Aptamer–Exosomes for Tumor Theranostics

Yi, Kezhen; Yuan, Rong; Huang, Lanxiang; Tang, Xuan; Zhang, Qian; Wang, Wei; Wu, Jiangquan; Wang, Xinghuan

doi:10.1021/acssensors.0c02237

Cited by 31 publications

(26 citation statements)

References 100 publications

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“…(C) EMBNs formed by EVs are used for drug delivery during tumor therapy. [ 46 ] Reproduced with permission from Ref. [46].…”

Section: The Species Of Embnsmentioning

confidence: 99%

“…[ 45 ] Researchers have developed EVs from tumor cells for the targeted delivery of anticancer drugs to treat cancer, with promising results (Figure 3C). [ 46 ] More importantly, clinically, most patients with the novel coronavirus infection COVID‐19 have mild symptoms, approximately 20% of these patients will develop severe diseases such as systemic immune disorders caused by a cytokine storm. Thus, for novel coronavirus infection, it is essential to regulate cytokine levels throughout the body.…”

Section: The Species Of Embnsmentioning

confidence: 99%

See 1 more Smart Citation

Construction of Nanocarriers Based on Endogenous Cell Membrane and Their Application in Nanomedicine

Guo

Liu

et al. 2022

Chin. J. Chem.

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Comprehensive Summary Inspired by the adaptation of cells to the surrounding environment, the combination of cell membrane and nanomaterials has gradually become a new type of biomimetic nanocomposite. The construction of endogenous membrane biomimetic nanocarriers (EMBNs) not only retains the structure of membrane surface protein, but also has the properties of nanoparticles (NPs), also provides the ability of natural interaction between nanomaterials and organisms, thus overcoming the severe challenges faced by traditional nanodelivery systems in clinical application. In this paper, the construction methods of EMBNs are reviewed, focusing on a simple method to prepare membrane proteins. Secondly, EMBNs were classified according a variety of cell membranes. Finally, the unique advantages of EMBNs in nanomedicine are introduced, including improving biocompatibility, homologous targeting ability, prolonging blood circulation time and immune escape ability. In nanomedicine, such as targeted delivery, phototherapy, immunotherapy and tumor imaging, multifaceted biological interfaces through membrane masking provide a new approach for the development of multifunctional NPs.

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“…(C) EMBNs formed by EVs are used for drug delivery during tumor therapy. [ 46 ] Reproduced with permission from Ref. [46].…”

Section: The Species Of Embnsmentioning

confidence: 99%

Section: The Species Of Embnsmentioning

confidence: 99%

Construction of Nanocarriers Based on Endogenous Cell Membrane and Their Application in Nanomedicine

Guo

Liu

et al. 2022

Chin. J. Chem.

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“…In particular, exosomes derived from cancer cells are involved in mediating intercellular communication to facilitate cancer growth and metastasis [3][4][5] . It has been reported that the expression of exosomal membrane proteins such as CD8, CD63, EpCAM, PCAT-1 and PSA is much higher on cancerous exosomes than on noncancerous exosomes 5,6 . Therefore, exosomes have been proposed as biomarkers for the early noninvasive diagnosis of cancer 7,8 .…”

Section: Introductionmentioning

confidence: 99%

Fast and specific enrichment of cancer-related exosomes by DNA-nanoweight-assisted centrifugation

yuxyan

Shi³

et al. 2021

Preprint

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Exosomes are nanoscale membrane vesicles actively released by cells and play an important role in the diagnosis of cancer-related diseases. However, it is challenging to efficiently enrich exosomes from extracellular fluids. In this work, we used DNA-tetrahedron as a nanoweight during centrifugation to precisely enrich tumor exosomes from a complex biological environment. Two different DNA tetrahedral nanostructures (DTAs), each carrying a specific aptamer for exosome biomarker recognition, were incubated with clinical samples simultaneously. One DTA triggered the cross-linking of multiple target exosomes, and therefore enabled low-speed and fast centrifugation for enrichment. The other DTA further narrowed down the target exosome subtype and initiated a hybridization chain reaction (HCR) for sensitive signal amplification. The method enabled the detection of 180 MCF-7-derived exosomes per microliter and 560 HepG2-derived exosomes per microliter, with 1000-fold higher sensitivity than conventional ELISA. This easy-to-operate method can enrich exosomes with excellent specificity and therefore will be appealing in biomedical research and clinical diagnosis.

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“…10 As a complementary method of antibody-based methods, nucleic acid tests use stable and easy-to-synthesis DNA probes. 11,12 Nucleic acid-based techniques for S. enterica detection include isothermal amplification and polymerase chain reaction (PCR) strategies. PCR-based approaches are sensitive for detecting foodborne pathogens, but the amplification procedures require precise temperature control.…”

mentioning

confidence: 99%

“…Nevertheless, antibodies are costly and hard to preserve . As a complementary method of antibody-based methods, nucleic acid tests use stable and easy-to-synthesis DNA probes. , Nucleic acid-based techniques for S. enterica detection include isothermal amplification and polymerase chain reaction (PCR) strategies.…”

mentioning

confidence: 99%

G-Quadruplex-Probing CRISPR-Cas12 Assay for Label-Free Analysis of Foodborne Pathogens and Their Colonization In Vivo

Xia

Zhang

et al. 2021

ACS Sens.

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Foodborne pathogen infection is a key issue of food safety. Herein, we developed a label-free assay for Salmonella enterica (S. enterica) detection based on the G-quadruplex-probing CRISPR-Cas12 system (termed G-CRISPR-Cas), allowing highly sensitive detection of S. enterica and investigation of their colonization in chickens. The introduction of the G-quadruplex probe serving as the substrate of Cas 12a realized a label-free analysis for foodborne pathogens. Due to the amplification process induced by loop-mediated isothermal amplification (LAMP), G-CRISPR-Cas assay can detect S. enterica as low as 20 CFU. Specificity for pathogenic gene detection was guaranteed by the dual recognition process via LAMP primers and Cas 12a-guided RNA binding. The G-CRISPR-Cas assay was applied to explore S. enterica colonization in the intestinal tract and organs of chickens and showed the risk of S. enterica infection outside of the intestinal tract. The G-CRISPR-Cas assay is promising for on-site diagnosis of the infection or contamination of foodborne pathogens outside the laboratories, such as abattoirs and markets.

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Aptamer–Exosomes for Tumor Theranostics

Cited by 31 publications

References 100 publications

Construction of Nanocarriers Based on Endogenous Cell Membrane and Their Application in Nanomedicine

Construction of Nanocarriers Based on Endogenous Cell Membrane and Their Application in Nanomedicine

Fast and specific enrichment of cancer-related exosomes by DNA-nanoweight-assisted centrifugation

G-Quadruplex-Probing CRISPR-Cas12 Assay for Label-Free Analysis of Foodborne Pathogens and Their Colonization In Vivo

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