formation abilities of core members of the honey bee (Apis mellifera) gut microbiota. Additionally, we assess the impact of glyphosate, a widely used herbicide with antimicrobial properties, and a glyphosate-based formulation on growth and biofilm formation in bee gut symbionts as well as in other biofilm-forming bacteria associated with diverse animals and plants. Our results demonstrate that several strains of core bee gut bacterial species can independently colonize the bee gut, which probably depends on their ability to form biofilms. Furthermore, glyphosate exposure has varying effects on bacterial growth and biofilm formation. These findings imply specific impacts of environmental stressors on microbial biofilms with both ecological and host health-related implications.

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AroC, a chorismate synthase, is required for the formation of Edwardsiella tarda biofilms

Cited by 6 publications

References 37 publications

Contrasting genes conferring short- and long-term biofilm adaptation in Listeria

Contrasting genes conferring short- and long-term biofilm adaptation in Listeria

Contrasting genes conferring short and long-term biofilm adaptation inListeria

Glyphosate Effects on Growth and Biofilm Formation in Bee Gut Symbionts and Diverse Associated Bacteria

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