Are the glandular trichomes in Jacquinia armillaris (Theophrastoideae—Primulaceae) salt glands?

Luna, Bruna Nunes de; Freitas, Maria de Fátima; Silva, Karla Marins Mattos da; Barros, Cláudia Franca

doi:10.1007/s00709-019-01472-1

Cited by 2 publications

(3 citation statements)

References 41 publications

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“…In our protoplast pools, E12 and E9 (defined as PGTs and other GTs, respectively) only had 45 and 96 cells, respectively, whereas the leaf trichome cells of Arabidopsis were lacking in scRNA-seq data because trichome cells were somewhat resistant to protoplasting ( Zhang et al., 2021b ). The trichome cells are covered with plenty of cutin and wax, creating a hurdle for single-cell preparation ( de Luna et al., 2020 ; Fich et al., 2020 ; Konarska and Lotocka, 2020 ). Extraction of the nucleus and single nucleus RNA sequencing (snRNA-seq) may provide solutions for the above problems to capture different cell types regardless of cell size ( Habib et al., 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes

et al. 2022

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The peltate glandular trichomes (PGTs) on Nepeta tenuifolia leaves can secrete and store bioactive essential oils. ScRNA-seq is a powerful tool for uncovering heterogeneous cells and exploring the development and differentiation of specific cells. Due to leaves rich in PGTs, the young leaves were used to isolated protoplasts and successfully captured 33,254 protoplasts for sequencing purposes. After cell type annotation, all the cells were partitioned into six broad populations with 19 clusters. Cells from PGTs were identified based on the expression patterns of trichome-specific genes, monoterpene biosynthetic genes, and metabolic analysis of PGT secretions. The developmental trajectories of PGTs were delineated by pseudotime analysis. Integrative analysis of scRNA-seq data from N. tenuifolia leaves and Arabidopsis thaliana shoot revealed that PGTs were specific to N. tenuifolia. Thus, our results provide a promising basis for exploring cell development and differentiation in plants, especially glandular trichome initiation and development.

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Section: Discussionmentioning

confidence: 99%

Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes

et al. 2022

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“…These traits could be correlated with a water-saving process via intensive accumulation of Na + in leaves which consequently resulted in a marked decrease in water loss rate through down-regulation of stomatal aperture size and decrease in density [44]. Glandular trichomes observed in J. armillaris are adapted to salt secretion, characterizing as salt glands that participate in sodium and others ion excretion [20,21]. Na + and Cl − ion exclusion is one of the most vital phenomena to enable high salt-tolerance in plants [46].…”

Section: Discussionmentioning

confidence: 99%

“…In this way, it is expected that a species that possesses such phenotypic plasticity as J. armillaris (still growing in similar latitudes) might vary its anatomy in response to differences in the characteristics of the island's soil (more saline and with less humidity) and due to the rainfall amount (slightly higher in Turpialito). Previous work [17,18] suggests that specific structures such as stomatal and trichomes could play an important role in reducing the impact of salinity; Even, recently trichomes have been confirmed as salt excretory glands [20,21]. So, it was hypothesized that leaf anatomical and structural traits associated with drought and salinity tolerance would correspond with a habitat.…”

Section: Introductionmentioning

confidence: 99%

Leaf morphoanatomical traits of Jacquinia armillaris Jacq. (Theophrastoideae - Primulaceae) in two xeric shrublands from Venezuela

Galviz

Valerio

2021

Neotropical Biodiversity

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This study aimed to investigate the leaf anatomy of Jacquinia armillaris plants in two different Venezuelan xeric shrublands to evaluate possible variations caused by the environments, which differ in rainfall and soil salinity. Leaf samples were collected in two sites: La Tortuga Island, a dryer and salty environment than the other collecting site, Turpialito, a coastal location in the mainland. The epidermis and the mesophylls were observed with a light microscope and measured with an ocular micrometer inserted in one of the eyepieces. The results show that J. armillaris has many characteristic anatomical traits of xerophytes, among them: thick cuticles and thick epidermis in both leaf surfaces, stomata only in the abaxial side and epidermal depressions lodging a glandular trichome. Leaves are bifacial and present multistratified palisade parenchyma facing the adaxial epidermis and abaxial spongy parenchyma with abundant intercellular spaces. Furthermore, the hypodermis is composed of one or two cell layers below the upper epidermis. Leaf lamina dimension, petiole, cuticles, epidermis and hypodermis cells, as well as the stomatal occlusive cells and number of trichomes, had differences in size and number when the plants of both sites were compared, being higher in plants from La Tortuga. On the other hand, palisade parenchyma and stomatal density were higher in Turpialito plants. Plants collected in La Tortuga Island showed characteristics that suggest adaptation to the island's saline and more arid conditions in comparison to plants from Turpialito, which presented more leaf traits related to adaptations to water deficit. These results demonstrate the phenotypic plasticity of J. armillaris plants that grow in two different xeric shrublands.

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Are the glandular trichomes in Jacquinia armillaris (Theophrastoideae—Primulaceae) salt glands?

Cited by 2 publications

References 41 publications

Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes

Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes

Leaf morphoanatomical traits of Jacquinia armillaris Jacq. (Theophrastoideae - Primulaceae) in two xeric shrublands from Venezuela

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