Application of microwave assisted extraction in extracting Torbangun leaves (Coleus ambonicus, L.) and its effects on polyphenol and flavonoids content

Abstract

Flavonoids Microwave Assisted Extraction Polyphenols Torbangun Leaves Torbangun leaves (Coleus ambonicus, L.) contain polyphenol compounds, flavonoids and antioxidant compounds that can be obtained by extraction methods. However, with the conventional extraction method it has the disadvantage of long extraction time and requires a lot of solvents. Therefore, this study discusses the use of microwave assisted extraction (MAE) method to extract the leaves of Torbangun. This study uses two treatment factors on MAE i.e. power variations (100, 180 and 300 Watts) and extraction time (1, 2 and 3 minutes). This study aims to analyze the effect of MAE on the content of polyphenol compounds and flavonoids in the extraction process of Torbangun leaf. The results showed that the highest total phenol (4196.59 mg GAE/g extract) was found in the treatment of 300 watt of power with extraction time of 3-minutes with IC50 value of 9.89 mg/ml. The highest total flavonoid value was 300 watt of power with 1-minute extraction time which was 4.54 mg QE/g DW.

Antioxidant Properties of Different Types of Torbangun Herbal Tea

“…This suggests that ethanol is more effective in terms of extracting the antioxidant-contributing compounds, such as phenolics and flavonoids, in Torbangun compared to water. Hendrawan et al (2019) used distilled water (aquadest) as the extraction solvent to study the antioxidant activity of dried Torbangun leaves extract using microwave assisted extraction method. In that study, the lowest IC50 value is found at around 9000 ɥg/mL using the highest microwave power (300 watts) and the longest extraction time (3 minutes).…”