Application of Physiologically Active Substances Isolated from Natural Resources to Pharmacological Studies.

Ohizumi, Yasushi

doi:10.1254/jjp.73.263

Cited by 56 publications

(35 citation statements)

References 129 publications

(35 reference statements)

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“…Among these, tyrosinase is a ratelimiting enzyme that triggers the conversion of Ltyrosine to dopaquinone, resulting in melanin production. [1][2][3] Large numbers of compounds from natural sources have been targeted for drug development in both pharmacological 4) and cosmetic research. 5) Natural extracts are being employed as new agents in functional cosmetics.…”

Section: Involvement Of Extracellular Signal-regulated Kinase In Nobimentioning

confidence: 99%

Involvement of Extracellular Signal-Regulated Kinase in Nobiletin-Induced Melanogenesis in Murine B16/F10 Melanoma Cells

Yoon

Lee

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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Section: Involvement Of Extracellular Signal-regulated Kinase In Nobimentioning

confidence: 99%

Involvement of Extracellular Signal-Regulated Kinase in Nobiletin-Induced Melanogenesis in Murine B16/F10 Melanoma Cells

Yoon

Lee

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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“…is a synthesized compound derived from a natural product, eudistomin D, which is isolated from Caribbean tunicate Eudistoma olivaceum (8,9). MBED is a powerful Ca 2+ releaser in skeletal muscle sarcoplasmic reticulum (SR) vesicles and interacts with the binding of caffeine to smooth muscle SR in a competitive manner (10,11).…”

Section: +mentioning

confidence: 99%

Imaging of Ca2+ Release by Caffeine and 9-Methyl-7-bromoeudistornin D and the Associated Activation of Large Conductance Ca2+-Dependent K+ Channels in Urinary Bladder Smooth Muscle Cells of the Guinea Pig

Ohi

Atsuki

Torii

et al. 2001

Japanese Journal of Pharmacology

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ABSTRACT-Ca2+ release by caffeine and 9-methyl-7-bromoeudistomin D (MBED) and the concomitant activation of large conductance Ca 2+-dependent K + (BK) channels were analyzed using confocal Ca 2+ imaging and whole cell voltage-clamp methods in guinea pig urinary bladder smooth muscle cells. Puff application of 3 or 10 mM caffeine for several seconds (2 -5 s) elicited a large increase in intracellular Ca]i) and induced a phasic outward current at a holding potential of -40 mV. The phasic outward current was the summation of spontaneous transient outward currents (STOCs) due to marked activation of BK channels and was followed by a short cessation of STOCs. ]i by MBED occurred mainly in superficial areas. Longer application of 100 mM MBED for 2 min did not induce significant global [Ca 2+ ]i increase but decreased the amount of Ca 2+ release and cell shortening during the subsequent application of 10 mM caffeine. These results indicate that short application of MBED releases Ca 2+ preferentially from superficial storage sites, presumably due to its slow approach to deeper sites. MBED may be a good pharmacological tool to manipulate selectively the superficial Ca 2+ stores related to STOCs.

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“…Large numbers of compounds from natural resources have provided not only useful pharmacological tools (Furukawa et al, 1993;Ohizumi, 1997;Obara et al, 2002) but also novel leading compounds for drug development (Liu, 1993). Flavonoids form a large family of natural products and are widely distributed in the plant kingdom.…”

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confidence: 99%

Nobiletin, a Citrus Flavonoid, Reverses Learning Impairment Associated withN-Methyl-D-aspartate Receptor Antagonism by Activation of Extracellular Signal-Regulated Kinase Signaling

Nakajima¹,

Yamakuni²,

Matsuzaki³

et al. 2007

J Pharmacol Exp Ther

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Recent studies have indicated that learning-induced activation of extracellular signal-regulated kinase (ERK) signaling via Nmethyl-D-aspartate (NMDA) receptors is required for consolidation of the resultant learning. These findings raise an idea that control of ERK signaling may be a potential target for treatment of cognitive dysfunction. Our recent studies have demonstrated that nobiletin, a polymethoxylated flavone from Citrus depressa, enhances cAMP/protein kinase A/ERK signaling in cultured rat hippocampal neurons and PC12D cells. Here, we, for the first time, present the evidence that this natural compound reverses learning impairment associated with NMDA receptor antagonism by activation of ERK in the hippocampus. Treatment with 50 mg/kg nobiletin reversed the NMDA receptor antagonist MK-801 (dizocilpine maleate)-induced learning impairment in mice. Western blot analysis also showed that nobiletin reversed MK-801-induced inhibition of learning-associated ERK activation in the hippocampus of the animals. Furthermore, consistent with these results, in cultured rat hippocampal neurons, nobiletin restored MK-801-induced impairment of NMDA-stimulated phosphorylation of ERK in a concentration-dependent manner. Taken together, the present study suggests that compounds that activate ERK signaling improve cognitive deficits associated with NMDA receptor hypofunction and that nobiletin may give us a new insight into therapeutic drug development for neurological disorders exhibiting cognitive impairment accompanied by a hypofunction of NMDA receptor-ERK signaling.

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Application of Physiologically Active Substances Isolated from Natural Resources to Pharmacological Studies.

Cited by 56 publications

References 129 publications

Involvement of Extracellular Signal-Regulated Kinase in Nobiletin-Induced Melanogenesis in Murine B16/F10 Melanoma Cells

Involvement of Extracellular Signal-Regulated Kinase in Nobiletin-Induced Melanogenesis in Murine B16/F10 Melanoma Cells

Imaging of Ca2+ Release by Caffeine and 9-Methyl-7-bromoeudistornin D and the Associated Activation of Large Conductance Ca2+-Dependent K+ Channels in Urinary Bladder Smooth Muscle Cells of the Guinea Pig

Nobiletin, a Citrus Flavonoid, Reverses Learning Impairment Associated withN-Methyl-D-aspartate Receptor Antagonism by Activation of Extracellular Signal-Regulated Kinase Signaling

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