Arabidopsis CYP94B3 Encodes Jasmonyl-l-Isoleucine 12-Hydroxylase, a Key Enzyme in the Oxidative Catabolism of Jasmonate

Kitaoka, Naoki; Matsubara, Takeo; Sato, Michio; Takahashi, Kosaku; Wakuta, Shinji; Kawaide, Hiroshi; Matsui, Hirokazu; Nabeta, Kensuke; Matsuura, Hideyuki

doi:10.1093/pcp/pcr110

Cited by 107 publications

(108 citation statements)

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“…Among the proposed mechanisms involved in the attenuation of JA responses are JA-induced removal of JA-Ile (Kitaoka et al, 2011;Koo et al, 2011;Heitz et al, 2012;Koo and Howe, 2012;Woldemariam et al, 2012), JA-induced expression of MYC2-related TFs that negatively regulate JA responses (Nakata et al, 2013), and, as described here, JA-induced synthesis of stable JAZ repressors Chung et al, 2010;Shyu et al, 2012). Negative regulatory feedback by JAZ10.4 and other stable JAZs may provide stability to the JA network by limiting the range over which the concentrations of signaling components, including MYC-related TFs, JAZs, and JA-Ile, fluctuate during the JA response.…”

Section: Negative Feedback Regulation By Jaz104mentioning

confidence: 99%

Negative Feedback Control of Jasmonate Signaling by an Alternative Splice Variant of JAZ10

et al. 2013

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The plant hormone jasmonate (JA) activates gene expression by promoting ubiquitin-dependent degradation of jasmonate ZIM domain (JAZ) transcriptional repressor proteins. A key feature of all JAZ proteins is the highly conserved Jas motif, which mediates both JAZ degradation and JAZ binding to the transcription factor MYC2. Rapid expression of JAZ genes in response to JA is thought to attenuate JA responses, but little is known about the mechanisms by which newly synthesized JAZ proteins exert repression in the presence of the hormone. Here, we show in Arabidopsis (Arabidopsis thaliana) that desensitization to JA is mediated by an alternative splice variant (JAZ10.4) of JAZ10 that lacks the Jas motif. Unbiased protein-protein interaction screens identified three related basic helix-loop-helix transcription factors (MYC2, MYC3, and MYC4) and the corepressor NINJA as JAZ10.4-binding partners. We show that the amino-terminal region of JAZ10.4 contains a cryptic MYC2-binding site that resembles the Jas motif and that the ZIM motif of JAZ10.4 functions as a transferable repressor domain whose activity is associated with the recruitment of NINJA. Functional studies showed that the expression of JAZ10.4 from the native JAZ10 promoter complemented the JA-hypersensitive phenotype of a jaz10 mutant. Moreover, treatment of these complemented lines with JA resulted in the rapid accumulation of JAZ10.4 protein. Our results provide an explanation for how the unique domain architecture of JAZ10.4 links transcription factors to a corepressor complex and suggest how JA-induced transcription and alternative splicing of JAZ10 premessenger RNA creates a regulatory circuit to attenuate JA responses.

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Section: Negative Feedback Regulation By Jaz104mentioning

confidence: 99%

Negative Feedback Control of Jasmonate Signaling by an Alternative Splice Variant of JAZ10

et al. 2013

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“…1D). [43][44][45] Green leaf volatiles are also produced from 13-HPOT by hydroperoxide lyase (HPL), a P450 belonging to the CYP74B subfamily. 46) CYP74A (AOS) and CYP74B (HPL) are atypical P450s requiring neither oxygen nor an electron for the catalytic activity.…”

