Aptamers: current challenges and future prospects

Rozenblum, Guido Tomás; Lopez, Vanina Gisela; Vitullo, Alfredo Daniel; Radrizzani, Martı́n

doi:10.1517/17460441.2016.1126244

Cited by 75 publications

(48 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This process is repeated as many times as necessary (usually 8-15 rounds) until aptamers with the desired properties are selected. While the described process seems to be easy to apply, this is far from simple [5] and it gets more complex when aptamers must be selected as a probe for an electrochemical sensor. In many electrochemical sensors, aptamers might need to be modified with at least an anchoring chemical group for surface immobilization and a redox-active dye to generate a measurable signal.…”

Section: Aptamer Selection For Electrochemical Biosensing Devicesmentioning

confidence: 99%

“…Although therapeutic aptamer developments are facing skepticism [5], it is in the field of biosensors that aptamers could maximize their potential, and original research reports are showing how aptamers can be used to provide novel diagnostic solutions. However, in our opinion, there are still several limitations that aptamer electrochemical-based biosensors need to overcome, including the development of a systematic aptamer discovery process for biosensors, chemisorption of molecules on the conductive surface, quality of the semiconductor material, and improvement of the signal-to-noise ratio.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Challenges in Electrochemical Aptasensors and Current Sensing Architectures Using Flat Gold Surfaces

Rozenblum

Pollitzer²,

Radrizzani

2019

Chemosensors

View full text Add to dashboard Cite

In recent years, reagentless aptamer biosensors, named aptasensors, have shown significant advancements. Particularly, electrochemical aptasensors could change the field of biosensors in this era, where digitalization seems to be a common goal of many fields. Biomedical devices are integrating electronic technologies for detecting pathogens, biomolecules, small molecules, and ions, and the physical-chemical properties of nucleic acid aptamers makes them very interesting for these devices. Aptamers can be easily synthesized and functionalized with functional groups for immobilization and with redox chemical groups that allow for the conversion of molecular interactions into electrical signals. Furthermore, non-labeled aptamers have also been utilized. This review presents the current challenges involved in aptasensor architectures based on gold electrodes as transducers.

show abstract

Section: Aptamer Selection For Electrochemical Biosensing Devicesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Challenges in Electrochemical Aptasensors and Current Sensing Architectures Using Flat Gold Surfaces

Rozenblum

Pollitzer²,

Radrizzani

2019

Chemosensors

View full text Add to dashboard Cite

show abstract

“…These are short sequences of nucleic acids which have a secondary structure with a structural affinity towards a given analyte or which acquire a three-dimensional structure upon analyte binding [66]. These interactions are ruled by a combination of pi bond stacking, London dispersion forces and hydrogen bonding [67,68]. Some aptamers have been developed to bind to wide range of analytes such as metal ions [69]], proteins [70,71] and whole cells [72].…”

Section: General Principles Of Aunp-based Biomolecular Recognitionmentioning

confidence: 99%

Gold Nanoparticles for Diagnostics: Advances towards Points of Care

et al. 2016

View full text Add to dashboard Cite

The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted developments in the exploration of biomolecular interactions with AuNP-containing systems, in particular for biomedical applications in diagnostics. These systems show great promise in improving sensitivity, ease of operation and portability. Despite this endeavor, most platforms have yet to reach maturity and make their way into clinics or points of care (POC). Here, we present an overview of emerging and available molecular diagnostics using AuNPs for biomedical sensing that are currently being translated to the clinical setting.

show abstract

“…This is a clear advantage over antibodies which are produced under strict physiological conditions 28 . In addition, aptamers can be easily modified and therefore be optimized for various sensing platforms such as lateral flow assays, surface plasmon resonance sensors, flow cytometry or fluorescence microscopy 29 .…”

