Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms

Seneviratne, Chaminda Jayampath; Silva, Wander José da; Li, Jin; Samaranayake, Y. H.; Samaranayake, LP

doi:10.1016/j.archoralbio.2009.08.002

Cited by 101 publications

(104 citation statements)

References 31 publications

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“…In agreement with others researches, mature biofilms of C. albicans consisted of a dense network of yeast and hyphal elements (Chandra et al 2001, Ramage et al 2001, Seneviratne et al 2009). Mature biofilms of C. parapsilosis are structurally similar to those described by Kuhn et al (2002).…”

Section: Discussionsupporting

confidence: 92%

Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals

Pires

Santos

Zaia

et al. 2011

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 92%

Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals

Pires

Santos

Zaia

et al. 2011

Mem. Inst. Oswaldo Cruz

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“…7 In addition, biofilm formation and coaggregation effects allow competing microorganisms to maximize the colonization surface area in an environment that is highly competitive for space and nutrients. 23 To date, most previous studies have focused on analyzing biofilm formation by counting CFU/mL; however, Seneviratne et al 10 suggest that biofilm development results should be analyzed by more than one method. In this study, in addition to colony counting, we used an XTT reduction assay that permits measurement of the metabolic activity of the microbial eukaryotic population present in biofilms.…”

Section: Discussionmentioning

confidence: 99%

“…9 The single biofilms formed by C. albicans, C. tropicalis, C. glabrata, and C. krusei, on flat-bottom 96-well microtiter plates for 48 h, showed similar metabolic activity, thus confirming the CFU counting method. Seneviratne et al 10 evaluated the growth kinetics of Candida biofilms by counting CFU after 1.5, 24, 48 and 72 h of development. At an adherence time of 1.5 h, approximately 1-7 x 10 5 cells/mL had adhered to the well of the microtiter plate.…”

Section: Discussionmentioning

confidence: 99%

“…The biofilm formation of Candida was performed as described by Seneviratne et al, 10 with some modifications. Candida albicans biofilm was formed by adding 200 µL from a standardized suspension (10 7 cells/mL) to each well of flat-bottom 96-well microtiter plates (Corning Corporation, Corning, USA).…”

Section: In Vitro Formation Of Simple and Mixed Biofilmsmentioning

confidence: 99%

“…8,9 In general, biofilm development evolves according to four sequential steps: first, adhesion of microorganisms to a surface; second, discrete colony formation and organization of cells; third, secretion of extracellular polysaccharides (EPS) and maturation into a three-dimensional structure; and, fourth, dissemination of progeny biofilm cells. 10 The extracellular matrix of the biofilm can protect the cells from phagocytosis, maintain the integrity of the biofilm, and limit the diffusion of substances. 11 It has been estimated that over 60% of microbial infections are involved with biofilm.…”

Section: Introductionmentioning

confidence: 99%

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Mixed biofilms formed by C. albicans and non-albicans species: a study of microbial interactions

et al. 2016

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Most Candida infections are related to microbial biofilms often formed by the association of different species. The objective of this study was to evaluate the interactions between Candida albicans and non-albicans species in biofilms formed in vitro. The nonalbicans species studied were: Candida tropicalis, Candida glabrata and Candida krusei. Single and mixed biofilms (formed by clinical isolates of C. albicans and non-albicans species) were developed from standardized suspensions of each strain (10 7 cells/mL), on flat-bottom 96-well microtiter plates for 48 hour. These biofilms were analyzed by counting colony-forming units (CFU/mL) in Candida HiChrome agar and by determining cell viability, using the XTT 2,3-bis (2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide colorimetric assay. The results for both the CFU/mL count and the XTT colorimetric assay showed that all the species studied were capable of forming high levels of in vitro biofilm. The number of CFU/mL and the metabolic activity of C. albicans were reduced in mixed biofilms with non-albicans species, as compared with a single C. albicans biofilm. Among the species tested, C. krusei exerted the highest inhibitory action against C. albicans. In conclusion, C. albicans established antagonistic interactions with non-albicans Candida species in mixed biofilms.

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A Membrane‐Targeted Photosensitizer Prevents Drug Resistance and Induces Immune Response in Treating Candidiasis

Wu,

Xu,

et al. 2023

Advanced Science

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Candida albicans (C. albicans), a ubiquitous polymorphic fungus in humans, causes different types of candidiasis, including oral candidiasis (OC) and vulvovaginal candidiasis (VVC), which are physically and mentally concerning and financially costly. Thus, developing alternative antifungals that prevent drug resistance and induce immunity to eliminate Candida biofilms is crucial. Herein, a novel membrane‐targeted aggregation‐induced emission (AIE) photosensitizer (PS), TBTCP‐QY, is developed for highly efficient photodynamic therapy (PDT) of candidiasis. TBTCP‐QY has a high molar absorption coefficient and an excellent ability to generate 1O2 and •OH, entering the interior of biofilms due to its high permeability. Furthermore, TBTCP‐QY can efficiently inhibit biofilm formation by suppressing the expression of genes related to the adhesion (ALS3, EAP1, and HWP1), invasion (SAP1 and SAP2), and drug resistance (MDR1) of C. albicans, which is also advantageous for eliminating potential fungal resistance to treat clinical infectious diseases. TBTCP‐QY‐mediated PDT efficiently targets OC and VVC in vivo in a mouse model, induces immune response, relieves inflammation, and accelerates the healing of mucosal defects to combat infections caused by clinically isolated fluconazole‐resistant strains. Moreover, TBTCP‐QY demonstrates excellent biocompatibility, suggesting its potential applications in the clinical treatment of OC and VVC.

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Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms

Cited by 101 publications

References 31 publications

Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals

Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals

Mixed biofilms formed by C. albicans and non-albicans species: a study of microbial interactions

A Membrane‐Targeted Photosensitizer Prevents Drug Resistance and Induces Immune Response in Treating Candidiasis

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