Section: P450s In Phytohormone Homeostasis (Cat-egory Iii)mentioning

confidence: 99%

Impacts of Diversification of Cytochrome P450 on Plant Metabolism

Mizutani

2012

Biological & Pharmaceutical Bulletin

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Cytochrome P450 monooxygenases (P450s) catalyze a wide variety of monooxygenation reactions in primary and secondary metabolism in plants. The share of P450 genes in each plant genome is estimated to be up to 1%. This implies that the diversification of P450 has made a significant contribution to the ability to acquire the emergence of new metabolic pathways during land plant evolution. The P450 families conserved universally in land plants contribute to their chemical defense mechanisms. Several P450s are involved in the biosynthesis and catabolism of plant hormones. Species-specific P450 families are essential for the biosynthetic pathways of phytochemicals such as terpenoids and alkaloids. Genome wide analysis of the gene clusters including P450 genes will provide a clue to defining the metabolic roles of orphan P450s. Metabolic engineering with plant P450s is an important technology for large-scale production of valuable phytochemicals such as medicines.Key words P450; metabolic engineering; terpenoid; phytohormone P450S IN THE PLANT KINGDOMMore than 5000 sequences have been stored in a plant P450 database.1) The number of P450 genes in Arabidopsis (Arabidopsis thaliana) is 245, and P450 is the third biggest family gene in Arabidopsis.1) The numbers of P450 genes in several other plants are 316 in grapevine (Vitis vinifera), 332 in soybean (Glycine max), 312 in poplar (Populus trichocarpa), 334 in rice (Oryza sativa), and 372 in sorghum (Sorghum bicolor), which are estimated to represent up to 1% of the total gene annotations of each plant species .2,3) Transcriptome analyses are also contributing to the vast increase in identified P450 sequences from various plant species (http://compbio.dfci. harvard.edu/tgi/plant.html). However, the functions of most of these are still unknown. For instance, in Arabidopsis, the number of functionally characterized P450 genes is about 60, which means that more than 70% of its 245 P450 genes still remain to be characterized. 4)Plant P450s have been shown to participate in a variety of biochemical pathways to produce primary and secondary metabolites such as phenylpropanoids, alkaloids, terpenoids, lipids, cyanogenic glycosides, and glucosinolates, as well as plant hormones.4) The diversification of P450 has had a significant biochemical impact on the emergence of new metabolic pathways during the evolutionary process of land plants. DIVERSIFICATION OF P450 GENES IN LAND PLANTSThe driving force of plant P450 diversification is closely linked to the survival strategy of land plants, conferring advantages in the continuous evolutionary competition and mutualism with other organisms. The role of plant P450 can be discussed in relation to the following categorized reactions and metabolisms: category I, essential reactions conserved in the plant kingdom; category II, core metabolisms conserved in all land plants; category III, essential metabolisms which emerged during the evolution of flowering plants (e.g., plant hormone homeostasis); category IV, secondary metabolisms unique t...

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“…One of the mechanisms for limiting the JA response is inactivation of bioactive JA-Ile by the cytochrome P450 CYP94B3 (Kitaoka et al, 2011;Koo et al, 2011;Heitz et al, 2012;Koo and Howe, 2012). cyp94B3 loss-offunction mutants exhibit increased production of JA-Ile and enhanced expression of JA-responsive genes after wounding, indicating a crucial role for JA-Ile catabolism in attenuation of JA responses.…”

Section: Introductionmentioning

confidence: 99%

A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling inArabidopsis

et al. 2013

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Jasmonates (JAs) are plant hormones that regulate the balance between plant growth and responses to biotic and abiotic stresses. Although recent studies have uncovered the mechanisms for JA-induced responses in Arabidopsis thaliana, the mechanisms by which plants attenuate the JA-induced responses remain elusive. Here, we report that a basic helix-loophelix-type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1 (JAM1), acts as a transcriptional repressor and negatively regulates JA signaling. Gain-of-function transgenic plants expressing the chimeric repressor for JAM1 exhibited substantial reduction of JA responses, including JA-induced inhibition of root growth, accumulation of anthocyanin, and male fertility. These plants were also compromised in resistance to attack by the insect herbivore Spodoptera exigua. Conversely, jam1 loss-of-function mutants showed enhanced JA responsiveness, including increased resistance to insect attack. JAM1 and MYC2 competitively bind to the target sequence of MYC2, which likely provides the mechanism for negative regulation of JA signaling and suppression of MYC2 functions by JAM1. These results indicate that JAM1 negatively regulates JA signaling, thereby playing a pivotal role in fine-tuning of JA-mediated stress responses and plant growth.

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Arabidopsis CYP94B3 Encodes Jasmonyl-l-Isoleucine 12-Hydroxylase, a Key Enzyme in the Oxidative Catabolism of Jasmonate

Cited by 107 publications

References 32 publications

Negative Feedback Control of Jasmonate Signaling by an Alternative Splice Variant of JAZ10

Negative Feedback Control of Jasmonate Signaling by an Alternative Splice Variant of JAZ10

Impacts of Diversification of Cytochrome P450 on Plant Metabolism

A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling inArabidopsis

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