Are chitosan natural polymers suitable as adjuvant/delivery system for anti-tuberculosis vaccines?

Khademi, Farzad; Taheri, Ramezan Ali; Avarvand, Arshid Yousefi; Vaez, Hamid; Momtazi‐Borojeni, Amir Abbas; Soleimanpour, Saman

doi:10.1016/j.micpath.2018.05.035

Cited by 50 publications

(30 citation statements)

References 35 publications

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“…This polymer has shown the ability to open tight junctions when applied to a mucous membrane. Furthermore, chitosan has shown increased permeation through the skin when administered transcutaneously [ 51 , 52 ]. Additionally, the positive superficial charge of CSNCs given by chitosan could be another factor that further increases the penetration of OVA through the skin [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Lower-Sized Chitosan Nanocapsules for Transcutaneous Antigen Delivery

2018

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Transcutaneous vaccination has several advantages including having a noninvasive route and needle-free administration; nonetheless developing an effective transdermal formulation has not been an easy task because skin physiology, particularly the stratum corneum, does not allow antigen penetration. Size is a crucial parameter for successful active molecule administration through the skin. Here we report a new core-shell structure rationally developed for transcutaneous antigen delivery. The resulting multifunctional carrier has an oily core with immune adjuvant properties and a polymeric corona made of chitosan. This system has a size of around 100 nm and a positive zeta potential. The new formulation is stable in storage and physiological conditions. Ovalbumin (OVA) was used as the antigen model and the developed nanocapsules show high association efficiency (75%). Chitosan nanocapsules have high interaction with the immune system which was demonstrated by complement activation and also did not affect cell viability in the macrophage cell line. Finally, ex vivo studies using a pig skin model show that OVA associated to the chitosan nanocapsules developed in this study penetrated and were retained better than OVA in solution. Thus, the physicochemical properties and their adequate characteristics make this carrier an excellent platform for transcutaneous antigen delivery.

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Section: Resultsmentioning

confidence: 99%

Lower-Sized Chitosan Nanocapsules for Transcutaneous Antigen Delivery

2018

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“…Chitosan and HA worked together to achieve the activity increase. Chitosan, serving as a type of scaffold and macromolecule carrier, has obviously sustained-release property [29]. Drugs can freely diffuse from the chitosan scaffold or even can be released due to the degradation of chitosan [30].…”

Section: Resultsmentioning

confidence: 99%

Identification of a Novel Anticancer Oligopeptide from Perilla frutescens (L.) Britt. and Its Enhanced Anticancer Effect by Targeted Nanoparticles In Vitro

Riya

et al. 2018

International Journal of Polymer Science

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Objective. Perilla frutescens (L.) Brittis is a dietary herbal medicine and has anticancer effect. However, little is known about its anticancer peptides. This study is aimed at identifying cytotoxic oligopeptides which are loaded by a drug delivery system, to explore its anticancer application. Methods. The oligopeptides were isolated from enzymatic hydrolysates of Perilla seed crude protein by using ultrafiltration, gel filtration chromatography, and reversed-phase high-performance liquid chromatography (RP-HPLC). The structure of the oligopeptide was determined using a peptide sequencer, and its anticancer effect was examined by the MTT assay. PSO (Perilla seed oligopeptide), the most potent anticancer oligopeptide, was loaded by chitosan nanoparticles (NPs) modified by hyaluronic acid (HA). Then, the particle size, zeta potential, encapsulation efficiency (EE), drug loading efficiency (LE), the cumulative release rates of NPs, and its cytotoxic effect on cancer cells were investigated. Results. Three fractions were isolated by the chromatography assay. The third fraction has a broad-spectrum and the strongest anticancer effect. This fraction was further purified and identified as SGPVGLW with a molecular weight of 715 Da and named as PSO. Then, PSO was loaded by HA-conjugated chitosan to prepare HA/PSO/C NPs, which had a uniform size of 216.7 nm, a zeta potential of 35.4 mV, an EE of 38.7%, and an LE of 24.3%. HA/PSO/C NPs had a slow release rate in vitro, with cumulative release reaching to 81.1%. Compared with free PSO, HA/PSO/C NPs showed notably enhanced cytotoxicity and had the strongest potency to human glioma cell line U251. Conclusion. This study demonstrated that PSO, a novel oligopeptide from Perilla seeds, has a broad-spectrum anticancer effect and could be encapsulated by NPs, which enhanced tumor targeting cytotoxicity with obvious controlled release. Our study indicates that Perilla seeds are valuable for anticancer peptide development.

