Application of Ultrasound on Monitoring the Evolution of the Collagen Fiber Reinforced nHAC/CS CompositesIn Vivo

Abstract

To date, fiber reinforce scaffolds have been largely applied to repair hard and soft tissues. Meanwhile, monitoring the scaffolds for long periods in vivo is recognized as a crucial issue before its wide use. As a consequence, there is a growing need for noninvasive and convenient methods to analyze the implantation remolding process in situ and in real time. In this paper, diagnostic medical ultrasound was used to monitor the in vivo bone formation and degradation process of the novel mineralized collagen fiber reinforced composite which is synthesized by chitosan (CS), nanohydroxyapatite (nHA), and collagen fiber (Col). To observe the impact of cells on bone remodeling process, the scaffolds were planted into the back of the SD rats with and without rat bone mesenchymal stem cells (rBMSCs). Systematic data of scaffolds in vivo was extracted from ultrasound images. Significant consistency between the data from the ultrasound and DXA could be observed (P < 0.05). This indicated that ultrasound may serve as a feasible alternative for noninvasive monitoring the evolution of scaffolds in situ during cell growth.

Effects of the bilayer nano-hydroxyapatite/mineralized collagen-guided bone regeneration membrane on site preservation in dogs

“…Chen et al. 46 also indicated that a chitosan (CS), nHA, and collagen fiber composite membrane might serve as a desired alternative for improving bone cell growth. Li et al.…”

“…A previous study by Jin et al 45 suggested that the efficacy of bone regeneration of collagen-HA bilayer membrane was superior to collagen-HA single-layer dense membrane on bone regeneration of SD rat cranial defects. Chen et al 46 also indicated that a chitosan (CS), nHA, and collagen fiber composite membrane might serve as a desired alternative for improving bone cell growth. Li et al 47 also suggested that HA in conjunction with a resorbable collagen membrane might be an acceptable alternative to repair the alveolar ridge deficiencies.…”

3D printing of human ear pinna using cartilage specific ink

“…Ultrasound sonography (USG) of the rats was performed on day 9. Ultrasound images were taken at D. Y. Patil hospital and Research Centre, Kolhapur by prosound α-10 premier diagnostic ultrasound system (1.1 mechanical indexes, 80 transmission gains) equipped with a 5-10 MHz probe [31,32] for ear pinna scans. Rats were anesthetized in a prone position with an exposed examination area.…”

Bedside, Benchtop, and Bioengineering: Physicochemical Imaging Techniques in Biomineralization

“…Mineralisation can be monitored in an animal model using ultrasound. [89] Researchers implanted mesenchymal stem cell-seeded 3D matrices into rats and were able to follow the osteogenesis, degradation, and calcification of these constructs over time. These techniques are still relatively uncommon in research practice but given that ultrasound does not rely upon ionising radiation and requires no sample preparation, it may become more ubiquitous in future.…”