Are resin-containing pulp capping materials as reliable as traditional ones in terms of local and systemic biological effects?

Bakir, Elif Pınar; Yıldırım, Zehra; Bakır, Salih; Ketani, Aydın

doi:10.4012/dmj.2021-065

Cited by 12 publications

(11 citation statements)

References 29 publications

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“…These results are in accordance with previous studies on several cell subpopulations (Saygili et al, 2017; Rodríguez‐Lozano et al, 2019). The resinous component in endodontic sealers or in resin‐modified calcium silicate cements has been associated with the increased cytotoxicity (Bakir et al, 2022; Sanz, Soler‐Doria, et al, 2021; Silva et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

et al. 2022

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Aim To assess the cytocompatibility and bioactive potential of the new calcium silicate cement‐based sealer AH Plus Bioceramic Sealer (AHPbcs) on human periodontal ligament stem cells (hPDLSCs) compared with the epoxy resin‐based sealer AH Plus (AHP) and the calcium silicate cement‐based sealer Endosequence BC Sealer (ESbcs). Methodology Standardized sample discs and 1:1, 1:2 and 1:4 eluates of the tested materials were prepared. The following assays were performed: surface element distribution via SEM–EDX, cell attachment and morphology via SEM, cell viability via a MTT assay, cell migration/proliferation via a wound‐healing assay, osteo/cemento/odontogenic marker expression via RT‐qPCR and cell mineralized nodule formation via Alizarin Red S staining. HPDLSCs were isolated from extracted third molars. Comparisons were made with hPDLSCs cultured in unconditioned (negative control) or osteogenic (positive control) culture media. Statistical significance was established at p < .05. Results A higher peak of Ca2+ was detected from ESbcs compared with AHPbcs and AHP in SEM–EDX. Both AHPbcs and ESbcs showed significantly positive results in the cytocompatibility assays (cell viability, migration/proliferation, attachment and morphology) compared with a negative control group, whilst AHP showed significant negative results. Both AHPbcs and ESbcs exhibited an upregulation of at least one osteo/odonto/cementogenic marker compared with the negative and positive control groups. Both ESbcs and AHPbcs showed a significantly higher calcified nodule formation than the negative and positive control groups, indicative of their biomineralization potential and were also significantly higher than AHP group. Conclusion AH Plus Bioceramic Sealer exhibited a significantly higher cytocompatibility and bioactive potential than AH Plus and a similar cytocompatibility to that of Endosequence BC Sealer. Endosequence BC Sealer exhibited a significantly higher mineralization potential than the other tested sealers. The results from this in vitro study act as supporting evidence for the use of AH Plus Bioceramic Sealer in root canal treatment.

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Section: Discussionmentioning

confidence: 99%

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

et al. 2022

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“…Even though ACTIVA BioACTIVE supports human dental pulp stem cells’ (hDPSCs) proliferation, mineralization and attachment, further evaluation of its cytotoxicity needs to be conducted before being used for VPT [ 28 ]. Based on available research, it can be stated that, in cases of resin-containing materials, the risk of severe inflammation is higher than with CSMs; however, ACTIVA BioACTIVE-BASE/LINER showed some promising results in terms of more successful local and systemic tissue responses [ 29 ]. Literature does not provide sufficient evidence to substantiate its use in VPT; available data suggest that resin-free hydraulic calcium-silicate cements promote cell viability and bioactivity towards human cells better than resin-based agents, resulting in more successful clinical outcomes [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

The Cytotoxicity and Genotoxicity of Bioactive Dental Materials

Kunert

Rozpędek‐Kamińska

Galita

et al. 2022

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The promotion of biologically based treatment strategies in restorative dentistry is of paramount importance, as invasive treatments should be avoided to maintain the tooth’s vitality. This study aimed to assess the biocompatibility of commercially available bioactive materials that can be used for dental pulp capping. The study was performed with a monocyte/macrophage peripheral blood SC cell line (ATCC CRL-9855) on the following six specific bioactive materials: ProRoot MTA (Dentsply Sirona), MTA Angelus (Angelus), Biodentine (Septodont), TheraCal LC (Bisco), ACTIVA BioACTIVE (Pulpdent) and Predicta Bioactive Bulk (Parkell). The cytotoxicity of the investigated agents was measured using a resazurin-based cell viability assay, while the genotoxicity was evaluated using an alkaline comet assay. Additionally, flow cytometry (FC) apoptosis detection was conducted with a FITC (fluorescein isothiocyanate) Annexin V Apoptosis Detection Kit I. FC cell-cycle arrest assessment was carried out with propidium iodide staining. The results of this study showed no significant cytotoxicity and genotoxicity (p > 0.05) in ProRoot MTA, MTA Angelus, Biodentine, ACTIVA BioACTIVE and Predicta Bioactive. Conversely, TheraCal LC presented a significant decrease (p < 0.001). In conclusion, due to excellent biocompatibility and low cytotoxicity, MTA, Biodentine, ACTIVA BioACTIVE and Predicta Bioactive may be suitable for pulp capping treatments. On the other hand, due to the high cytotoxicity of TheraCal LC, its use should be avoided in vital pulp therapies.

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“…Concerning the microbiological effect, a randomized clinical study was conducted using permanent carious molars treated with indirect capping and found that the reduction of the bacterial count in both Streptococcus mutans and Lactobacillus was using Biner LC (light-cured hydroxy calcium phosphate) or TheraCal LC [ 38 ]. Moreover, in vivo subcutaneous implantation test performed in rats showed irregular collagen formation and tissue inflammation; such study informed that inflammation in the liver and kidney tissue of the rats was more severe with the use of TheraCal LC, possibly attributable to the light-cured compounds [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Clinical Efficacy of Biomimetic Bioactive Biomaterials for Dental Pulp Capping: A Systematic Review and Meta-Analysis

et al. 2022

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Recently, biomimetic bioactive biomaterials have been introduced to the market for dental pulp capping. This systematic review and meta-analysis aimed to determine any variation between the effect of using TheraCal LC and other bioactive biomaterials for pulp capping is different, as measured by dentin increment and clinical success. The risk of bias was assessed using the Risk of Bias 2 and Newcastle–Ottawa tools for randomized clinical trials and observational studies. A search for relevant articles was performed on five databases. Additionally, the quality of the included studies was assessed using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) criteria. A summary of individual studies and a meta-analysis were performed. The odds ratio of data from clinical success was combined using a random-effects meta-analysis. The meta-analysis results showed homogeneity between the studies (I2 = 0%). They revealed that the clinical success showed no differences between the patients who received TheraCal LC, light-cured calcium silicate-based biomimetic biomaterial, for dental pulp capping or the comparator biomaterials (p > 0.5). However, the certainty of the evidence was low to moderate due to the risk of bias in the included studies.

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Are resin-containing pulp capping materials as reliable as traditional ones in terms of local and systemic biological effects?

Cited by 12 publications

References 29 publications

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

The Cytotoxicity and Genotoxicity of Bioactive Dental Materials

Clinical Efficacy of Biomimetic Bioactive Biomaterials for Dental Pulp Capping: A Systematic Review and Meta-Analysis

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