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“…Chitosan, a deacetylated form of chitin composed of D-glucosamine and N-acetyl-D-glucosamine, is a hydrophilic linear polysaccharide polymer with high potential in the delivery of various hydrophilic molecules (e.g., protein) and drug agents (e.g., nano-and microparticles) (27)(28)(29) and immunostimulatory abilities to promote immune responses (19,30). In addition, chitosan is highly suitable for use in medical and pharmaceutical applications because it is biodegradable, biocompatible, nonallergenic, and nontoxic (31).…”

Section: Discussionmentioning

confidence: 99%

The Combinations Chitosan-Pam ₃ CSK ₄ and Chitosan-Monophosphoryl Lipid A: Promising Immune-Enhancing Adjuvants for Anticaries Vaccine PAc

et al. 2019

Infect Immun

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We previously demonstrated that recombinant protein PAc could be administered as an anticaries vaccine. However, the relatively weak immunogenicity of PAc limits its application. In the present study, we investigated the effect of two adjuvant combinations of chitosan plus Pam3CSK4 (chitosan-Pam3CSK4) and of chitosan plus monophosphoryl lipid A (chitosan-MPL) in the immune responses to the PAc protein in vivo and in vitro. PAc-chitosan-Pam3CSK4 or PAc-chitosan-MPL promoted significantly higher PAc-specific antibody titers in serum and saliva, inhibited Streptococcus mutans colonization onto the tooth surfaces, and endowed better protection effect with significantly less caries activities than PAc alone. Chitosan-Pam3CSK4 and chitosan-MPL showed no statistically significant differences. In conclusion, our study demonstrated that the chitosan-Pam3CSK4 and chitosan-MPL combinations are promising for anticaries vaccine development.

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Are chitosan natural polymers suitable as adjuvant/delivery system for anti-tuberculosis vaccines?

Cited by 50 publications

References 35 publications

Lower-Sized Chitosan Nanocapsules for Transcutaneous Antigen Delivery

Lower-Sized Chitosan Nanocapsules for Transcutaneous Antigen Delivery

Identification of a Novel Anticancer Oligopeptide from Perilla frutescens (L.) Britt. and Its Enhanced Anticancer Effect by Targeted Nanoparticles In Vitro

The Combinations Chitosan-Pam ₃ CSK ₄ and Chitosan-Monophosphoryl Lipid A: Promising Immune-Enhancing Adjuvants for Anticaries Vaccine PAc

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Are chitosan natural polymers suitable as adjuvant/delivery system for anti-tuberculosis vaccines?

Cited by 50 publications

References 35 publications

Lower-Sized Chitosan Nanocapsules for Transcutaneous Antigen Delivery

Lower-Sized Chitosan Nanocapsules for Transcutaneous Antigen Delivery

Identification of a Novel Anticancer Oligopeptide from Perilla frutescens (L.) Britt. and Its Enhanced Anticancer Effect by Targeted Nanoparticles In Vitro

The Combinations Chitosan-Pam 3 CSK 4 and Chitosan-Monophosphoryl Lipid A: Promising Immune-Enhancing Adjuvants for Anticaries Vaccine PAc

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The Combinations Chitosan-Pam ₃ CSK ₄ and Chitosan-Monophosphoryl Lipid A: Promising Immune-Enhancing Adjuvants for Anticaries Vaccine PAc