Application of plasma jet to the inhibition of the proliferation of hepatic malignant cells via reactive oxygen species generation

Nguyen, Duc Ba; Mok, Young Sun; Huynh, Do Luoung; Jeong, Dong Kee; Lee, Wong Gyu

doi:10.1002/ppap.201800173

Cited by 7 publications

(5 citation statements)

References 44 publications

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“…The selective killing of cancer cells without damaging normal cells is the most important feature of these methods. Numerous studies showed that both CAP and PAM have a selective effect toward oral cancer, [16] hepatic cancer, [17,18] skin cancer, [19,20] glioblastoma, [21][22][23] breast cancer, [24,25] pancreatic cancer, [26,27] OC, [28,29] neuroblastoma, [30] prostate cancer, [31] head and neck cancer, [32] lung cancer, [33] osteosarcoma, [34] leukemia, [35,36] and colorectal cancer [37] cells.…”

mentioning

confidence: 99%

Plasma‐activated medium induces apoptosis in chemotherapy‐resistant ovarian cancer cells: High selectivity and synergy with carboplatin

Rasouli

Mehdian

Hajisharifi

et al. 2021

Plasma Processes & Polymers

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Recently, cold atmospheric pressure plasmas (CAP) have emerged as a promising oncotherapeutic modality, through physical and chemical effects. Here, for the first time, A2780 CP and SKOV-3 cells, relevant to ovarian cancer and GCs as normal ovarian cells were evaluated through CAP directly, indirectly, and concomitant modality of plasma activated medium (PAM) with common drugs to overcome chemotherapy resistance in ovarian cancer. Our results confirm the high potential and the stronger selectivity of PAM in comparison to CAP for the selected cell lines and selectivity mechanism was related to the pH and concentration of H2O2, NO2 -, and NO3reactive species in the plasma stimulated medium. Compared to the combination of common carboplatin (CAR) and paclitaxel (PTX) chemotherapy treatments, the PAM-based treatment is very promising for ovarian cancer treatment. Our data verify that PAM alone and in combination with carboplatin sensitizes cancer cells to carboplatin, inhibits the SOD1 gene, and selectively induces apoptosis accompanied with high expression of p53, Bax, and activation of Caspase-3.

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mentioning

confidence: 99%

Plasma‐activated medium induces apoptosis in chemotherapy‐resistant ovarian cancer cells: High selectivity and synergy with carboplatin

Rasouli

Mehdian

Hajisharifi

et al. 2021

Plasma Processes & Polymers

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“…The average electron density of the He plasma jet was estimated as the ratio of the equilibrium number of electrons in one voltage period to the corresponding consumed gas volume of He, as shown in equation (5), which is adapted from [47,48]. Generally, the current monitor measures current by electron flow in a wire; therefore, ions in the plasma bullets may be still accounted for electrical charge of the plasma bullets by this method.…”

Section: Effect Of Shielding Gases On Electron Density Of Plasma Jetmentioning

confidence: 99%

“…As a result, the jet temperature can be in the range of 30 °C-70 °C. Consequently, in case of required jet temperature is close the human body temperature (<40 °C) in order to avoid effects of temperature on cells during plasma treatment [50,51], large consumptions of noble gases are required in the case of without shielding gas [19,47].…”

Section: Comparison Of Noble Gas Consumption Between Plasma Jet Sourcesmentioning

confidence: 99%

Generation of cold atmospheric plasma jet by a coaxial double dielectric barrier reactor

Nguyen

Trinh

Mok

et al. 2020

Plasma Sources Sci. Technol.

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In this study, the generation of cold atmospheric plasma jet was investigated in a coaxial double dielectric barrier configuration conjugated with a microsecond voltage pulse generator. The advanced reactor comprised two coaxial dielectric tubes of different diameters with two ringshaped electrodes covering outside the larger tube. The electrodes were immersed in a liquid dielectric (i.e. transformer oil) in order to isolate them from the ambient air, which completely prevented the direct sparks formation between them within the investigated range of the applied voltage. The He discharge gas was fed to the inner tube while either air, N 2 , or O 2 as a shielding gas was introduced into the gap between the two dielectrics. The result revealed that with these shielding gases, the unwanted abnormal discharge in the inter-dielectric gap was extinguished and the plasma discharge occurred only in the He gas in the inner tube. Consequently, the electrical power delivered to the He discharges maintained at a level of around 1 W with a considerable variation in the applied voltage. Meanwhile, the discharge power of the unshielded jet was almost tripled as the applied voltage was increased from 7 to 10 kV. Interestingly, the cold plasma jet generated by the alternative reactor design in this study, which had the electron density up to 2.5 × 10 13 cm −3 , consumed relatively less He gas with less restriction on the high voltage range applied. The design also enhanced the possibility to control the plasma chemistry and jet temperature by changing the shielding gas. Therefore, the reactor is capable of producing a cold atmospheric plasma jet for the emerging plasma application such as plasma medicine and bio-applications.

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“…Li et al [25] found that PAM highly increased the efficacy of the chemotherapeutic agent cisplatin on hepatocellular carcinoma with cancer stem cell characteristics. Nyguen et al [27] demonstrated that PAM combined with gemcitabine triggered the deaths of hepatic malignant cells via ROS generation.…”

Section: Introductionmentioning

confidence: 99%

Plasma-Activated Media Produced by a Microwave-Excited Atmospheric Pressure Plasma Jet Is Effective against Cisplatin-Resistant Human Bladder Cancer Cells In Vitro

Jo,

Joh,

Bae

et al. 2024

IJMS

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Media exposed to atmospheric pressure plasma (APP) produce reactive oxygen and nitrogen species (RONS), with hydrogen peroxide (H2O2), nitrite (NO2−), and nitrate (NO3−) being among the most detected species due to their relatively long lifetime. In this study, a standardized microwave-excited (ME) APP jet (APPJ) source was employed to produce gaseous RONS to treat liquid samples. The source was a commercially available plasma jet, which generated argon plasma utilizing a coaxial transmission line resonator at the operating frequency of 2.45 GHz. An ultraviolet-visible spectrophotometer was used to measure the concentrations of H2O2 and NO3− in plasma-activated media (PAM). Three different types of media (deionized water, Hank’s balanced salt solution, and cell culture solution Dulbecco’s modified eagles medium [DMEM]) were utilized as liquid samples. Among these media, the plasma-treated DMEM was observed to have the highest levels of H2O2 and NO3−. Subsequently, the feasibility of using argon ME-APPJ-activated DMEM (PAM) as an adjuvant to enhance the therapeutic effects of cisplatin on human bladder cancer cells (T-24) was investigated. Various cancer cell lines, including T-24 cells, treated with PAM were observed in vitro for changes in cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. A viability reduction was detected in the various cancer cells after incubation in PAM. Furthermore, the study’s results revealed that PAM was effective against cisplatin-resistant T-24 cells in vitro. In addition, a possible connection between HER expression and cell viability was sketched.

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Application of plasma jet to the inhibition of the proliferation of hepatic malignant cells via reactive oxygen species generation

Cited by 7 publications

References 44 publications

Plasma‐activated medium induces apoptosis in chemotherapy‐resistant ovarian cancer cells: High selectivity and synergy with carboplatin

Plasma‐activated medium induces apoptosis in chemotherapy‐resistant ovarian cancer cells: High selectivity and synergy with carboplatin

Generation of cold atmospheric plasma jet by a coaxial double dielectric barrier reactor

Plasma-Activated Media Produced by a Microwave-Excited Atmospheric Pressure Plasma Jet Is Effective against Cisplatin-Resistant Human Bladder Cancer Cells In Vitro

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