Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species

Abstract

Many members of the genus Artemisia are important for medicinal purposes with multiple pharmacological properties. Often, these herbal plants sold on the markets are in processed forms so it is difficult to authenticate. Routine testing and identification of these herbal materials should be performed to ensure that the raw materials used in pharmaceutical products are suitable for their intended use. In this study, five commonly used Artemisia species included Artemisia argyi, Artemisia annua, Artemisia lavandulaefolia, Artemisia indica, and Artemisia atrovirens were analyzed using high resolution melting (HRM) analysis based on the internal transcribed spacer 2 (ITS2) sequences. The melting profiles of the ITS2 amplicons of the five closely related herbal species are clearly separated so that they can be differentiated by HRM method. The method was further applied to authenticate commercial products in powdered. HRM curves of all the commercial samples tested are similar to the botanical species as labeled. These congeneric medicinal products were also clearly separated using the neighbor-joining (NJ) tree. Therefore, HRM method could provide an efficient and reliable authentication system to distinguish these commonly used Artemisia herbal products on the markets and offer a technical reference for medicines quality control in the drug supply chain.

Study of Commercially Available Lobelia chinensis Products Using Bar-HRM Technology

“…HRM technology has been utilized for discriminating cultivated varieties, medicinal plant species, herbal tea, wine authenticity (Jung et al, 2010; Osathanunkul et al, 2015, 2016; Costa et al, 2016; Li et al, 2016; Song et al, 2016; Pereira et al, 2017). However, a reliable HRM approach to identify herbal medicines on Chinese herb market is still lacks.…”

Botanical origin authentication of dietary supplements by DNA‐based approaches

“…The method allowed the rapid detection and differentiation of seven Sideritis species, without the need of sequencing. Song, Li, Xiong, Liu and Liang (2016) developed a similar approach that clearly differentiated five Artemisia species ( A. argyi , A. annua , A. lavandulaefolia , A. indica , and A. atrovirens ) used for medicinal purposes. The method was further applied to authenticate commercial powdered products, showing that all the botanical species were according to the label.…”

Comparison of Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium by simultaneous determination of multi‐components with single reference standard method and chemometric analysis

“…Intriguingly, the two herbal medicines derived from the Artemisia genus have similar morphological effects, but the pharmacological effects and clinical applications are different. According to the theory of traditional Chinese medicine, Artemisiae argyi Folium was reported to possess immuno‐modulatory properties and commonly used for treatment of haemorrhage, pain, and skin itch, while Artemisiae lavandulaefoliae Folium has remarkable effects on menstruation‐related symptoms and was usually used in curing Gong cold infertility and irregular menstruation . The different efficacy of the two Artemisia herbal medicines has been believed to be correlated with the chemical profiling and the contents of bioactive compounds.…”

“…[19][20][21] To comprehensively compare and evaluate the composition of Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium, and find the different non-volatile compounds between the two Artemisia herbal medicines, in this work, a method based on a single reference standard for simultaneous determination of multi-components combined with chemometrics analysis was developed. The developed method was validated in terms of linearity, precision, repeatability, (6), jaceosidin (7) and eupatilin (8) were purchased from Chengdu Must Bio-technology Co., Ltd (Chengdu, China). The structures of the eight reference compounds are shown in Figure 1.…”

“…Taking into account feasibility and economy, the single reference standard should be a common constituent in all test samples and easily available. In this study, 3-caffeoylquinic acid (2) was chosen as the reference standard to calculate RRFs and RRT of the other seven compounds(1,(3)(4)(5)(6)(7)(8) owing to its cheapness, appropriate retention time and good separation. The RRFs and RRT were calculated as…”