Section: Introductionmentioning

confidence: 99%

“…Cell-SELEX can be used to select aptamers binding to whole living cells and, thus, eliminates the need for prior knowledge of a target molecule 32 . Rounds of counter-selection are typically used to reduce aptamer cross-reactivity across targets by eliminating non-specific aptamers 29 . Cell-SELEX has been successfully employed to isolate aptamers against various bacterial species such as E. coli, Salmonella typhimurium, Campylobacter jejuni, Listeria monocytogenes, Staphylococcus aureus and Vibrio parahaemolyticus 28, 33–37 .…”

Section: Introductionmentioning

confidence: 99%

Identification of two aptamers binding toLegionella pneumophilawith high affinity and specificity

Saad

Chinerman

Tabrizian

et al. 2019

Preprint

View full text Add to dashboard Cite

19Legionella pneumophila (Lp) is a water borne bacterium causing Legionnaires' Disease (LD) in 20 humans. Rapid detection of Lp in water system is essential to reduce the risk of LD outbreaks. 21 The methods currently available require expert skills and are time intensive, thus delaying 22 intervention. In situ detection of Lp by biosensor would allow rapid implementation of control 23 strategies. To this end, a biorecognition element is required. Aptamers are considered promising 24 biorecognition molecules for biosensing. Aptamers are short oligonucleotide sequence folding 25 into a specific structure and are able to bind to specific molecules. Currently no aptamer and thus 26 no aptamer-based technology exists for the detection of Lp. In this study, Systemic Evolution of 27 Ligands through EXponential enrichment (SELEX) was used to identify aptamers binding 28 specifically to Lp. Ten rounds of positive selection and two rounds of counter-selection against 29 two Pseudomonas species were performed. Two aptamers binding strongly to Lp were identified 30 with KD of 116 and 135 nM. Binding specificity of these two aptamers to Lp was confirmed by 31 flow cytometry and fluorescence microscopy. Therefore, these two aptamers are promising 32 biorecognition molecules for the detection of Lp in water systems. 33 34 Key words: Legionella pneumophila, aptamers, SELEX, flow cytometry, fluorescence 35 microscopy, Pseudomonas 36 37 38 Legionella pneumophila (Lp) is a pathogenic Gram-negative bacterium responsible for two types 39 of respiratory diseases, namely the severe pneumonia Legionnaires' Disease (LD) and the milder 40 flu-like Pontiac fever 1 . Lp occurs in both natural and engineered water systems and is one of the 41 most prevalent pathogens in man-made, engineered water systems 2 . Infections occur when the 42 bacteria are aerosolized, and the contaminated aerosols are inhaled; Lp can then infect and replicate 43 inside alveolar macrophages 3 . Modern water systems provide optimal transmission conditions for 44 Lp by generating aerosols 4 . Leading sources of infection are cooling towers, hot water distribution 45 systems, humidifiers, misters, showers, fountains, spa pools and evaporative condensers 5 . 46 47 Outbreaks of LD consistently occur globally and have increased in recent years. The average 48 incidence rate is about 10-15 cases per million people 6 . According to the Centre for Disease 49 Control, incidences of legionellosis have increased by four and a half times between 2000 and 50 2016 7 . The rise in LD outbreaks can be attributed to several factors such as aging infrastructures 51and an aging population who is more vulnerable to such infections, as well as increases in diagnosis 52 and reporting 8,9 . Nevertheless, most LD outbreaks are the consequences of management failure of 53 man-made water systems 10 . Examples of these failures for water distribution systems include 54 keeping the temperature of hot water distribution system below 50°C and allowing water to 55 stagnate 10 . For coolin...

show abstract

Aptamers: current challenges and future prospects

Cited by 75 publications

References 78 publications

Challenges in Electrochemical Aptasensors and Current Sensing Architectures Using Flat Gold Surfaces

Challenges in Electrochemical Aptasensors and Current Sensing Architectures Using Flat Gold Surfaces

Gold Nanoparticles for Diagnostics: Advances towards Points of Care

Identification of two aptamers binding toLegionella pneumophilawith high affinity and specificity

Contact Info

Product

Resources

About