Aqueous Ascorbate Concentration in Hereditary Buphthalmic Rabbits

Ascorbate in Aqueous Humor Augments Nitric Oxide Production by Macrophages

“…Therefore, we tested whether the anti-oxidant ascorbate, which is present in very high concentrations within AqH (27), was responsible for interference with the Griess Assay. Ascorbate was measured in four lots of rabbit AqH and the average concentration (1.0 ± 0.6 mm) was comparable to concentrations observed in a previous report (28). For comparison the concentration of ascorbate in rabbit serum was 30 µM (28).…”

“…Ascorbate was measured in four lots of rabbit AqH and the average concentration (1.0 ± 0.6 mm) was comparable to concentrations observed in a previous report (28). For comparison the concentration of ascorbate in rabbit serum was 30 µM (28). As shown in Figure 7B, a 1mM ascorbate solution also completely inhibited measurement of nitrite standards by the Griess assay.…”

Ascorbate in Aqueous Humor Augments Nitric Oxide Production by Macrophages

“…Therefore, we tested whether the anti-oxidant ascorbate, which is present in very high concentrations within AqH (27), was responsible for interference with the Griess Assay. Ascorbate was measured in four lots of rabbit AqH and the average concentration (1.0 ± 0.6 mm) was comparable to concentrations observed in a previous report (28). For comparison the concentration of ascorbate in rabbit serum was 30 µM (28).…”

“…Ascorbate was measured in four lots of rabbit AqH and the average concentration (1.0 ± 0.6 mm) was comparable to concentrations observed in a previous report (28). For comparison the concentration of ascorbate in rabbit serum was 30 µM (28). As shown in Figure 7B, a 1mM ascorbate solution also completely inhibited measurement of nitrite standards by the Griess assay.…”

Glucose Oxidation in the Buphthalmic Rabbit Cornea

“…In this study peak IOP was recorded at 1-2 months, and at this time the buphthalmic corneas were still clear. These findings are in agreement with those reported by Van Horn et al [1977], Presumably a defect in the aqueous outflow facility is responsible for the initial increase in IOP seen in these rabbits [Hanna et al, 1962], The resulting high IOP leads to decreased aqueous humor formation [MeMaster and Maori, 1967], perhaps by compromising active transport in the ciliary body [Lam et al, 1976]. Such changes in aqueous humor dynamics would account for the fall in IOP seen in advanced buphthalmia.…”

Intraocular Pressure Responses to Antiglaucoma Agents in Spontaneous Buphthalmic Rabbits

“…The gene for transmission of the disease is said to be semilethal [Hanna et al, 1962], Furthermore, as observed in the present study, as well as by others, buph thalmic rabbits breed poorly, have a low incidence of ocular hypertension (20% in the present study) and exhibit a period of IOP elevation which is limited to only a few months [MacMaster and Macri, 1967;Lam, 1976;Van Horn et al, 1977;Gelatt, 1977]. Considerable difficulties are thus encountered in maintaining a sufficiently large colony for routine drug evaluation.…”

Ascorbate in Aqueous Humor Augments Nitric Oxide Production by Macrophages

“…Therefore, we tested whether the anti-oxidant ascorbate, which is present in very high concentrations within AqH (27), was responsible for interference with the Griess Assay. Ascorbate was measured in four lots of rabbit AqH and the average concentration (1.0 ± 0.6 mm) was comparable to concentrations observed in a previous report (28). For comparison the concentration of ascorbate in rabbit serum was 30 µM (28).…”

“…Ascorbate was measured in four lots of rabbit AqH and the average concentration (1.0 ± 0.6 mm) was comparable to concentrations observed in a previous report (28). For comparison the concentration of ascorbate in rabbit serum was 30 µM (28). As shown in Figure 7B, a 1mM ascorbate solution also completely inhibited measurement of nitrite standards by the Griess assay.…”

Glucose Oxidation in the Buphthalmic Rabbit Cornea

“…In this study peak IOP was recorded at 1-2 months, and at this time the buphthalmic corneas were still clear. These findings are in agreement with those reported by Van Horn et al [1977], Presumably a defect in the aqueous outflow facility is responsible for the initial increase in IOP seen in these rabbits [Hanna et al, 1962], The resulting high IOP leads to decreased aqueous humor formation [MeMaster and Maori, 1967], perhaps by compromising active transport in the ciliary body [Lam et al, 1976]. Such changes in aqueous humor dynamics would account for the fall in IOP seen in advanced buphthalmia.…”

Intraocular Pressure Responses to Antiglaucoma Agents in Spontaneous Buphthalmic Rabbits

“…The gene for transmission of the disease is said to be semilethal [Hanna et al, 1962], Furthermore, as observed in the present study, as well as by others, buph thalmic rabbits breed poorly, have a low incidence of ocular hypertension (20% in the present study) and exhibit a period of IOP elevation which is limited to only a few months [MacMaster and Macri, 1967;Lam, 1976;Van Horn et al, 1977;Gelatt, 1977]. Considerable difficulties are thus encountered in maintaining a sufficiently large colony for routine drug evaluation.…”

Ascorbate in Aqueous Humor Augments Nitric Oxide Production by Macrophages

“…Therefore, we tested whether the anti-oxidant ascorbate, which is present in very high concentrations within AqH (27), was responsible for interference with the Griess Assay. Ascorbate was measured in four lots of rabbit AqH and the average concentration (1.0 ± 0.6 mm) was comparable to concentrations observed in a previous report (28). For comparison the concentration of ascorbate in rabbit serum was 30 µM (28).…”

“…Ascorbate was measured in four lots of rabbit AqH and the average concentration (1.0 ± 0.6 mm) was comparable to concentrations observed in a previous report (28). For comparison the concentration of ascorbate in rabbit serum was 30 µM (28). As shown in Figure 7B, a 1mM ascorbate solution also completely inhibited measurement of nitrite standards by the Griess assay.…”

Glucose Oxidation in the Buphthalmic Rabbit Cornea

“…In this study peak IOP was recorded at 1-2 months, and at this time the buphthalmic corneas were still clear. These findings are in agreement with those reported by Van Horn et al [1977], Presumably a defect in the aqueous outflow facility is responsible for the initial increase in IOP seen in these rabbits [Hanna et al, 1962], The resulting high IOP leads to decreased aqueous humor formation [MeMaster and Maori, 1967], perhaps by compromising active transport in the ciliary body [Lam et al, 1976]. Such changes in aqueous humor dynamics would account for the fall in IOP seen in advanced buphthalmia.…”

Intraocular Pressure Responses to Antiglaucoma Agents in Spontaneous Buphthalmic Rabbits

“…The gene for transmission of the disease is said to be semilethal [Hanna et al, 1962], Furthermore, as observed in the present study, as well as by others, buph thalmic rabbits breed poorly, have a low incidence of ocular hypertension (20% in the present study) and exhibit a period of IOP elevation which is limited to only a few months [MacMaster and Macri, 1967;Lam, 1976;Van Horn et al, 1977;Gelatt, 1977]. Considerable difficulties are thus encountered in maintaining a sufficiently large colony for routine drug evaluation.…”