Architecture, Fabrication, and Properties of Stretchable Micro-Electrode Arrays

Tsay, Candice; Lacour, Stéphanie P.; Wagner, S.; Morrison, Barclay

doi:10.1109/icsens.2005.1597913

Cited by 10 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10 μm diameter Polyimide Various (Rodger, 2006) 150 μm diameter Parylene Spinal cord and Retina (Maghribi et al, 2002) 300−350 μm diameter PDMS Retina (Schuettler and Stieglitz, 2000) 500 μm diameter Polyimide-PDMS hybrid Radial Nerve (Sahin et al, 1997) 500μm by 1mm rectangular PDMS Peripheral Nerves (Tsay, 2005) 100μm to 1 mm squares PDMS Hippocampal Culture (Schuettler et al, 2005) 600 μm square PDMS Various…”

mentioning

confidence: 99%

A lithographically-patterned, elastic multi-electrode array for surface stimulation of the spinal cord

Meacham

Giuly

Guo

et al. 2007

Biomed Microdevices

101

View full text Add to dashboard Cite

A new, scalable process for microfabrication of a silicone-based, elastic multi-electrode array (MEA) is presented. The device is constructed by spinning poly(dimethylsiloxane) (PDMS) silicone elastomer onto a glass slide, depositing and patterning gold to construct wires and electrodes, spinning on a second PDMS layer, and then micropatterning the second PDMS layer to expose electrode contacts. The micropatterning of PDMS involves a custom reactive ion etch (RIE) process that preserves the underlying gold thin film. Once completed, the device can be removed from the glass slide for conformal interfacing with neural tissue. Prototype MEAs feature electrodes smaller than those known to be reported on silicone substrate (60 μm diameter exposed electrode area) and were capable of selectively stimulating the surface of the in vitro isolated spinal cord of the juvenile rat. Stretchable serpentine traces were also incorporated into the functional PDMS-based MEA, and their implementation and testing is described.

show abstract

mentioning

confidence: 99%

A lithographically-patterned, elastic multi-electrode array for surface stimulation of the spinal cord

Meacham

Giuly

Guo

et al. 2007

Biomed Microdevices

101

View full text Add to dashboard Cite

show abstract

“…The recording sites were 100µm×100µm square with an inter-electrode spacing of 300µm. The fabrication process has been described in detail in previous work [1,8].…”

Section: A Traumatic Brain Injury Modelmentioning

confidence: 99%

Stretchable microelectrode arrays a tool for discovering mechanisms of functional deficits underlying traumatic brain injury and interfacing neurons with neuroprosthetics

Tsay²,

Lacour³

et al. 2006

2006 International Conference of the IEEE Engineering in Medicine and Biology Society

View full text Add to dashboard Cite

Traumatic brain injury (TBI) can be caused by motor vehicle accidents, falls and firearms. TBI can result in major neurological dysfunction such as chronic seizures and memory disturbances. To discover mechanisms of functional deficits underlying TBI, we developed a stretchable microelectrode array (SMEA),which can be used for continuous recording of neuronal function, pre-, during, and post-stretch injury. TheSMEA was fabricated on a polydimethylsiloxane (PDMS)substrate with stretchable, 100 pm wide, 25 nm thick gold electrodes patterned there on [1]. The electrodes were encapsulated with a 10-20 microm thick, photo-patternable PDMS insulation layer. Previous biocompatibility tests showed no overt necrosis or cell death caused by the SMEAs after 2 weeks in culture [2]. The electrical performance of the SMEAs was tested in electrophysiological saline solution before, during and after biaxial stretching. The results showed that the electrode impedance increased with the strain to reach 800 kL at 8.5% strain and then recovered to 10 kil after relaxation. The working noise level remained below 20 pV pp during the whole process. New methodologiesf or improving the patterning of the encapsulation layer were tested on gold electrode arrays supported on glass. With these prototype arrays, robust population spikes were recorded from organotypic hippocampal slice cultures of brain tissue. Additionally, seizure-like activity induced with 1 mM bicuculline was also recorded. Our results demonstrate that the prototype arrays have good electrical performance compatible with existing multielectrode array systems. They also indicate the ability to record neuronal activity from hippocampal slices. This novel technology will enable new studies to understand injury mechanisms leading to post-traumatic neuronal dysfunction.

show abstract

“…Design, fabrication, and packaging of SMEAs have been described previously in detail. [37][38][39] Briefly, thin-film conductors (3 nm of chromium, followed by 75 nm of gold, finished with 3 nm of chromium) were sequentially deposited on a 280-lm-thick layer of polydimethylsiloxane (PDMS; Sylgard 184; Dow Corning, Midland, MI) by electron beam evaporation. 40 The gold thin-film was patterned into recording electrodes and encapsulated with a 15lm-thick layer of either PDMS or photo-patternable silicone (WL5150; Dow Corning).…”

Section: Stretchable Microelectrode Arraysmentioning

confidence: 99%

Alterations in Hippocampal Network Activity afterIn VitroTraumatic Brain Injury

Kang

Cao

Graudejus

et al. 2015

Journal of Neurotrauma

View full text Add to dashboard Cite

Traumatic brain injury (TBI) alters function and behavior, which can be characterized by changes in electrophysiological function in vitro. A common cognitive deficit after mild-to-moderate TBI is disruption of persistent working memory, of which the in vitro correlate is long-lasting, neuronal network synchronization that can be induced pharmacologically by the gamma-aminobutyric acid A antagonist, bicuculline. We utilized a novel in vitro platform for TBI research, the stretchable microelectrode array (SMEA), to investigate the effects of TBI on bicuculline-induced, long-lasting network synchronization in the hippocampus. Mechanical stimulation significantly disrupted bicuculline-induced, long-lasting network synchronization 24 h after injury, despite the continued ability of the injured neurons to fire, as revealed by a significant increase in the normalized spontaneous event rate in the dentate gyrus (DG) and CA1. A second challenge with bicuculline 24 h after the first challenge significantly decreased the normalized spontaneous event rate in the DG. In addition, we illustrate the utility of the SMEA for TBI research by combining multiple experimental paradigms in one platform, which has the potential to enable novel investigations into the mechanisms responsible for functional consequences of TBI and speed the rate of drug discovery.

show abstract

Architecture, Fabrication, and Properties of Stretchable Micro-Electrode Arrays

Cited by 10 publications

References 17 publications

A lithographically-patterned, elastic multi-electrode array for surface stimulation of the spinal cord

A lithographically-patterned, elastic multi-electrode array for surface stimulation of the spinal cord

Stretchable microelectrode arrays a tool for discovering mechanisms of functional deficits underlying traumatic brain injury and interfacing neurons with neuroprosthetics

Alterations in Hippocampal Network Activity afterIn VitroTraumatic Brain Injury

Contact Info

Product

Resources

About