Applying Advanced
            <scp>DNA</scp>
            Assembly Methods to Generate Pathway Libraries

Eriksen, Dawn T.; Chao, Ran; Zhao, Huimin

doi:10.1002/9783527688104.ch16

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2020

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“…Construction of synthetic genomes will first entail the utilization of commonly used in vitro and/or in vivo genetic assembly methods ( Eriksen et al, 2018 ; Figure 2A ). The initial optimization of gene cluster architecture can be performed by combinatorial variation of part choice, gene order, gene orientation, and operon occupancy ( Figure 2B ), as it was shown that these factors are highly useful for genetic optimization ( Smanski et al, 2014 ).…”

Section: Challenges and Possible Solutionsmentioning

confidence: 99%

Roadmap to Building a Cell: An Evolutionary Approach

Abil

Danelon

2020

Front. Bioeng. Biotechnol.

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Laboratory synthesis of an elementary biological cell from isolated components may aid in understanding of the fundamental principles of life and will provide a platform for a range of bioengineering and medical applications. In essence, building a cell consists in the integration of cellular modules into system's level functionalities satisfying a definition of life. To achieve this goal, we propose in this perspective to undertake a semirational, system's level evolutionary approach. The strategy would require iterative cycles of genetic integration of functional modules, diversification of hereditary information, compartmentalized gene expression, selection/screening, and possibly, assistance from open-ended evolution. We explore the underlying challenges to each of these steps and discuss possible solutions toward the bottom-up construction of an artificial living cell.

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Section: Challenges and Possible Solutionsmentioning

confidence: 99%