Aqueous biphasic systems containing PEG-based deep eutectic solvents for high-performance partitioning of RNA

Zhang, Hongmei; Wang, Yuzhi; Zhou, Yigang; Xu, Kaijia; Li, Na; Wen, Qian; Yang, Qin

doi:10.1016/j.talanta.2017.04.018

Cited by 70 publications

(36 citation statements)

References 37 publications

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“…The compatibility of the developed system with mild processing steps makes it promising not only for the encapsulation of cellular cargo, but also to broader applications involving sensitive molecules, including proteins and RNAs. [53,54] To prove our concept, the immiscible ATPS dextran/PEG phases were used to mix oppositely charged polyelectrolytes. Despite the characteristic low interfacial tension of ATPS, [55] the dropwise addition of the dispersed Dex+ALG phase into the continuous PEG+PLL phase, under agitation, led to the formation of single capsules with firm spherical morphology, showing that the interfacial tension and probable immediate initiation of interfacial complexation was enough to ensure that the system was kept stable, avoiding droplet's breakage.…”

Section: Discussionmentioning

confidence: 99%

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One‐Step All‐Aqueous Interfacial Assembly of Robust Membranes for Long‐Term Encapsulation and Culture of Adherent Stem/Stromal Cells

Vilabril

Nadine

Neves

et al. 2021

Adv Healthcare Materials

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The therapeutic effectiveness and biological relevance of technologies based on adherent cells depend on platforms that enable long-term culture in controlled environments. Liquid-core capsules have been suggested as semipermeable moieties with spatial homogeneity due to the high mobility of all components in their core. The lack of cell-adhesive sites in liquid-core structures often hampers their use as platforms for stem cell-based technologies for long-term survival and cell-directed self-organization. Here, the one-step fast formation of robust polymeric capsules formed by interfacial complexation of oppositely charged polyelectrolytes in an all-aqueous environment, compatible with the simultaneous encapsulation of mesenchymal stem/stromal cells (MSCs) and microcarriers, is described. The adhesion of umbilical cord MSCs to polymeric microcarriers enables their aggregation and culture for more than 21 days in capsules prepared either manually by dropwise addition, or by scalable electrohydrodynamic atomization, generating robust and stable capsules. Cell aggregation and secretion overtime can be tailored by providing cells with static or dynamic (bioreactor) environments.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

One‐Step All‐Aqueous Interfacial Assembly of Robust Membranes for Long‐Term Encapsulation and Culture of Adherent Stem/Stromal Cells

Vilabril

Nadine

Neves

et al. 2021

Adv Healthcare Materials

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“…25 Zhang et al investigated the ABSs formed by novel PEG-based DESs and used them for RNA extraction. 26 Xu et al discovered novel ABSs composed of ILs and DESs for DNA extraction. 27 To further enrich the category of ABSs, ABSs composed of two different DESs are studied in this work.…”

Section: Introductionmentioning

confidence: 99%

Development of different deep eutectic solvent aqueous biphasic systems for the separation of proteins

et al. 2019

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“…A DES is obtained by mixing two or more cheap and green components, including the hydrogen bond acceptor (HBA) and the hydrogen bond donor (HBD), with the ability to relate to each other by hydrogen bond interactions. The obtained chemical entity has a melting point lower than that of the initial compounds [23,24]. The mostly DESs in LPME are obtained by mixing choline chloride with phenols (chlorophenol, phenol) [25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Air‐assisted dispersive liquid–liquid microextraction based on a new hydrophobic deep eutectic solvent for the preconcentration of benzophenone‐type UV filters from aqueous samples

Zhang

Dai

et al. 2018

J of Separation Science

102

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Deep eutectic solvents are considered as new and green solvents that can be widely used in analytical chemistry such as microextraction. In the present work, a new dl-menthol-based hydrophobic deep eutectic solvent was synthesized and used as extraction solvents in an air-assisted dispersive liquid-liquid microextraction method for preconcentration and extraction of benzophenone-type UV filters from aqueous samples followed by high-performance liquid chromatography with diode array detection. In an experiment, the deep eutectic solvent formed by dl-menthol and decanoic acid was added to an aqueous solution containing the UV filters, and then the mixture was sucked up and injected five times by using a glass syringe, and a cloudy state was achieved. After extraction, the solution was centrifuged and the upper phase was subjected to high-performance liquid chromatography for analysis. Various parameters such as the type and volume of the deep eutectic solvent, number of pulling, and pushing cycles, solution pH and salt concentration were investigated and optimized. Under the optimum conditions, the developed method exhibited low limits of detection and limits of quantitation, good linearity, and precision. Finally, the proposed method was successfully applied to determine the benzophenone-type filters in environmental water samples with relative recoveries of 88.8-105.9%.

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Aqueous biphasic systems containing PEG-based deep eutectic solvents for high-performance partitioning of RNA

Cited by 70 publications

References 37 publications

One‐Step All‐Aqueous Interfacial Assembly of Robust Membranes for Long‐Term Encapsulation and Culture of Adherent Stem/Stromal Cells

One‐Step All‐Aqueous Interfacial Assembly of Robust Membranes for Long‐Term Encapsulation and Culture of Adherent Stem/Stromal Cells

Development of different deep eutectic solvent aqueous biphasic systems for the separation of proteins

Air‐assisted dispersive liquid–liquid microextraction based on a new hydrophobic deep eutectic solvent for the preconcentration of benzophenone‐type UV filters from aqueous samples

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