Application of PRP gel alone or in combination with guided bone regeneration does not enhance bone healing process: An experimental study in rabbits

Mesenchymal stem cell (MSC) transplantation has proved to be a promising strategy in cell therapy and regenerative medicine. Although their mechanism of action is not completely clear, it has been suggested that their therapeutic activity may be mediated by a paracrine effect. The main goal of this study was to evaluate by radiographic, morphometric and histological analysis the ability of mesenchymal stem cells derived from human adipose tissue (Ad-MSC) and their conditioned medium (CM), to repair surgical bone lesions using an in vivo model (rabbit mandibles). The results demonstrated that both, Ad-MSC and CM, induce bone regeneration in surgically created lesions in rabbit's jaws, suggesting that Ad-MSC improve the process of bone regeneration mainly by releasing paracrine factors. The evidence of the paracrine effect of MSC on bone regeneration has a major impact on regenerative medicine, and the use of their CM can address some issues and difficulties related to cell transplants. In particular, CM can be easily stored and transported, and is easier to handle by medical personnel during clinical procedures.

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“…Bilateral and bicortical surgical defects of 10 mm diameter were created in the mandibular angles [23]. Animals were divided in three groups.…”

Section: Methodsmentioning

confidence: 99%

Paracrine Effect of Mesenchymal Stem Cells Derived from Human Adipose Tissue in Bone Regeneration

2014

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“…Different studies have been described in animal models with BMPs, PRPs or mesenchymal stem cells (Boyne, 2001;Srouji et al, 2005;Pradel et al, 2006;Carter et al, 2008;Pieri et al, 2009;Miloro et al, 2010;Mooren et al, 2010;Kazakos et al, 2011). At the moment however, these options are of experimental character and have to be further investigated in clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Jaw cysts – Filling or no filling after enucleation? A review

Ettl

Gosau

Sader

et al. 2012

Journal of Cranio-Maxillofacial Surgery

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“…New bone formation was detected in a PRP grafted canine model in the first 2 months (Gerard et al, 2006;Gerard et al, 2007). In contrast, PRP gel does not enhance the bone healing process in mandibular defects in rabbits (Kazakos et al, 2011). The results of human studies are also inconsistent (Marx et al, 1998;Wiltfang et al, 2003;Raghoebar et al, 2005, Marukawa et al, 2010.…”

Section: Introductionmentioning

confidence: 99%

Effects of Choukroun’s platelet-rich fibrin on bone regeneration in combination with deproteinized bovine bone mineral in maxillary sinus augmentation: A histological and histomorphometric study

Zhang¹,

Tangl²,

Huber³

et al. 2012

Journal of Cranio-Maxillofacial Surgery

178

172

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Application of PRP gel alone or in combination with guided bone regeneration does not enhance bone healing process: An experimental study in rabbits

Cited by 35 publications

References 31 publications

Paracrine Effect of Mesenchymal Stem Cells Derived from Human Adipose Tissue in Bone Regeneration

Paracrine Effect of Mesenchymal Stem Cells Derived from Human Adipose Tissue in Bone Regeneration

Jaw cysts – Filling or no filling after enucleation? A review

Effects of Choukroun’s platelet-rich fibrin on bone regeneration in combination with deproteinized bovine bone mineral in maxillary sinus augmentation: A histological and histomorphometric study

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