Arabidopsis plants constitutively overexpressing a myo-inositol 1-phosphate synthase gene (SaINO1) from the halophyte smooth cordgrass exhibits enhanced level of tolerance to salt stress

Joshi, Rohit; Ramanarao, Mangu Venkata; Baisakh, Niranjan

doi:10.1016/j.plaphy.2013.01.009

Cited by 53 publications

(33 citation statements)

References 21 publications

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“…PCC6803), which significantly increases the salt tolerance of Escherichia coli and Schizosaccharomyces pombe (61). Furthermore, MIPS overexpression in several species of vascular plants significantly increases the salinity tolerance of these multicellular organisms, suggesting that the MIB pathway is a key biochemical determinant of euryhalinity across all major taxa (62)(63)(64)(65)(66). MIPS overexpression even increases pathogen resistance in sweet potato (67), as is consistent with the critical roles of compatible osmolytes as general cytoprotectants (58,59).…”

Section: Discussionsupporting

confidence: 63%

Osmolality/salinity-responsive enhancers (OSREs) control induction of osmoprotective genes in euryhaline fish

Wang

Kültz

2017

Proc. Natl. Acad. Sci. U.S.A.

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Fish respond to salinity stress by transcriptional induction of many genes, but the mechanism of their osmotic regulation is unknown. We developed a reporter assay using cells derived from the brain of the tilapia Oreochromis mossambicus (OmB cells) to identify osmolality/salinity-responsive enhancers (OSREs) in the genes of O. mossambicus. Genomic DNA comprising the regulatory regions of two strongly salinity-induced genes, inositol monophosphatase 1 (IMPA1.1) and myo-inositol phosphate synthase (MIPS), was isolated and analyzed with dual luciferase enhancer trap reporter assays. We identified five sequences (two in IMPA1.1 and three in MIPS) that share a common consensus element (DDKGGAAWWDWWYDNRB), which we named "OSRE1." Additional OSREs that were less effective in conferring salinity-induced trans-activation and do not match the OSRE1 consensus also were identified in both MIPS and IMPA1.1. Although OSRE1 shares homology with the mammalian osmotic-response element/tonicity-responsive enhancer (ORE/TonE) enhancer, the latter is insufficient to confer osmotic induction in fish. Like other enhancers, OSRE1 trans-activates genes independent of orientation. We conclude that OSRE1 is a cis-regulatory element (CRE) that enhances the hyperosmotic induction of osmoregulated genes in fish. Our study also shows that tailored reporter assays developed for OmB cells facilitate the identification of CREs in fish genomes. Knowledge of the OSRE1 motif allows affinity-purification of the corresponding transcription factor and computational approaches for enhancer screening of fish genomes. Moreover, our study enables targeted inactivation of OSRE1 enhancers, a method superior to gene knockout for functional characterization because it confines impairment of gene function to a specific context (salinity stress) and eliminates pitfalls of constitutive gene knockouts (embryonic lethality, developmental compensation). biochemical evolution | compatible osmolytes | osmotic stress signaling | enhancer | CRE

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Section: Discussionsupporting

confidence: 63%

Osmolality/salinity-responsive enhancers (OSREs) control induction of osmoprotective genes in euryhaline fish

Wang

Kültz

2017

Proc. Natl. Acad. Sci. U.S.A.

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“…However, ferritin and myo-inositol-1-phosphate synthase were commonly up-regulated by both drought and salt stresses. In a previous study, transgenic Arabidopsis plant overexpressing myo-inositol 1-phosphate synthase showed improved growth under salt stress by protecting the photosystem II (Joshi et al, 2013). Ferritins have also shown to be necessary for rapid remodeling of the photosynthetic apparatus, and modulation of ferritin expression is essential for the plant to establish a balance between availability of iron for metabolism to avoid subsequent ROS-related damages (Ravet et al, 2009).…”

Section: Identification Of Drought or Salt-responsive Genesmentioning

confidence: 97%

Response to drought and salt stress in leaves of poplar ( Populus alba × Populus glandulosa ): Expression profiling by oligonucleotide microarray analysis

Yoon

Park

Choi

et al. 2014

Plant Physiology and Biochemistry

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“…The expression of IMT genes seems to be stress up-regulated (Vernon and Bohnert 1992;Sengupta et al 2008). Several studies, conducted on transgenic plants expressing MIPS or IMT genes (and accumulating elevated amounts of myo-inositol or d-ononitol/d-pinitol, respectively), confirm the protective role of cyclitols under stress conditions (Sheveleva et al 1997;Sengupta et al 2008;Patra et al 2010;Joshi et al 2013;Kaur et al 2013). Cyclitols can function as "compatible solutes" (Valluru and Van den Ende 2011), osmolytes (Matos et al 2010) and antioxidants (scavenging of hydroxyl radicals, Nishizawa et al 2008).…”

Section: Discussionmentioning

confidence: 79%

The occurrence and accumulation of d-pinitol in fenugreek (Trigonella foenum graecum L.)

et al. 2018

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This article presents changes in concentrations of d-pinitol (and other cyclitols as well as low molecular weight carbohydrates) in vegetative and reproductive organs of fenugreek (Trigonella foenumgraecum L.) during an entire plant growing period. d-Pinitol was the major cyclitol in all tested organs, representing 43-94% of total cyclitols and 2-77% of total soluble carbohydrates. The highest concentration of d-pinitol was found in pods (14-23 mg g −1 of dry weight, DW), lower in leaves and stems (5-20 and 9-10 mg g −1 DW, respectively), and the lowest in maturing seeds (2-5 mg g −1 DW). Although maturing seeds accumulate α-d-galactosides of d-pinitol (galactosyl pinitols, up to 6.6 mg g −1 DW), the major storage sugars were raffinose family oligosaccharides (RFOs, 65.37 mg g −1 DW). Both RFOs and galactosyl pinitols are hydrolyzed during seed germination, releasing sucrose and d-pinitol, respectively. Accumulation of free galactose was not detected. Owing to the high concentration of d-pinitol (up to 23.70 mg g −1 DW) and low concentration of soluble sugars, developing pods seem to be the best source of d-pinitol.

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Arabidopsis plants constitutively overexpressing a myo-inositol 1-phosphate synthase gene (SaINO1) from the halophyte smooth cordgrass exhibits enhanced level of tolerance to salt stress

Cited by 53 publications

References 21 publications

Osmolality/salinity-responsive enhancers (OSREs) control induction of osmoprotective genes in euryhaline fish

Osmolality/salinity-responsive enhancers (OSREs) control induction of osmoprotective genes in euryhaline fish

Response to drought and salt stress in leaves of poplar ( Populus alba × Populus glandulosa ): Expression profiling by oligonucleotide microarray analysis

The occurrence and accumulation of d-pinitol in fenugreek (Trigonella foenum graecum L.)

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