Arabidopsis assay for mutagenicity

Gichner, T.; Badayev, S.A.; Demchenko, S. I.; Relichová, Jiřina; Sandhu, Shahbeg S.; Usmanov, P. D.; Usmanova, O. V.; Velemínský, J.

doi:10.1016/0027-5107(94)90117-1

Cited by 22 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although there are no published data available on the chemically induced clastogenic effect for Brachypodium, the mutagenic effect of MH has previously been demonstrated for a wide of range other plant species such as Crepis capillaris [3], Allium cepa [28, 35, 36], Vicia faba [37] and Tradescantia [38]. In contrast, according to Gichner et al [39], MH is not mutagenic in Arabidopsis cells. In barley, MH at a concentration of 3 mM induced the micronuclei at almost a two-fold higher frequency (8.8%) [5] than in Brachypodium (4.8%).…”

Section: Resultsmentioning

confidence: 99%

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

2017

View full text Add to dashboard Cite

Brachypodium distachyon (Brachypodium) is now intensively utilized as a model grass species in various biological studies. Its favorable cytological features create a unique foundation for a convenient system in mutagenesis, thereby potentially enabling the ‘hot spots’ and ‘cold spots’ of DNA damage in its genome to be analyzed. The aim of this study was to analyze the involvement of 5S rDNA, 25S rDNA, the Arabidopsis-type ()n telomeric sequence and the Brachypodium-originated centromeric BAC clone CB33J12 in the micronuclei formation in Brachypodium root tip cells that were subjected to the chemical clastogenic agent maleic hydrazide (MH). To the best of our knowledge, this is the first use of a multicolor fluorescence in situ hybridization (mFISH) with four different DNA probes being used simultaneously to study plant mutagenesis. A quantitative analysis allowed ten types of micronuclei, which were characterized by the presence or absence of specific FISH signal(s), to be distinguished, thus enabling some specific rules governing the composition of the MH-induced micronuclei with the majority of them originating from the terminal regions of chromosomes, to be identified. The application of rDNA sequences as probes showed that 5S rDNA-bearing chromosomes are involved in micronuclei formation more frequently than the 25S rDNA-bearing chromosomes. These findings demonstrate the promising potential of Brachypodium to be a useful model organism to analyze the effects of various genotoxic agents on the plant nuclear genome stability, especially when the complex FISH-based and chromosome-specific approaches such as chromosome barcoding and chromosome painting will be applied in future studies.

show abstract

Section: Resultsmentioning

confidence: 99%

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

2017

View full text Add to dashboard Cite

show abstract

“…The concentrations used and time of exposure to NaN 3 in the present study, as well as the pH and temperature, were determined on the basis of published reports. The pH of the phosphate buffer used in the present study was 3 on the basis of the report of Gichner et al (1994), who determined that the most favorable pH for treatment with NaN 3 is pH 3.…”

Section: Discussionmentioning

confidence: 99%

Effects of the Application of Different Concentrations of NaN3 for Different Times on the Morphological and Cytogenetic Characteristics of Barley (Hordeum vulgare L.) Seedlings

Ilbaş

Eroğlu

2005

J Integrative Plant Biology

View full text Add to dashboard Cite

Sodium azide (NaN 3 ) has been used in many biological studies as a mutagen. In the present study, the morphological and cytogenetic effects of NaN 3 on barley (Hordeum vulgare L.) seedlings were investigated. Seeds of barley were treated with different concentrations of NaN 3 (0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mmol/L) and applied for different periods of time (3 and 4 h). Parameters investigated, except for the mitotic index, were determined on Days 7 and 14. Increasing concentrations of NaN 3 affected germination rates on Days 7 and 14 following application for 3 and 4 h. Although the length of the roots and leaves was affected by treatment with NaN 3 on Day 14 of the germination period, coleoptile length was affected by NaN 3 treatment on Day 7. Increasing concentrations of NaN 3 and increased treatment period decreased the mitotic index compared with the untreated control. The inhibitory effects of NaN 3 on the mitotic index indicate that NaN 3 can have genotoxic and mutagenic effects on barley seedlings.

show abstract

“…In contrast to the situation in other plants, MH is not mutagenic in A. thaliana [28]. In V. faba, root meristems treatment with MH doses in the range of 4-16 mM for 0.5 h at pH 5.4 caused 35-80% of metaphases with aberrations [29].…”

Section: The Uracil Analogue Maleic Hydrazidementioning

confidence: 99%

DNA damage and repair in Arabidopsis thaliana as measured by the comet assay after treatment with different classes of genotoxins

Menke

Chen

Angelis

et al. 2001

Mutation Research/Genetic Toxicology and Environmental Mutagene

142

View full text Add to dashboard Cite

The three protocols of the comet assay A/N, A/A and N/N were for the first time applied to the plant species Arabidopsis thaliana. The purpose of the experiments was to establish conditions for genotoxic exposure causing DNA damage in Arabidopsis nuclei. This is required for comprehensive gene expression profiling with the intention to screen for genes involved in response of Arabidopsis cells to genotoxic stress.Five chemicals belonging to different classes of mutagens (the monofunctional alkylating agents N-methyl-N-nitrosourea and methyl methanesulfonate, the polyfunctional alkylating agent mitomycin C, the radiomimetic bleomycin and the herbicide maleic hydrazide) were tested. Except for maleic hydrazide, dose-dependent increases in DNA damage were found using the A/N comet assay protocol. While a rapid repair of bleomycin-mediated SSBs and DSBs was found, no significant reduction of DNA migration was observed up to 48 h after treatment with the monofunctional alkylating agents.

show abstract

Arabidopsis assay for mutagenicity

Cited by 22 publications

References 5 publications

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

Effects of the Application of Different Concentrations of NaN3 for Different Times on the Morphological and Cytogenetic Characteristics of Barley (Hordeum vulgare L.) Seedlings

DNA damage and repair in Arabidopsis thaliana as measured by the comet assay after treatment with different classes of genotoxins

Contact Info

Product

Resources

About