Applications of Fluorescence Correlation Spectroscopy— Particle Size Effect

The performance of 2D array detectors for light sheet based fluorescence correlation spectroscopy

“…This correction is similar to the one used to correct for afterpulsing of APDs in confocal FCS [76]. A further factor of 1.2 − 1.3 stems from the non-vanishing size of the beads in relation to the focus size, as the used models assume point-like fluorophores [65]. Another problem is that Eq.…”

Coherent Anti-Stokes Raman Scattering Correlation Spectroscopy: Probing Dynamical Processes with Chemical Selectivity

“…Size Effect. The particle-size effect in FCS has been carefully investigated by Starchev et al 34 They pointed out that the characteristic diffusion time of particles with size comparable to the excitation volume is larger than that expected from the theory dealing with a point diffuser. It is interesting to examine the size effects in our CARS-CS measurements of the 0.175-µm beads of which the diameter is comparable to the lateral width (fwhm r ) 0.28 µm) of the excitation intensity profile.…”

Inverse-fluorescence correlation spectroscopy more information and less labeling

“…3, and the lower concentrations are taken as dilutions by a factor 2 from 0.6 nM. B) Normalized iFCS-curves from measurements on polystyrene microspheres of 100, 200 and 400 nm diameter, with corresponding diffusion times tau D of 7, 20 and 54 ms. As expected for particles that are too large to be considered point-like, the diffusion time increases slightly more than in direct proportion to the particle radius (10,16,17). using standard FCS, only the 26 percent increase of the diffusion time tau D would be observed.…”

“…Firstly, when the particles radius exceeds about 20 percent of the beam radius omega 0 , the diffusion time increases more than linearly with particles radius (10,16,17). This explains why the measured diffusion time of the particles in Figure 3b more than doubles when the particle radius is increased by a factor of two.…”