Aptabodies – New Type of Artificial Receptors for Detection Proteins

Hianik, Tibor; Porfireva, Anna; Grman, Igor; Evtugyn, Gennady

doi:10.2174/092986608785203656

Cited by 33 publications

(39 citation statements)

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“…Recently, we proposed a simple method for aptamer engineering based on DNA hybridization of the supporting part of the DNA aptamers. This results in the formation of an aptamer dimer, so called here an aptabody, analogous to antibodies, which also possesses two binding sites [5].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…In comparison to canonical aptamers with one binding site, the aptamer dimers display better detection limit and binding constant to human thrombin [5]. It has been also shown that engineered aptamers like multivalent circular constructs exhibit increased stability against cleavage by exonucleases and improved (2 to 3-fold) anticoagulant activity in comparison with canonical single stranded aptamers [8].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…Nowadays, increased interest in aptamer research is focused on improvement of these features for application in medical therapy, biotechnology and biosensor development [3,4]. A novel approach in aptamer engineering is based on dimerization [5][6][7] or multimerization [8], which possess two or more binding sites for the target ligand. Among DNA aptamers, those sensitive to thrombin are most studied.…”

Section: Introductionmentioning

confidence: 99%

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The circular dichroism and differential scanning calorimetry study of the properties of DNA aptamer dimers

Poniková

Tlučková

Antalı́k

et al. 2011

Biophysical Chemistry

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We have applied circular dichroism (CD), temperature-gradient gel electrophoresis (TGGE) and differential scanning calorimetry (DSC) to study the properties of novel bioengineered DNA aptamer dimers sensitive to fibrinogen (F) and heparin (H) binding sites of thrombin and compared them with canonical single stranded aptamer sensitive to fibrinogen binding site of thrombin (Fibri). The homodimer (FF) and heterodimer (FH) aptamers were constructed based on hybridization of their supported parts. CD results showed that both FF and FH dimers form stable guanine quadruplexes in the presence of potassium ions like those in Fibri. The thermal stability of aptamer dimers was slightly lower compared to those of canonical aptamers, but sufficient for practical applications. Both FF and FH aptamer dimers exhibited a potassium-dependent inhibitory effect on thrombin-* Corresponding author: Tel: +421-2-60295683; fax: +421-2-65412305; e-mail: tibor.hianik@fmph.uniba.sk A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 mediated fibrin gel formation, which was on average two-fold higher than those of canonical single stranded Fibri aptamers.

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Section: Accepted M Manuscriptmentioning

confidence: 99%

Section: Accepted M Manuscriptmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The circular dichroism and differential scanning calorimetry study of the properties of DNA aptamer dimers

Poniková

Tlučková

Antalı́k

et al. 2011

Biophysical Chemistry

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“…This supporting part has resulted in a wider structural diversity of aptamers through the formation of aptamer dimers, as was discussed simultaneously by Hasegawa et al (2008) and Hianik et al (2008). Hasegawa et al (2008) described two aptamers sensitive to fibrinogen and heparin binding sites of thrombin which contained poly dT linkers connected by DNA ligase.…”

Section: Structural Polymorphism Of Guanine Quadruplexes and Their Phmentioning

confidence: 92%

“…Fluctuations in the oscillation frequency of the quartz crystal which reflect both mass and viscosity changes at the crystal surface were measured during cycling of the voltage in the range of -0.7 to + 0.6 V (vs. Ag/AgCl reference electrode) a process which aided the interaction between thrombin and the DNA aptamers. This method has substantially improved the thrombin detection limit; while conventional QCM methods with aptamers immobilized by avidin-biotin technology resulted in thrombin detection with a LOD of 10 nM (Hianik et al 2005), the immobilization of aptamers on the MWCNTs-MB composite layer and simultaneous cycling of the voltage resulted in a LOD of 0.3 nM (Evtugyn et al 2008b;Hianik et al 2008). In our work we showed higher sensitivity of aptasensor based on aptamer dimers (aptabodies) in comparison to the conventional single-stranded aptamers (Fig.…”

Section: A C Bmentioning

confidence: 99%

Potential uses of G-quadruplex-forming aptamers

Víglaský

Hianik

2013

gpb

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Abstract. Guanine quadruplex (G-quadruplex) structures are one of a number of structures which are capable of adopting aptamers. G-rich DNA or RNA has an increased propensity to form quadruplex structures which have unusual biophysical and biological properties. G-rich aptamers which form G-quadruplexes have several advantages over unstructured sequences: G-quadruplexes are non-immunogenic, thermodynamically and chemically stable and they have both higher resistance to various serum nucleases and an enhanced cellular uptake. These advantages have led to a number of synthetic oligonucleotides being studied for their potential use as therapeutic agents for cancer therapy and in the treatment of various other diseases. In addition to their suitability in the fields of medicine and biotechnology, these, highly specified, aptameric G-quadruplexes also have great potential in the further development of nano-devices; e.g. basic components in microarrays, microfluidics, sandwich assays and electrochemical biosensors. This review summarizes the biophysical properties of G-quadruplexes and highlights the importance of the stability and recognition properties of aptamers. Examples of the application of aptamers in medical therapy and in biosensors are also discussed.

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Biosensors, Aptamers (Aptasensors)

Bini

Mascini

Turner

2010

Encyclopedia of Industrial Biotechnology

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Aptamers are single‐stranded DNA or RNA ligands which can be selected for different targets, starting from a huge library of molecules containing randomly created sequences. Aptamers have been selected to bind very different targets, from proteins to small organic dyes. The different fields of analytical chemistry, when dealing with systems based on biomolecular interactions, are still dominated by immunoassays, but detailed analytical studies are now demonstrating that some of the limitations of these conventional assays can be circumvented by alternative recognition reagents such as aptamers. Aptamers have been extensively used in many analytical applications such as chromatography, electrophoresis, mass spectrometry, molecular beacons, sensors and biosensors. This chapter focuses on the particular case of biosensors employing aptamers as new biorecognition elements, which are known as “aptasensors”. This chapter describes the specific features of aptamers and reports on their employment for the development of aptasensors, especially as applied to the detection of clinically‐relevant proteins for diagnostic assays. However, the large variety of aptamers which have been selected to bind low molecular weight molecules leads to the possible use of these oligonucleotides in a wider range of applications such as assays for the analysis of environmental and food samples, as well as detection terrorist threat agents that are biological.

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Aptabodies – New Type of Artificial Receptors for Detection Proteins

Cited by 33 publications

References 0 publications

The circular dichroism and differential scanning calorimetry study of the properties of DNA aptamer dimers

The circular dichroism and differential scanning calorimetry study of the properties of DNA aptamer dimers

Potential uses of G-quadruplex-forming aptamers

Biosensors, Aptamers (Aptasensors)

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