Applications of viral nanoparticles in medicine

Yildiz, İbrahim; Shukla, Sourabh; Steinmetz, Nicole F.

doi:10.1016/j.copbio.2011.04.020

Cited by 264 publications

(237 citation statements)

References 76 publications

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“…In recent years, protein and viral nanoparticles and nanostructured materials have begun to be developed for a myriad of biomedical uses (for some reviews see (1)(2)(3)(4)) and nanotechnological applications (for some reviews see (5)(6)(7)(8)). Inspired by natural protein complexes, self-assembly competence is also being engineered in other proteins and biomolecules (7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Kinetics of Surface-Driven Self-Assembly and Fatigue-Induced Disassembly of a Virus-Based Nanocoating

Valbuena

Mateu

2017

Biophysical Journal

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Self-assembling protein layers provide a ''bottom-up'' approach for precisely organizing functional elements at the nanoscale over a large solid surface area. The design of protein sheets with architecture and physical properties suitable for nanotechnological applications may be greatly facilitated by a thorough understanding of the principles that underlie their self-assembly and disassembly. In a previous study, the hexagonal lattice formed by the capsid protein (CA) of human immunodeficiency virus (HIV) was self-assembled as a monomolecular layer directly onto a solid substrate, and its mechanical properties and dynamics at equilibrium were analyzed by atomic force microscopy. Here, we use atomic force microscopy to analyze the kinetics of self-assembly of the planar CA lattice on a substrate and of its disassembly, either spontaneous or induced by materials fatigue. Both selfassembly and disassembly of the CA layer are cooperative reactions that proceed until a phase equilibrium is reached. Self-assembly requires a critical protein concentration and is initiated by formation of nucleation points on the substrate, followed by lattice growth and eventual merging of CA patches into a continuous monolayer. Disassembly of the CA layer showed hysteresis and appears to proceed only after large enough defects (nucleation points) are formed in the lattice, whose number is largely increased by inducing materials fatigue that depends on mechanical load and its frequency. Implications of the kinetic results obtained for a better understanding of self-assembly and disassembly of the HIV capsid and protein-based two-dimensional nanomaterials and the design of anti-HIV drugs targeting (dis)assembly and biocompatible nanocoatings are discussed.

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Section: Introductionmentioning

confidence: 99%

Kinetics of Surface-Driven Self-Assembly and Fatigue-Induced Disassembly of a Virus-Based Nanocoating

Valbuena

Mateu

2017

Biophysical Journal

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“…To date, there are many non-polymeric genetically engineered drug carriers that have been developed and applied extensively in gene and drug delivery. 7,14,[45][46][47][48][49] In this review of nanometer-scale drug carriers, we highlight two main categories of non-polymeric genetically engineered drug carriers that form useful nanostructures: vault proteins and viral proteins.…”

Section: Non-polymeric Drug Carriers and Their Structuresmentioning

confidence: 99%

“…Naturally, viruses can infect plants and animals effectively and transfer their genetic materials (DNA, RNA, or proteins) to the host cells. 47 Virus-like particles (VLPs) take advantage of this highly evolved and efficient transfer strategy to deliver their cargos by mimicking the natural process of viruses. VLPs have their own advantages: 1) milligram quantities of VLPs can be produced quickly and efficiently, which allows easy scale-up; 2) VLPs tend to be very robust because of their protein capsids and are stable in a range of solvents; and 3) VLPs possess great cell membrane penetration ability because of the viral features.…”

Section: Vault Proteinmentioning

confidence: 99%

Genetically engineered nanocarriers for drug delivery

Shi¹,

Gustafson²,

MacKay³

2014

IJN

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Cytotoxicity, low water solubility, rapid clearance from circulation, and off-target side-effects are common drawbacks of conventional small-molecule drugs. To overcome these shortcomings, many multifunctional nanocarriers have been proposed to enhance drug delivery. In concept, multifunctional nanoparticles might carry multiple agents, control release rate, biodegrade, and utilize target-mediated drug delivery; however, the design of these particles presents many challenges at the stage of pharmaceutical development. An emerging solution to improve control over these particles is to turn to genetic engineering. Genetically engineered nanocarriers are precisely controlled in size and structure and can provide specific control over sites for chemical attachment of drugs. Genetically engineered drug carriers that assemble nanostructures including nanoparticles and nanofibers can be polymeric or non-polymeric. This review summarizes the recent development of applications in drug and gene delivery utilizing nanostructures of polymeric genetically engineered drug carriers such as elastin-like polypeptides, silk-like polypeptides, and silk-elastin-like protein polymers, and non-polymeric genetically engineered drug carriers such as vault proteins and viral proteins.

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“…Recent advances in nanotechnology have facilitated the development of innovative products for diagnosis and therapy [5,6]. Nanomaterial-based vector systems exhibit low immunogenicity, easily tunable molecular weight and structure, and facile conjugation of functional moiety to the nanomaterial backbone.…”

mentioning

confidence: 99%

Evolving Lessons on Nanomaterial-Coated Viral Vectors for Local and Systemic Gene Therapy

Kasala

Yoon

Hong

et al. 2016

Nanomedicine (Lond.)

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Viral vectors are promising gene carriers for cancer therapy. However, virus-mediated gene therapies have demonstrated insufficient therapeutic efficacy in clinical trials due to rapid dissemination to nontarget tissues and to the immunogenicity of viral vectors, resulting in poor retention at the disease locus and induction of adverse inflammatory responses in patients. Further, the limited tropism of viral vectors prevents efficient gene delivery to target tissues. In this regard, modification of the viral surface with nanomaterials is a promising strategy to augment vector accumulation at the target tissue, circumvent the host immune response, and avoid nonspecific interactions with the reticuloendothelial system or serum complement. In the present review, we discuss various chemical modification strategies to enhance the therapeutic efficacy of viral vectors delivered either locally or systemically. We conclude by highlighting the salient features of various nanomaterial-coated viral vectors and their prospects and directions for future research.

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Applications of viral nanoparticles in medicine

Cited by 264 publications

References 76 publications

Kinetics of Surface-Driven Self-Assembly and Fatigue-Induced Disassembly of a Virus-Based Nanocoating

Kinetics of Surface-Driven Self-Assembly and Fatigue-Induced Disassembly of a Virus-Based Nanocoating

Genetically engineered nanocarriers for drug delivery

Evolving Lessons on Nanomaterial-Coated Viral Vectors for Local and Systemic Gene Therapy

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