Application of the Optode to Measurements of Surface Po2 And Pco2 of the Isolated Guinea-Pig Heart

Opitz, N.; Weigelt, H; Barankay, T.; Lübbers, D. W.

doi:10.1007/978-1-4684-8890-6_14

Oxygen Transport to Tissue — III

1978

DOI: 10.1007/978-1-4684-8890-6_14

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Application of the Optode to Measurements of Surface Po2 And Pco2 of the Isolated Guinea-Pig Heart

N. Opitz

H Weigelt

T. Barankay

et al.

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1983

1998

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Cited by 3 publications

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“…The oxygen concentration in various tissue has been measured polarographically with a microelectrode (Broelsh et al, 1978;Silver, 1979). Optical methods have also been developed by several investigators (Longmuir and Knoop, 1976;Millikan, 1937;Chance, 1976;Jobsis, 1977;Opitz et al, 1978). For instance, emission of fluorescence at 470 run from excitation light at 340 nm can be used to detect changes in the content of reduced pyridine nucleotides.…”

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confidence: 99%

The effect of intracellular oxygen concentration on lactate release, pyridine nucleotide reduction, and respiration rate in the rat cardiac tissue.

Araki¹,

Tamura²,

Yamazaki³

1983

Circulation Research

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SUMMARY. By measuring the absorbance change due to myoglobin oxygenation in hemoglobinfree isolated perfused rat hearts, we analyzed effects of perfusion pressure and heart rate upon the intracellular oxygen concentration. With Langendorff perfusion, the cardiac tissue was kept normoxic (above 50 ^M O 2 ) at aortic pressure above 50 cm H 2 O, but became hypoxic (8 /XM O 2 ) at 30 cm H 2 O. The increase in cardiac work, expressed as the product of peak systolic pressure and heart rate, increased oxygen consumption at aortic pressure of 50-200 cm H 2 O. The heart was kept normoxic under these conditions. Lactate release, oxygen consumption, and the oxidationreduction state of pyridine nucleotide were measured as a function of myoglobin oxygenation under various normoxic and anoxic conditions. Pyridine nucleotide fluorescence and lactate release started to increase as the intracellular oxygen concentration decreased to 6 and 10 ^M, respectively. Oxygen consumption was kept constant until the oxygen concentration decreased to 10 HM and slowed down below it. A close relationship between oxygen consumption and lactate release was observed. Infusions of epinephnne and norepinephrine under normoxic perfusion conditions increased cardiac work, oxygen consumption, and lactate release. More than 50% of myoglobin was then deoxygenated even under normoxic perfusion conditions. The increase in lactate release was ascribable to the increase in glycolytic flux caused by hypoxia. The change of pyridine nucleotide fluorescence by epinephrine was also explained by hypoxia in cardiac tissue. (Circ Res 53: 448-455, 1983)

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mentioning

confidence: 99%

The effect of intracellular oxygen concentration on lactate release, pyridine nucleotide reduction, and respiration rate in the rat cardiac tissue.

Araki¹,

Tamura²,

Yamazaki³

1983

Circulation Research

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“…For the purpose of elucidating the interaction between the 02 and CO2 diffusions into the red cell, the CO2 diffusion coefficient in hemoglobin (Hb) solution was measured as the first step. Recently, pH-sensitive fluorescence of 4-methylumbelliferone has been used by Lubbers and his associates (LUBBERS et a!., 1979;OPITz et al, 1978) for measuring the PC02 of an isolated guinea-pig heart. 4-Methylumbelliferone (4-MU) is one of the coumarin derivatives (7-hydroxy-4-methylcoumarin).…”

mentioning

confidence: 99%

“…Recently, pH-sensitive fluorescence of 4-methylumbelliferone has been used by Lubbers and his associates (LUBBERS et a!., 1979;OPITz et al, 1978) for measuring the PC02 of an isolated guinea-pig heart. 4-Methylumbelliferone (4-MU) is one of the coumarin derivatives (7-hydroxy-4-methylcoumarin).…”

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Diffusion coefficients of CO2 molecule and bicarbonate ion in hemoglobin solution measured by fluorescence technique.

1983

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: The diffusion process of CO2 within a thin layer of hemoglobin (Hb) solution was followed by pH-sensitive fluorescence of 4-methylumbelliferone. In the presence of sufficient carbonic anhydrase in the layer, the pH change was accelerated and determined only by the diffusion. The diffusion rate was reduced as the Hb concentration was increased. The pH rise observed in the CO2 diffusion out of the layer was slower than the pH fall caused by inward diffusion. However, the outward diffusion rate was faster than the inward diffusion rate in contrast to the pH change. The diffusion coefficients of molecular CO2 and bicarbonate ion were separately determined from the simulated and experimental P02-time curves by utilizing the non-linearity of the CO2 dissociation curve. The simulated curve was obtained from a numerical solution of the differential equation for diffusion by using the measured CO2 dissociation curve. The diffusion coefficient of CO2 was assumed to decrease exponentially with increasing Hb concentration. The diffusion coefficient of HCO3-was found to be reduced hyperbolically as the Hb concentration was increased. The diffusion coefficients of CO2 and HCO3-of 100% hemolysate were 0.34 x 10-5 and 0.14 x 10-5 cm2/sec, respectively. For the purpose of elucidating the interaction between the 02 and CO2 diffusions into the red cell, the CO2 diffusion coefficient in hemoglobin (Hb) solution was measured as the first step. Recently, pH-sensitive fluorescence of 4-methylumbelliferone has been used by Lubbers and his associates (LUBBERS et a!., 1979;OPITz et al., 1978) for measuring the PC02 of an isolated guinea-pig heart. 4-Methylumbelliferone (4-MU) is one of the coumarin derivatives (7-hydroxy-4-methylcoumarin). In the present paper, 4-MU fluorescence was applied to the measurement of CO2 diffusion rate in a thin Hb solution layer. The wavelength of the maximum intensity of 4-MU fluorescence is about 450 mm, which is considerably

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How to measure pO2 of tissue sealed in a transparent chamber:

Lübbers

1998

Sensors and Actuators B: Chemical

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Application of the Optode to Measurements of Surface Po2 And Pco2 of the Isolated Guinea-Pig Heart

Cited by 3 publications

References 4 publications

The effect of intracellular oxygen concentration on lactate release, pyridine nucleotide reduction, and respiration rate in the rat cardiac tissue.

The effect of intracellular oxygen concentration on lactate release, pyridine nucleotide reduction, and respiration rate in the rat cardiac tissue.

Diffusion coefficients of CO2 molecule and bicarbonate ion in hemoglobin solution measured by fluorescence technique.

How to measure pO2 of tissue sealed in a transparent chamber:

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