Apresentação do primeiro caso autóctone de doença de Chagas diagnosticado no Estado de Santa Catarina, Brasil

Oliveira, Osvaldo Vitorino; Oliveira, Fernando Osvaldo; Neto, J. Abramides

doi:10.1590/s0034-89101970000200007

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2012

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Cited by 5 publications

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“…However, biologically cloned samples were not used and the possibility of a mixed infection was excluded on the basis of only four microsatellite loci. Sylvio X10/1 (a biological clone produced by micromanipulation) was first isolated from a Brazilian patient in 1979 [55] and has been in intermittent sub-culture ever since. The retention of minor maxicircle classes in Sylvio X10/1 for over thirty years suggests that a heteroplasmic state in T. cruzi is naturally sustained.…”

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confidence: 99%

Multiple Mitochondrial Introgression Events and Heteroplasmy in Trypanosoma cruzi Revealed by Maxicircle MLST and Next Generation Sequencing

et al. 2012

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Background Mitochondrial DNA is a valuable taxonomic marker due to its relatively fast rate of evolution. In Trypanosoma cruzi , the causative agent of Chagas disease, the mitochondrial genome has a unique structural organization consisting of 20–50 maxicircles (∼20 kb) and thousands of minicircles (0.5–10 kb). T. cruzi is an early diverging protist displaying remarkable genetic heterogeneity and is recognized as a complex of six discrete typing units (DTUs). The majority of infected humans are asymptomatic for life while 30–35% develop potentially fatal cardiac and/or digestive syndromes. However, the relationship between specific clinical outcomes and T. cruzi genotype remains elusive. The availability of whole genome sequences has driven advances in high resolution genotyping techniques and re-invigorated interest in exploring the diversity present within the various DTUs. Methodology/Principal Findings To describe intra-DTU diversity, we developed a highly resolutive maxicircle multilocus sequence typing (mtMLST) scheme based on ten gene fragments. A panel of 32 TcI isolates was genotyped using the mtMLST scheme, GPI , mini-exon and 25 microsatellite loci. Comparison of nuclear and mitochondrial data revealed clearly incongruent phylogenetic histories among different geographical populations as well as major DTUs. In parallel, we exploited read depth data, generated by Illumina sequencing of the maxicircle genome from the TcI reference strain Sylvio X10/1, to provide the first evidence of mitochondrial heteroplasmy (heterogeneous mitochondrial genomes in an individual cell) in T. cruzi . Conclusions/Significance mtMLST provides a powerful approach to genotyping at the sub-DTU level. This strategy will facilitate attempts to resolve phenotypic variation in T. cruzi and to address epidemiologically important hypotheses in conjunction with intensive spatio-temporal sampling. The observations of both general and specific incidences of nuclear-mitochondrial phylogenetic incongruence indicate that genetic recombination is geographically widespread and continues to influence the natural population structure of TcI, a conclusion which challenges the traditional paradigm of clonality in T. cruzi .

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Apresentação do primeiro caso autóctone de doença de Chagas diagnosticado no Estado de Santa Catarina, Brasil

Abstract: Apresentação do primeiro caso de doença de Chagas, diagnosticado no município de Gaspar, Estado de Santa Catarina, Brasil. Como não se conseguiu demonstrar a presença de triatomíneos na região, não foi possível determinar especìficamente o mecanismo de transmissão.

Cited by 5 publications

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Multiple Mitochondrial Introgression Events and Heteroplasmy in Trypanosoma cruzi Revealed by Maxicircle MLST and Next Generation Sequencing

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