Approach for differentiating trophoblast cell lineage from human induced pluripotent stem cells with retinoic acid in the absence of bone morphogenetic protein 4

Ikeda, Kenji; Kawasaki, Yuki; Matsuda, Hitomi; Kishida, Saho; Iwasaki, Arina; Ohata, Sayaka; Urashima, Yoko; Hirotani, Yoshihiko

doi:10.1016/j.placenta.2018.10.001

Cited by 2 publications

(2 citation statements)

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“…The iPSCs are considered as potential cell models for studying the regulation of cell differentiation, 19 disease pathogenesis, 8 , 20 , 21 , 22 , 23 , 24 organ regeneration, 25 , 26 stem cell therapy, 27 , 28 , 29 and drug screening. 30 , 31 , 32 , 33 , 34 Efficient and safe induction of CV cells is the key approach for the establishment and later application of iPSCs. The pEP4‐E02S‐ET2K plasmid, encompassing the human OCT4, S0X2, SV4 0LT, and KLF4 genes, used in the present study combined with the pCEP4‐miR‐302–367 plasmid, encompassing the miR‐302/367 sequences, do not carry c‐Myc oncogene, which exhibits carcinogenic effects.…”

Section: Discussionmentioning

confidence: 99%

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Establishment of a non‐integrated induced pluripotent stem cell line derived from human chorionic villi cells

Long

Shi

Sun

et al. 2022

Clinical Laboratory Analysis

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Background Few studies have investigated the generation of induced pluripotent stem cells (iPSCs) derived from human primary chorionic villi (CV) cells. The present study aimed to explore an optimal electroporation (EP) condition for generating non‐integrated iPSCs from CV cells (CV‐iPSCs). Methods The EGFP plasmid was transfected into CV cells under different EP conditions to evaluate cell adherence and the rate of EGFP positive cells. Subsequently, CV cells were transfected with the pEP4‐E02S‐ET2K and pCEP4‐miR‐302–367 plasmids under optimal EP conditions. Finally, CV‐iPSC pluripotency, karyotype analysis, and differentiation ability were investigated. Results Following EP for 48 h under different conditions, different confluency, and transfection efficiency were observed in CV cells. Higher cell density was observed in CV cells exposed to 200 V for 100 s, while higher transfection efficiency was obtained in cells electroporated at a pulse of 300 V for 300 s. To generate typical primitive iPSCs, CV cells were transfected with pEP4‐E02S‐ET2K and pCEP4‐miR‐302–367 plasmids using EP and were then cultured in induction medium for 20 days under selected conditions. Subsequently, monoclonal iPSCs were isolated and were evaluated pluripotency with AP positive staining, the expression of OCT4, SOX2, and NANOG in vitro and the formation of three germ layer teratomas in vivo. Conclusion CV‐iPSCs were successfully established under the conditions of 100 μl shock cup and EP pulse of 200 V for 300 s for two times. This may provide a novel strategy for investigating the pathogenesis of several diseases and gene therapy.

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Section: Discussionmentioning

confidence: 99%

“…The iPSCs are considered as potential cell models for studying the regulation of cell differentiation, 19 disease pathogenesis, 8,20–24 organ regeneration, 25,26 stem cell therapy, 27–29 and drug screening 30–34 . Efficient and safe induction of CV cells is the key approach for the establishment and later application of iPSCs.…”

Section: Discussionmentioning

confidence: 99%