Arabidopsis UMAMIT24 and 25 are amino acid exporters involved in seed loading

Besnard, Julien; Zhao, Changhao; Avice, Jean‐Christophe; Vitha, Stanislav; Hyodo, Ayumi; Pilot, Guillaume; Okumoto, Sakiko

doi:10.1093/jxb/ery302

Cited by 44 publications

(49 citation statements)

References 66 publications

(94 reference statements)

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“…Amino acids import into and export out of the vacuole requires the activity of transporters. Several amino acid transporters belonging to different families have been found to be localized on the tonoplast [56,73,76]. AtAVT3 family members are homologs of AtANT1 but localized to the vacuolar membrane in Arabidopsis [76].…”

Section: Intracellular Translocation Of Amino Acidsmentioning

confidence: 99%

“…Repression of its expression has no impact on seed set, but seed volume is significantly decreased [ 14 ]. The tonoplast-localized AtUMAMIT24 was found mainly in the chalazal seed coat and may be involved in temporary storage of amino acids in chalaza [ 73 ]. AtUMAMIT25 is targeted to endosperm cells, indicating its role in amino acid export from the endosperm [ 73 ].…”

Section: Amino Acid Transport Processes Mediated By Amino Acid Tramentioning

confidence: 99%

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Amino Acid Transporters in Plant Cells: A Brief Review

Yang

Wei

Huang

et al. 2020

Plants

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Amino acids are not only a nitrogen source that can be directly absorbed by plants, but also the major transport form of organic nitrogen in plants. A large number of amino acid transporters have been identified in different plant species. Despite belonging to different families, these amino acid transporters usually exhibit some general features, such as broad expression pattern and substrate selectivity. This review mainly focuses on transporters involved in amino acid uptake, phloem loading and unloading, xylem-phloem transfer, import into seed and intracellular transport in plants. We summarize the other physiological roles mediated by amino acid transporters, including development regulation, abiotic stress tolerance and defense response. Finally, we discuss the potential applications of amino acid transporters for crop genetic improvement.

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Section: Intracellular Translocation Of Amino Acidsmentioning

confidence: 99%

Section: Amino Acid Transport Processes Mediated By Amino Acid Tramentioning

confidence: 99%

Amino Acid Transporters in Plant Cells: A Brief Review

Yang

Wei

Huang

et al. 2020

Plants

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“…Though the vascular trace of the funiculus terminates at the funiculus‐CZSC interface in Arabidopsis , the vascular system penetrates the CZSC in B. napus (Millar et al ). Furthermore, transport linking the maternal and filial regions have also been reported in Arabidopsis and genes associated with the transport of amino acids (Besnard et al ) and phosphates (Vogiatzaki et al ) have been shown to preferentially accumulate in the chalazal end of the seed. Thus, the chalazal pole of B. napus may function as a gateway for nutrient unloading and seed filling thereby guiding developmental processes.…”

Section: Introductionmentioning

confidence: 97%

Transcriptome landscape of the early Brassica napus seed

Ziegler

Khan

Kalichuk

et al. 2019

JIPB

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Brassica napus L. (canola) is one of the world's most economically important oilseeds. Despite our growing knowledge of Brassica genetics, we still know little about the genes and gene regulatory networks underlying early seed development. In this work, we use laser microdissection coupled with RNA sequencing to profile gene activity of both the maternal and filial subregions of the globular seed. We find subregions of the chalazal end including the chalazal endosperm, chalazal proliferating tissue, and chalazal seed coat, have unique transcriptome profiles associated with hormone biosynthesis and polysaccharide metabolism. We confirm that the chalazal seed coat is uniquely enriched for sucrose biosynthesis and transport, and that the chalazal endosperm may function as an important regulator of the maternal region through brassinosteroid synthesis. The chalazal proliferating tissue, a poorly understood subregion, was specifically enriched in transcripts associated with megasporogenesis and trehalose biosynthesis, suggesting this ephemeral structure plays an important role in both sporophytic development and carbon nutrient balance, respectively. Finally, compartmentalization of transcription factors and their regulatory circuits has uncovered previously unknown roles for the chalazal pole in early seed development.

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“…Previous studies have shown that the AAP family members play critical roles in plant growth, organ development, and abiotic and biotic stress responses [4][5][6][7]. However, there have been few systematic studies on AAPs in B. napus so far.…”

Section: Discussionmentioning

confidence: 99%

“…AAs derived from senescent leaves are the major N forms for seed N nutrients [3]. Therefore, efficient uptake and translocation of AAs are favorable for yield production, and also favorable for plant resistance against various stresses [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of the amino acid permease genes and molecular characterization of their transcriptional responses to various nutrient stresses in allotetraploid rapeseed

et al. 2020

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Background: Nitrogen (N), referred to as a "life element", is a macronutrient essential for optimal plant growth and yield production. Amino acid (AA) permease (AAP) genes play pivotal roles in root import, long-distance translocation, remobilization of organic amide-N from source organs to sinks, and other environmental stress responses. However, few systematic analyses of AAPs have been reported in Brassica napus so far. Results: In this study, we identified a total of 34 full-length AAP genes representing eight subgroups (AAP1-8) from the allotetraploid rapeseed genome (A n A n C n C n , 2n = 4x = 38). Great differences in the homolog number among the BnaAAP subgroups might indicate their significant differential roles in the growth and development of rapeseed plants. The BnaAAPs were phylogenetically divided into three evolutionary clades, and the members in the same subgroups had similar physiochemical characteristics, gene/protein structures, and conserved AA transport motifs. Darwin's evolutionary analysis suggested that BnaAAPs were subjected to strong purifying selection pressure. Ciselement analysis showed potential differential transcriptional regulation of AAPs between the model Arabidopsis and B. napus. Differential expression of BnaAAPs under nitrate limitation, ammonium excess, phosphate shortage, boron deficiency, cadmium toxicity, and salt stress conditions indicated their potential involvement in diverse nutrient stress responses. Conclusions: The genome-wide identification of BnaAAPs will provide a comprehensive insight into their family evolution and AAP-mediated AA transport under diverse abiotic stresses. The molecular characterization of core AAPs can provide elite gene resources and contribute to the genetic improvement of crop stress resistance through the modulation of AA transport.

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Arabidopsis UMAMIT24 and 25 are amino acid exporters involved in seed loading

Abstract: UMAMIT24 and UMAMIT25 are expressed in distinct seed tissue during Arabidopsis embryogenesis, and are both involved in amino acid transfer to the seed.

Cited by 44 publications

References 66 publications

Amino Acid Transporters in Plant Cells: A Brief Review

Amino Acid Transporters in Plant Cells: A Brief Review

Transcriptome landscape of the early Brassica napus seed

Genome-wide identification of the amino acid permease genes and molecular characterization of their transcriptional responses to various nutrient stresses in allotetraploid rapeseed

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