Aquasorbent guargum grafted hyperbranched poly (acrylic acid): A potential culture medium for microbes and plant tissues

Das, T. K.; Sengupta, Samarjit; Pal, Abhijit; Sardar, Soumen; Sahu, Nilanjan; Lenka, Naisargik; Panigrahi, Kishore C. S.; Goswami, Luna; Bandyopadhyay, Abhijit

doi:10.1016/j.carbpol.2019.114983

Cited by 7 publications

(4 citation statements)

References 42 publications

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“…Designing a matrix for supporting or hardening plant tissue culture medium using a BC-derived gelling agent under the acronym PLATIBACGEL has been challenging for the research team. It was expensive and overexploited when agar was predominantly used as the gelling agent for microbial and plant cell culture media (Das et al 2019). Others have voiced concerns regarding agar applicability, especially for its limited resources overuse, and considerations include exorbitant pricing (Hassan and Moubarak 2020;Hegele et al 2021).…”

Section: Mechanical Strengthmentioning

confidence: 99%

“…Furthermore, the natural hydrocolloid extracted from the seeds of the carob tree (Ceratonia siliqua L.), namely "LBG," was analyzed for its potential as plant tissue culture media (Gonçalves and Romano 2005). Recently, guar gum has also been explored in this context (Das et al 2019). Studies were also extended to investigate a wide range of plant species and crops in an attempt to screen the best ingredients to be used in explants and medium components.…”

Section: Mechanical Strengthmentioning

confidence: 99%

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Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

et al. 2022

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Bacterial cellulose (BC) is a natural biodegradable, eco-friendly fiber, lying within the nanoscale range. It is reputable for its various physical and chemical qualities, like high hydrophilicity, immense crystallinity, ease of sterility, being toxin-free, and extremely pure. Adding to its wide applicability in different fields, this study evaluated the applicability of a developed gelling substitute for plant tissue culture media. The BC matrix was characterized under the acronym PLATIBACGEL (PLAnt TIssue Culture BActerial Cellulose GEL), formed by Komagataeibacter hansenii AS.5, preisolated from rotten apple waste. Scanning electron microscope, Fourier-transform infrared, X-ray diffractometer, and tensile strength analyses confirmed the formation of purified, porous, and heterogeneous densely packed multiple network polymers possessing cellulose properties. The water holding capacity (WHC) values of wet and dried BC membranes were 9179% and 226.9%, respectively, and the water absorption rate (WAR) of dry BC membranes was higher than that of wet membranes. Using BC as a tissue culture gelling agent, six genotypes from tomato and wheat seeds were cultured in vitro, for guaranteeing explant genetic diversity, over seven treatments. Treatment 5, included PLATIBACGEL as the main constituent, improved and sustained all in vitro seed germination, root penetration, and plant support. Likewise, repeated tomato micropropagation subcultures were successful. Results demonstrated applying PLATIBACGEL as a promising, reusable, cheap, and reliable alternative plant micropropagation media gelling agent. Wherefore, plant cellular developers and tissue-culturists can utilize bio-polymers like BC for better understanding plant cell response to different in vitro culturing conditions, with expected beneficial returns on gelling agents industry and markets as well. Graphical abstract

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Section: Mechanical Strengthmentioning

confidence: 99%

Section: Mechanical Strengthmentioning

confidence: 99%

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

et al. 2022

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“…Conventionally, agar, a natural polysaccharide, is used to solidify in vitro plant culture media due to its gelling properties, stability, resistance to plant metabolic enzymes and high clarity [28]. Regardless of its natural origins, agar is typically the most expensive constituent of plant tissue culture media and different gums and starches have been studied throughout the years as gelling agent alternatives [28][29][30][31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

“…The rheological and microstructural features of semi-solid and gel-like plant culture media are expected to influence plant growth and development. However, literature is surprisingly scarce on this topic [28,30]. While rheology provides information about the viscoelastic flow behaviour and structure of materials [40], the microstructural analysis provides insights into the morphology of the system, such as roughness, pore size, surface area and other textural features [41,42].…”

Section: Introductionmentioning

confidence: 99%

Rheological and Microstructural Features of Plant Culture Media Doped with Biopolymers: Influence on the Growth and Physiological Responses of In Vitro-Grown Shoots of Thymus lotocephalus

et al. 2021

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In vitro culture is an important biotechnological tool in plant research and an appropriate culture media is a key for a successful plant development under in vitro conditions. The use of natural compounds to improve culture media has been growing and biopolymers are interesting alternatives to synthetic compounds due to their low toxicity, biodegradability, renewability, and availability. In the present study, different culture media containing one biopolymer (chitosan, gum arabic) or a biopolymer derivative [hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC)], at 100 or 1000 mg L−1, were tested regarding their influence on the growth and physiological responses of Thymus lotocephalus in vitro culture. Cellulose-based biopolymers (HEC and CMC) and gum arabic were used for the first time in plant culture media. The results showed that CMC at 100 mg L−1 significantly improved shoot elongation while chitosan, at the highest concentration, was detrimental to T. lotocephalus. Concerning only the evaluated physiological parameters, all tested biopolymers and biopolymer derivatives are safe to plants as there was no evidence of stress-induced changes on T. lotocephalus. The rheological and microstructural features of the culture media were assessed to understand how the biopolymers and biopolymer derivatives added to the culture medium could influence shoot growth. As expected, all media presented a gel-like behaviour with minor differences in the complex viscosity at the beginning of the culture period. Most media showed increased viscosity overtime. The surface area increased with the addition of biopolymers and biopolymer derivatives to the culture media and the average pore size was considerably lower for CMC at 100 mg L−1. The smaller pores of this medium might be related to a more efficient nutrients and water uptake by T. lotocephalus shoots, leading to a significant improvement in shoot elongation. In short, this study demonstrated that the different types of biopolymers and biopolymer derivatives added to culture medium can modify their microstructure and at the right concentrations, are harmless to T. lotocephalus shoots growing in vitro, and that CMC improves shoot length.

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Advances in chitosan-based drug delivery systems: A comprehensive review for therapeutic applications

Haider,

Khan,

Iqbal

et al. 2024

European Polymer Journal

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Aquasorbent guargum grafted hyperbranched poly (acrylic acid): A potential culture medium for microbes and plant tissues

Cited by 7 publications

References 42 publications

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

Rheological and Microstructural Features of Plant Culture Media Doped with Biopolymers: Influence on the Growth and Physiological Responses of In Vitro-Grown Shoots of Thymus lotocephalus

Advances in chitosan-based drug delivery systems: A comprehensive review for therapeutic applications

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